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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

DOI :

10.3791/4040-v

May 21st, 2012

May 21st, 2012

38,546 Views

1Department of Pediatrics, Emory University, 2Department of Pathology & Laboratory Medicine, Emory University

Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.

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Isolation

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