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In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

DOI :

10.3791/64224-v

6:45 min

August 4th, 2022

August 4th, 2022

4,549 Views

1Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine, University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo

The present protocol describes making a large (6 x 3 mm2) cranial window using food wrap, transparent silicone, and cover glass. This cranial window allows in vivo wide-field and two-photon calcium imaging experiments in the same mouse.

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In Vivo

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