Effects of Desmodium caudatum on Gastrointestinal Hormones and Intestinal Flora in Rats with Gastritis

As an indispensable component of the treasure trove of traditional Chinese medicine, folk medical materials hold great significance for clinical practice and modernization of national medicine. This article aims to study the effects of D.caudatum in treating gastritis based on gastrointestinal hormones and intestinal flora, providing a basis for its rational clinical application. Numerous studies have reported that the occurrence and development of chronic gastritis are linked to the secretion of gastrointestinal hormones, such as gastrin.

It can be utilized as an indicator to evaluate the development level of chronic gastritis. Furthermore, lipid peroxidation products triggered by reactive oxides can activate inflammatory cells, leading to chronic gastritis. In 16S rRNA high-throughput gene sequencing, low resolution may make it challenging to distinguish strains and genera with highly similar sequences, leading to difficulties in classification at higher taxonomic levels, such as genus, family, and phylum.

For the ELISA kit method, sample pretreatment steps like dilution and washing are usually required, introducing potential errors and variabilities. To prepare a 5%sodium salicylate solution, weigh 2.5 grams of powder on an electronic scale. Dissolve the powder in a small amount of distilled water.

And dilute it to a total volume of 50 milliliters. For the preparation of a decoction of Desmodium caudatum weigh 128 grams of D.Caudatum. And place it into a round bottom flask containing 1 liter of distilled water.

Concentrate the mixture to a final volume of 100 milliliters. Based on treatment, divide the rats into different groups. After sacrificing the rat by cervical dislocation, in the serum sample, measure the contents of gastrin and malondialdehyde using ELISA on a microplate reader.

In the pathological study of the stomach wall compared to rats from the control group, model group exhibited mild gastric wall atrophy and mild inflammation. In contrast, rats treated with D.caudatum decoction presented no inflammation, metaplasia, or atrophy, indicating the decoction's therapeutic effect. Serum gastrointestinal hormone assays revealed that rats in model groups had higher malondialdehyde levels than control group rats, which significantly decreased in post-treatment.

Gastrin levels were lower in model group rats compared to the control group, but increased significantly in the decoction-treated group, suggesting the decoction's efficacy in modulating gastrointestinal hormones.