Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay
3.5K Views
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05:44 min
•
November 25th, 2022
DOI :
November 25th, 2022
•1Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, 2Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, 3Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University
Chapters in this video
0:05
Introduction
0:36
Plant Culture
1:20
Tissue Preparation and Pretreatment
2:37
Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production
3:42
Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge
5:07
Conclusion
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