Collection and Digestion of Pulp Tissue for Cell Isolation
1:09
Cell Culture and Characterization of DPSCs
1:41
Representative Results
Transcript
Begynd med at sikre den udtrukne menneskelige tand på plads med tandpincet. Skær derefter tanden ved hjælp af en diamantskive, der er forbundet til et tandhåndstykke og en vandkølevæske. Brug en tandgraver til at fjerne pulpavævet fra tanden.
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This protocol emphasizes the extraction, culture, and preservation of multipotent stem cells from dental pulp through enzymatic digestion. Additionally, it demonstrates their potential to differentiate into osteoblasts, adipocytes, and chondrocytes, highlighting the importance of precision and consistency in the process.