Name: Author Spotlight: Insights into an Efficient Murine Maxillary Orthodontic Model Protocol
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This work was supported by the National Natural Science Foundation of China grant 82100982 to F.L.

The animal procedures in this study were reviewed and approved by the Ethical Committee of the West China School of Stomatology, Sichuan University (WCHSIRB-D-2017-041). Adult C57BL/6 mice were used in this study (see the Table of Materials). This protocol adds mechanical load to the right maxillary first molar (M1) for mesial movement where a highly coordinated bone remodeling process is induced by coupling of bone resorption and bone formation (Figure 1).
<p class="jov...

A Murine Maxillary Orthodontic Model Guide to Bone Remodeling Analysis

<ol>
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In this paper, we tried to describe the simplest orthodontic tooth movement protocol on murine maxillary model step by step to study the latent mechanisms of mechanical load-induced bone remodeling. Apart from research on bone remodeling, there are some other mainstream applications of this method: 1) methodological research on the acceleration of orthodontic tooth movement; 2) research on orthodontic root resorption; 3) biological mechanisms of orthodontic tooth movement and pain; 4) research on the transgenic model.</p...

Bone is a highly active reconstructed tissue that adapts its size, shape, and properties through the lifetime of the individual1,2. In addition to hormones, aging, nutrition, and other biological or biochemical factors3, the idea that mechanical load is the most determining factor has garnered general acceptance4,5. Under some circumstances with abnormal mechanical load, the imbalance between bone resorption and bone formation may lead to abnormal bone remodeling and bone disorders. Bone diseases such as disuse osteoporosis and bone loss during long-term bed rest or in the presence of microgravity at spaceflight have a close relationship with abnormal mechanical load6,7,8.
Mechanical load has also been used to treat bone-related diseases such as distraction treatment and orthodontic treatment. Distraction treatment has been used in developmental diseases such as craniosynostosis and mandibular hypoplasia9,10, while orthodontic treatment has been widely used to rectify abnormal teeth position and any malocclusion11. The core of orthodontic treatment is also the management of mechanical load. When the bone tissue is subjected to mechanical load, a highly coordinated bone remodeling process is induced by coupling of bone resorption followed by bone formation, which can move teeth to achieve the orthodontic purpose12,13.
Although orthodontic treatment has been widely applied for clinical practice, as our knowledge of the biological effects of mechanical load is limited, the results of orthodontic treatment are uncontrollable. To overcome these limitations, several animal models such as mouse, rat, rabbit, cat, dog, monkey, and pig have been established to investigate the underlying mechanism of mechanical load-induced bone remodeling (Table 1)14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32. Large animals such as dogs, monkeys, and pigs have some advantages over small animals in orthodontic operation-they have more human-like teeth and dentition so that the surgical procedure is easy to replicate in humans. Additionally, a wide view can reduce the operation difficulty and make it possible to apply a variety of orthodontic schemes33,34. However, large animals are difficult to obtain, leading to challenges related to sample size, and they are subject to ethical restrictions35. Furthermore, routine extraction procedures and complex instruments make the experiments difficult to perform due to which large animals are rarely used.
Under such circumstances, rodents are mainly used to establish orthodontic models. Among these models, rats and rabbits have lower operating difficulty and more tooth movement schemes compared with mice. However, the murine model has the unique advantage that there are a large number of genetically modified mice available, which is especially crucial for investigating the underlying mechanisms36. However, the murine model is the most difficult model to manipulate because of its small size. Reviewing the current methods, moving the first molar in the mesial direction is the only practical method for an orthodontic model. Two devices are mainly used to move the tooth-coil spring and elastic band. Using an elastic band is easier, but the orthodontic force varies greatly, which makes it difficult to obtain stable results.
Xu et al.15 have established a murine model with a coil spring on the mandible. However, due to the mobility of the mandible and the obstructive nature of the tongue, operation on the maxilla is always the first choice for both intraoperative and postoperative considerations. Taddei et al.16 described a more detailed protocol on the murine maxilla 10 years ago and more visual and pellucid details should be added. In summary, this protocol has systematically described a detailed orthodontic tooth movement protocol in a murine maxillary model to help researchers master the modeling method in a standardized way and enable the comparative evaluation between different studies.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Experimental Models: Mouse Lines</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>C57/B6J&#160;</td>
			<td>Gempharmatech Experimental Animals Company&#160;</td>
			<td>C57/B6J</td>
			<td></td>
		</tr>
		<tr>
			<td>Critical Commercial Assays</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Hematoxylin and Eosin Stain Kit</td>
			<td>Biosharp</td>
			<td>BL700B</td>
			<td></td>
		</tr>
		<tr>
			<td>Masson&#8217;s Trichrome Stain Kit</td>
			<td>Solarbio</td>
			<td>G1340</td>
			<td></td>
		</tr>
		<tr>
			<td>Instruments</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>27 G needle</td>
			<td>Chengdu Xinjin Shifeng Medical Apparatus &#38; Instruments Co. LTD.</td>
			<td>SB1-074(IV)</td>
			<td></td>
		</tr>
		<tr>
			<td>Adhesives</td>
			<td>Minnesota Mining and Manufacturing Co., Ltd.</td>
			<td>41282</td>
			<td></td>
		</tr>
		<tr>
			<td>Corkboard</td>
			<td>DELI Group Co., Ltd.</td>
			<td>8705</td>
			<td></td>
		</tr>
		<tr>
			<td>Cotton balls</td>
			<td>Haishi Hainuo Group Co.,&#160; Ltd.</td>
			<td>20120047</td>
			<td></td>
		</tr>
		<tr>
			<td>Cotton sticks</td>
			<td>Lakong Medical Devices Co., Ltd.</td>
			<td>M6500R</td>
			<td></td>
		</tr>
		<tr>
			<td>Customized coil spring</td>
			<td>Chengdu Mingxing Spring Co., Ltd.</td>
			<td>1109-02</td>
			<td></td>
		</tr>
		<tr>
			<td>Forceps</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>none</td>
			<td></td>
		</tr>
		<tr>
			<td>Light-cured fluid resin</td>
			<td>Shofu Dental Trading (SHANGHAI) Co., Ltd.</td>
			<td>518785</td>
			<td></td>
		</tr>
		<tr>
			<td>Light curer</td>
			<td>Liang Ya Dental Equipment Co., Ltd.</td>
			<td>LY-A180</td>
			<td></td>
		</tr>
		<tr>
			<td>Medical adhesive tapes&#160;</td>
			<td>Haishi Hainuo Group Co.,&#160; Ltd.</td>
			<td>0008-2014</td>
			<td></td>
		</tr>
		<tr>
			<td>Medical non-woven fabric</td>
			<td>Henan Yadu Industrial Co., Ltd.</td>
			<td>01011500018</td>
			<td></td>
		</tr>
		<tr>
			<td>Needle holders</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>none</td>
			<td></td>
		</tr>
		<tr>
			<td>Rubber bands</td>
			<td>Haishi Hainuo Group Co.,&#160; Ltd.</td>
			<td>32X1</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical scissors</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>none</td>
			<td></td>
		</tr>
		<tr>
			<td>Tweezers</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>none</td>
			<td></td>
		</tr>
	</tbody>
</table>

the establishment of a murine maxillary orthodontic model

Due to the lack of reproducible protocols for establishing a murine maxillary orthodontic model, we present a reliable and reproducible protocol to provide researchers with a feasible tool to analyze mechanical loading-associated bone remodeling. This study presents a detailed flowchart in addition to different types of schematic diagrams, operation photos, and videos. We performed this protocol on 11 adult wide-type C57/B6J mice and harvested samples on postoperative days 3, 8, and 14. The micro-CT and histopathological data have proven the success of tooth movements coupled with bone remodeling using this protocol. Furthermore, according to the micro-CT results on days 3, 8, and 14, we have divided bone modeling into three stages: preparation stage, bone resorption stage, and bone formation stage. These stages are expected to help researchers concerned with different stages to set sample collection time reasonably. This protocol can equip researchers with a tool to carry out regenerative analysis of bone remodeling.

The Establishment of a Murine Maxillary Orthodontic Model

We have performed the OTM surgery on 11 adult male mice (C57/BL6, 3 months old). They were euthanized for results on days 3, 8, and 14 post surgery. In these experiments, the right maxillary side is the operation side, while the left maxillary side is the control side. The micro-CT showed that there was a temporal consecutive increase in the distance between M1 and M2: 30 &#181;m, 70 &#181;m, and 110 &#181;m at days 3, 8, and 14 post surgery, respectively (Figure 4). The low-density periodon...

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Author Spotlight: Insights into an Efficient Murine Maxillary Orthodontic Model Protocol

Here we demonstrate step-by-step a manageable, orthodontic tooth movement protocol operated on a murine maxillary model. With the explicit explanation of each step and visual demonstration, researchers can master this model and apply it to their experimental needs with a few modifications.

Due to the lack of reproducible protocols for establishing a murine maxillary orthodontic model, we present a reliable and reproducible protocol to ...