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Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

DOI :

10.3791/60113-v

March 11th, 2020

March 11th, 2020

5,959 Views

1Synaptic Dysfunction Group, Neuroscience Research Center, Charité - University Medicine

We present a protocol to evaluate the balance between glutamate release and clearance at single corticostriatal glutamatergic synapses in acute slices from adult mice. This protocol uses the fluorescent sensor iGluu for glutamate detection, a sCMOS camera for signal acquisition and a device for focal laser illumination.

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Keywords Single Synapse

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