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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

DOI :

10.3791/56587-v

8:13 min

October 17th, 2017

October 17th, 2017

27,787 Views

1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences, The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences, The Ohio State University

The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.

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Seed Sterilization

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