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Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

DOI :

10.3791/57223-v

9:17 min

March 14th, 2018

March 14th, 2018

9,856 Views

1Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia

This protocol provides a workflow on how to combine artificial microRNA-mediated RNA interference with optogenetics to stimulate specifically presynaptic boutons with reduced expression of selective gene(s) within intact neuronal circuits.

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Optogenetics

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