Decellularization of Porcine Flaps and Evaluation of Decellularization
9:06
Results: Perfusion-Decellularization of Porcine Vascularized Flaps
10:16
Conclusion
Transcript
This method describes the surgical procurement of three vascularized porcine flaps and their subsequent perfusion decellularization. This method can aid efforts to regenerate porcine flaps using a tissue decellularization and recellularization app
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The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.