November 11th, 2022
•Cranial windows have become a ubiquitously implemented surgical technique to allow for intravital imaging in transgenic mice. This protocol describes the use of a surgical robot that performs semi-automated bone drilling of cranial windows and can help reduce surgeon-to-surgeon variability and partially mitigate thermal blood-brain barrier damage.
Related Videos
Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities
Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
Piezo High Accuracy Surgical Osteal Removal (PHASOR): A Technique for Improved Cranial Window Surgery in Mice
Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice
Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice
Assessment of Sensory Thresholds in Dogs Using Mechanical and Hot Thermal Quantitative Sensory Testing
Dosage-Adjusted Resistance Training in Mice with a Reduced Risk of Muscle Damage
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved