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In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging Video (Video) | JoVE

DOI :

10.3791/827-v

August 3rd, 2008

August 3rd, 2008

10,017 Views

1Division of Cardiovascular Medicine, Stanford University

In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.

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