Cryopreservation of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells at the Optimal Stage

493 Views

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07:03 min

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November 3rd, 2023

DOI :

10.3791/65888-v

November 3rd, 2023

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Xinyue Bai*¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Ting Zhang*¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xinyue Zhu¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xianyu Huang¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Haiyun Liu¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xiaoyan Ding⁸, Mei Jiang¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xiaodong Sun¹^,²^,³^,⁴^,⁵^,⁶^,⁷

¹Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, ²National Clinical Research Center for Eye Diseases, ³Shanghai Clinical Research Center for Eye Diseases, ⁴Shanghai Key Clinical Specialty, ⁵Shanghai Key Laboratory of Ocular Fundus Diseases, ⁶Shanghai Engineering Center for Visual Science and Photomedicine, ⁷Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, ⁸Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences

Transcript

Summary

Explore More Videos

Cryopreservation

Human Embryonic Stem Cells

Retinal Pigment Epithelial Cells

RPE Replacement Therapy

Cell Recovery Rate

Exponential Phase

Thawing Techniques

Stem cell based Therapies

Molecular Mechanism

Retinal Diseases

Differentiated Cells

5 ethynyl 2 deoxyuridine Labeling Assay

Chapters in this video

0:00

Introduction

1:07

Human Embryonic Stem Cell‐Derived Retinal Pigment Epithelial Cell Dissociation and Optimal Cryopreservation Stage Determination

4:11

Preservation of Human Embryonic Stem Cell‐Derived Retinal Pigment Epithelial Cells with Optimized Cryopreservation

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