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Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

DOI :

10.3791/65156-v

February 24th, 2023

February 24th, 2023

2,843 Views

1Department of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zurich

Here, we describe an improvement of the semi-in vitro (SIV) method for observing pollen tube guidance and reception in Arabidopsis thaliana, which increases the receptivity of ovules. The high-throughput SIV cum septum method may be coupled with gametophyte marker lines and genetically encoded biosensors to monitor the dynamic process of fertilization.

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