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Electrophoretic Separation of Proteins

33.1K Views

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08:17 min

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June 12th, 2008

DOI :

10.3791/758-v

June 12th, 2008

•
1Proteomic Center, Keck Graduate Institute of Applied Life Sciences

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Electrophoretic Separation
Proteins
Protein Separation
Electrophoresis
Complex Mixtures
Subunit Compositions
Protein Samples
Purification Of Proteins
Polyacrylamide Gel Electrophoresis
Electrical Field
Pore Size
Acrylamide Concentration
Protein Charge
Protein Size
Protein Shape
Migration Rate
Laemmli Method
Discontinuous Gel Electrophoresis
Denaturing Conditions
Sodium Dodecyl Sulfate SDS

Chapters in this video

0:00

Title: Electrophoretic

0:45

Introduction

1:06

Pour the Separating Gel

2:44

Pour The Stacking Gel

3:55

Prepare The Sample and Load the Gel

6:24

Run the Gel

7:59

Conclusion

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