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Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

DOI :

10.3791/63395-v

11:11 min

February 11th, 2022

February 11th, 2022

3,865 Views

1Center for Innovative Medical Devices and Sensors (REDDI Lab), Clemson University, 2Department of Bioengineering, Clemson University, 3Swann Medicine, 4Student Health Services, Clemson University, 5Department of Chemical & Biomolecular Engineering, Clemson University, 6Department of Chemical & Biomolecular Engineering, University of Delaware

The protocol describes a SARS-CoV-2 diagnostic method that utilizes open-source automation to perform RT-qPCR molecular testing of saliva samples. This scalable approach can be applied to clinical public health surveillance as well as to increase the capacity of smaller university laboratories.

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SARS CoV 2

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