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Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function

DOI :

10.3791/63939-v

8:43 min

August 25th, 2022

August 25th, 2022

2,107 Views

1Department of Biomedical Engineering, Washington University in St. Louis, 2Department of Computer Science and Engineering, Washington University in St. Louis

The present protocol describes the generation of Drosophila melanogaster expressing eNpHR2.0 or ReaChR opsins in the heart for OCT imaging and optogenetic heart pacing. Detailed instructions for Drosophila OCT imaging and heart beating modulation, including the simulation of restorable heart arrest, bradycardia, and tachycardia in live animals at different developmental stages, are reported.

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Drosophila Melanogaster Models

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