Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
2.1K Views
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08:50 min
•
June 9th, 2022
DOI :
June 9th, 2022
•1Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, 2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 3Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 4Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto
Chapters in this video
0:05
Introduction
0:38
Procurement of Rat Hindlimb
2:21
Decellularization of Rat Hindlimbs
5:49
Post-Decellularization Washing and Sterilization
6:55
Results: Histological Staining and DNA Quantification of Native and Decellularized Rat Hindlimb Tissues
8:13
Conclusion
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