Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

5.3K Views

12:14 min

September 14th, 2022

DOI :

10.3791/64574-v

September 14th, 2022


Transcript

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Subcellular Fractionation

Chapters in this video

0:04

Introduction

1:37

Mouse Brain Excision

3:32

Synaptosome Preparation

5:23

Hypotonic Lysis

6:24

Synaptic Vesicle Isolation

7:35

Synaptic Plasma Membrane Isolation

10:02

Results: Electron Microscopy of Synaptosomes and Immunoblot Analysis of Subcellular Fractions

11:00

Conclusion

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