ويتم تمويل هذا العمل من قبل إدارة التخدير في مستشفى الأطفال في ميشيغان.

1. تحضير المعدات <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ميد مضخة الانصهار للتسريب البروبوفول </li><li style=";text-align:right;direction:rtl"> مكان أنابيب التدفئة أنفي رغامي تحت الفراش </li><li style=";text-align:right;direction:rtl"> توصيل الكاميرا إلى نطاق الاختيار والتوجيه </li><li style=";text-align:right;direction:rtl"> ثلاث حركات تؤثر على عرض نطاق من خلال : <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> داخل وخارج. </li><li style=";text-align:right;direction:rtl"> التناوب باستخدام مناورة بكلتا اليدين. </li><li style=";text-align:right;direction:rtl"> انثناء وتمديد غيض من نطاقها. </li></ol></li></ol> 2. تجهيزات إضافية <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> الفنتانيل 0،5-1،0 ميكروغرام / كغ </li><li style=";text-align:right;direction:rtl"> 12F شفط القسطرة المطاطية الحمراء </li><li style=";text-align:right;direction:rtl"> غليكوبيرولات 0،1-0،2 ملغ </li><li style=";text-align:right;direction:rtl"> المعادن نفخ هوك </li><li style=";text-align:right;direction:rtl"> عفرين رذاذ الأنف </li><li style=";text-align:right;direction:rtl"> زيوت التشحيم </li><li style=";text-align:right;direction:rtl"> يدوكائين 2 ٪ او 4 ٪ للرش المنخر </li></ol> 3. تحضير المريض <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> قناع الحفاظ على أداء تحريض التهوية عفوية. </li><li style=";text-align:right;direction:rtl"> بدء الرابع وتعطي الفنتانيل والرابع غليكوبيرولات. </li><li style=";text-align:right;direction:rtl"> تعطي البروبوفول بلعة 1 كجم مغ / والبدء في ضخ البروبوفول 200 ميكروغرام / كغ / دقيقة. </li><li style=";text-align:right;direction:rtl"> عيون الشريط قبل الرش عفرين لتجنب اتساع التلاميذ. </li><li style=";text-align:right;direction:rtl"> رذاذ عفرين ويدوكائين في كلا فتحتي الأنف. </li><li style=";text-align:right;direction:rtl"> قناع تهوية المريض لتوزيع عفرين ويدوكائين بشكل أقصى. </li><li style=";text-align:right;direction:rtl"> تمرير قسطرة المطاط الأحمر في كلا فتحتي الأنف لفحص سالكية. </li><li style=";text-align:right;direction:rtl"> اختيار منخر أكثر براءة اختراع لنطاق الالياف البصرية. </li><li style=";text-align:right;direction:rtl"> الحفاظ على المطاطية الحمراء في الأنف مع الطرف الآخر في البلعوم السفلي لO 2. </li><li style=";text-align:right;direction:rtl"> استخدام الخطاف لنفخ المطاط الأحمر لربط الدوائر وإدارة 2 لتر / دقيقة من O 2 (لا ينفخ تدفقات عالية في البلعوم السفلي). </li></ol> 4. الالياف البصرية الفعلية التنبيب <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> اتخاذ قبالة موصل أنبوب أنفي رغامي. </li><li style=";text-align:right;direction:rtl"> أنبوب مقدما على طول الطريق حتى في نطاق أليافي مشحم. </li><li style=";text-align:right;direction:rtl"> أليافي غيض نظيفة مع الكحول </li><li style=";text-align:right;direction:rtl"> وضع منحنى طفيف في نهاية نطاق لمتابعة الكلمة من الأنف. </li><li style=";text-align:right;direction:rtl"> الخيط الالياف البصرية الى الحنجرة عمياء. </li><li style=";text-align:right;direction:rtl"> ابحث عن أي الهياكل التشريحية المعروفة. تجمد! </li><li style=";text-align:right;direction:rtl"> جعل الهياكل التشريحية في وسط الميدان والاحتفاظ بها هناك. </li><li style=";text-align:right;direction:rtl"> عندما تقترب من الحبال الصوتية ، وإعطاء بلعة الرابع البروبوفول 1 مغ / كغ. </li><li style=";text-align:right;direction:rtl"> مرة واحدة عن طريق الحبال ، وجعل منعطفا نحو الانخفاض في القصبة الهوائية. </li><li style=";text-align:right;direction:rtl"> متابعة حلقات القصبة الهوائية إلى مستوى جؤجؤ. </li><li style=";text-align:right;direction:rtl"> قد يتطلب إضافية بلعة البروبوفول الرابع 1 مغ / كغ. </li><li style=";text-align:right;direction:rtl"> تأكد من غيض نطاق الالياف البصرية في موقف محايد. </li><li style=";text-align:right;direction:rtl"> الخيط ETT. إذا كان يحصل على ما يصل علقت ETT في الحنجرة ، يعطي بدوره 360 درجة من الأنبوب لتدور عليه في القصبة الهوائية. </li></ol> 5. ممثل النتائج هذا الفيديو يظهر كفاءة تقنية الالياف البصرية للالتنبيب الاختيارية في طب الأطفال المرضى التي يتم بسهولة قابل للتعليم للسكان والزملاء واستنساخه. المفتاح الرئيسي هو الحفاظ على العفوية التهوية في جميع الأوقات. حسن إعداد المعدات قبل جهة غير مهمة أيضا.

<ol>
	<li>Kinouchi, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Management+of+difficult+pediatric+airway.">Management of difficult pediatric airway.</a> Masui. 55, 24-32 (2006).</li><li>Chen, Y. L., Wu, K. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Airway+management+of+patients+with+craniofacial+abnormalities:+10-year+experience+at+a+teaching+hospital+in+Taiwan.">Airway management of patients with craniofacial abnormalities: 10-year experience at a teaching hospital in Taiwan.</a> J Chin Med Assoc. 72, 468-470 (2009).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Practice+guidelines+for+management+of+the+difficult+airway:+an+updated+report+by+the+American+Society+of+Anesthesiologists+Task+Force+on+Management+of+the+Difficult+Airway.">Practice guidelines for management of the difficult airway: an updated report by the American Society of Anesthesiologists Task Force on Management of the Difficult Airway.</a> Anesthesiology. 98, 1269-1277 (2003).</li><li>Fiadjoe, J., Stricker, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pediatric+difficult+airway+management:+current+devices+and+techniques.">Pediatric difficult airway management: current devices and techniques.</a> Anesthesiol Clin. 27, 185-195 (2009).</li><li>Wheeler, M., Roth, A. G., Dsida, R. M., Rae, B., Seshadri, R., Sullivan, C. L., Heffner, C. L., Cote, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Teaching+residents+pediatric+fiberoptic+intubation+of+the+trachea:+traditional+fiberscope+with+an+eyepiece+versus+a+video-assisted+technique+using+a+fiberscope+with+an+integrated+camera.">Teaching residents pediatric fiberoptic intubation of the trachea: traditional fiberscope with an eyepiece versus a video-assisted technique using a fiberscope with an integrated camera.</a> Anesthesiology. 101, 842-846 (2004).</li><li>Roth, A. G., Wheeler, M., Stevenson, G. W., Hall, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+a+rigid+laryngoscope+with+the+ultrathin+fibreoptic+laryngoscope+for+tracheal+intubation+in+infants.">Comparison of a rigid laryngoscope with the ultrathin fibreoptic laryngoscope for tracheal intubation in infants.</a> Can J Anaesth. 41, 1069-1073 (1994).</li><li>Ovassapian, A., Dykes, M. H., Golmon, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+training+programme+for+fibreoptic+nasotracheal+intubation.+Use+of+model+and+live+patients.">A training programme for fibreoptic nasotracheal intubation. Use of model and live patients.</a> Anaesthesia. 38, 795-798 (1983).</li><li>Stevenson, G. W., Roth, A. G., Wheeler, M., Hall, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+the+Olympus+LF-P+fibreoptic+laryngoscope+by+trainees+in+paediatric+anaesthesia.">Use of the Olympus LF-P fibreoptic laryngoscope by trainees in paediatric anaesthesia.</a> Anaesthesia. 51, (1996).</li><li>Taguchi, S., Kusunoki, S., Fukuda, H., Hamada, H., Kawamoto, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Difficult+tracheal+intubation+using+airway+scope+in+a+pediatric+patient+with+Hunter+syndrome.">Difficult tracheal intubation using airway scope in a pediatric patient with Hunter syndrome.</a> Masui. 58, 1278-1281 (2009).</li></ol>

الإعلان عن أي تضارب في المصالح.

التنبيب يمكن الالياف البصرية في طب الأطفال المرضى صعوبة التنفس مع 1،2 يكون مهمة صعبة ، ووجود dysmorphisms القحفي تحديات إضافية لالتنبيب الرغامي 3. انه من الصعب تنفيذ هذا الإجراء في طب الأطفال المرضى من البالغين وذلك أساسا بسبب أصغر الأطفال المرضى في الممرات اله?...

<ul style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ميد مضخة الانصهار للتسريب البروبوفول </li><li style=";text-align:right;direction:rtl"> الفنتانيل 0،5-1،0 ميكروغرام / كغ </li><li style=";text-align:right;direction:rtl"> 12F شفط القسطرة المطاطية الحمراء </li><li style=";text-align:right;direction:rtl"> غليكوبيرولات 0،1-0،2 ملغ </li><li style=";text-align:right;direction:rtl"> المعادن نفخ هوك </li><li style=";text-align:right;direction:rtl"> عفرين رذاذ الأنف </li><li style=";text-align:right;direction:rtl"> زيوت التشحيم </li><li style=";text-align:right;direction:rtl"> يدوكائين 2 ٪ او 4 ٪ للرش المنخر </li></ul>

guidelines for elective pediatric fiberoptic intubation

مطلوب في كثير من الأحيان التنبيب الالياف البصرية في طب الأطفال المرضى لا سيما في الخطوط الجوية الصعبة للمرضى متلازمة المتلازمي أي بيار روبين. سوف الأطفال الصغيرة التشبع بسرعة كبيرة إذا تمت مقاطعة التهوية أساسا إلى ارتفاع معدل الأيض. نحن تصف المبادئ التوجيهية لإجراء التنبيب أليافي آمنة مع الحفاظ على التنفس التلقائي في جميع أنحاء الداخلي. تظهر الخطوات التي تتطلب استخدام مضخة البروبوفول ، الفنتانيل ، غليكوبيرولات ، القسطرة المطاطية الحمراء ، والمعادن هوك insuflation ، عفرين ، ومواد التشحيم ورذاذ يدوكائين.

Watch this Scientific Journal Video about Guidelines for Elective Pediatric Fiberoptic Intubation at JoVE.com

Guidelines for Elective Pediatric Fiberoptic Intubation

نحن تصف المبادئ التوجيهية لتنفيذ آمن وفعال التنبيب أليافي الانتخابية في طب الأطفال المرضى مع الحفاظ على التهوية عفوية.

مبادئ توجيهية لالتنبيب الاختيارية الالياف البصرية للأطفال

Fiberoptic intubation in pediatric patients is often required especially in difficult airways of syndromic patients i.e. Pierre Robin Syndrome. Small ...

guidelines-for-elective-pediatric-fiberoptic-intubation

Research

JoVE Journal

Medicine

17.3K Views.  St. Jude Children's Research Hospital. نحن تصف المبادئ التوجيهية لتنفيذ آمن وفعال التنبيب أليافي الانتخابية في طب الأطفال المرضى مع الحفاظ على التهوية عفوية.

Video: Guidelines for Elective Pediatric Fiberoptic Intubation

A Novel Rescue Technique for Difficult Intubation and Difficult Ventilation

We describe a novel non surgical technique to maintain oxygenation and ventilation in a case of difficult intubation and difficult ventilation, which works especially well with poor mask fit.
Can not intubate, can not ventilate" (CICV) is a potentially life threatening situation. In this video we present a simulation of the technique we used in a case of CICV where oxygenation and ventilation were maintained by inserting an endotracheal tube (ETT) nasally down to the level of the naso-pharynx while sealing the mouth and nares for successful positive pressure ventilation.
A 13 year old patient was taken to the operating room for incision and drainage of a neck abcess and direct laryngobronchoscopy. After preoxygenation, anesthesia was induced intravenously. Mask ventilation was found to be extremely difficult because of the swelling of the soft tissue. The face mask could not fit properly on the face due to significant facial swelling as well. A direct laryngoscopy was attempted with no visualization of the larynx. Oxygen saturation was difficult to maintain, with saturations falling to 80%. In order to oxygenate and ventilate the patient, an endotracheal tube was then inserted nasally after nasal spray with nasal decongestant and lubricant. The tube was pushed gently and blindly into the hypopharynx. The mouth and nose of the patient were sealed by hand and positive pressure ventilation was possible with 100% O2 with good oxygen saturation during that period of time. Once the patient was stable and well sedated, a rigid bronchoscope was introduced by the otolaryngologist showing extensive subglottic and epiglottic edema, and a mass effect from the abscess, contributing to the airway compromise. The airway was secured with an ETT tube by the otolaryngologist.This video will show a simulation of the technique on a patient undergoing general anesthesia for dental restorations.

We describe a technique to maintain oxygenation and ventilation using an endotracheal tube inserted nasally to the level of the naso-pharynx while sealing the mouth and nares for successful positive pressure ventilation.

تقنية الرواية لانقاذ التنبيب الصعبة والعسيرة التهوية

We describe a novel non surgical technique to maintain oxygenation and ventilation in a case of difficult intubation and difficult ventilation, which ...

A Simple Method of Mouse Lung Intubation

A simple procedure to intubate mice for pulmonary function measurements would have several advantages in longitudinal studies with limited numbers or expensive animal. One of the reasons that this is not done more routinely is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper we demonstrate a procedure that eliminates one of the major hurdles associated with this intubation, that of visualizing the trachea during the entire time of intubation. The approach uses a 0.5 mm fiberoptic light source that serves as an introducer to direct the intubation cannula into the mouse trachea. We show that it is possible to use this procedure to measure lung mechanics in individual mice over a time course of at least several weeks. The technique can be set up with relatively little expense and expertise, and it can be routinely accomplished with relatively little training. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice, thereby minimizing the number of mice needed and increasing the statistical power by using each mouse as its own control.

This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.

طريقة بسيطة لتركيب الانبوبة الرئة ماوس

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences between the murine and human respiratory systems, especially in their upper respiratory tracts (URT). In some models, these differences in the murine nasal cavity can have a significant impact on disease progression and presentation in the lower respiratory tract (LRT) when using intranasal instillation techniques, potentially limiting the usefulness of the mouse model to study these diseases. For these reasons, it would be advantageous to develop a technique to instill bacteria directly into the mouse lungs in order to study LRT disease in the absence of involvement of the URT. We have termed this lung specific delivery technique intubation-mediated intratracheal (IMIT) instillation. This noninvasive technique minimizes the potential for instillation into the bloodstream, which can occur during more invasive traditional surgical intratracheal infection approaches, and limits the possibility of incidental digestive tract delivery. IMIT is a two-step process in which mice are first intubated, with an intermediate step to ensure correct catheter placement into the trachea, followed by insertion of a blunt needle into the catheter to mediate direct delivery of bacteria into the lung. This approach facilitates a &#62;98% efficacy of delivery into the lungs with excellent distribution of reagent throughout the lung. Thus, IMIT represents a novel approach to study LRT disease and therapeutic delivery directly into the lung, improving upon the ability to use mice as surrogates to study human respiratory disease. Furthermore, the accuracy and reproducibility of this delivery system also makes it amenable to Good Laboratory Practice Standards (GLPS), as well as delivery of a wide range of reagents which require high efficiency delivery to the lung.

Intubation-mediated Intratracheal IMIT Instillation: A Noninvasive, Lung-specific Delivery System

Intubation-mediated intratracheal (IMIT) instillation of reagents is an excellent, noninvasive method for studying respiratory disease, as well as a method for instilling therapeutic reagents directly into the lung. It is a rapid and highly reproducible method which is suitable for preclinical testing.

بوساطة التنبيب داخل الرغامى (IMIT) تقطير: وموسع والرئة محدد نظام تسليم

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences ...

An Improved Method for Rapid Intubation of the Trachea in Mice

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity is a commonly used model to study both acute lung injury and fibrosis, and multiple methods have been developed for administering bleomycin (and other toxic agents) into the lungs. However, many of these approaches, such as transtracheal instillation, have inherent drawbacks, including the need for strong anesthetics and survival surgery. This paper reports a quick, reproducible method of intratracheal intubation that involves mild inhaled anesthesia, visualization of the trachea, and the use of a surrogate spirometer to confirm exposure. As a proof of concept, 8-12 week old C57BL/6 mice were administered either 2.0 U/kg of bleomycin or an equivalent volume of PBS, and both damage and fibrotic endpoints were measured post-exposure. This procedure allows researchers to treat a large cohort of mice in a relatively short period with little expense and minimal post-procedure care.

This article presents a rapid and simple method for administering bleomycin directly into the mouse trachea via intubation. Key advantages of this method are that it is highly reproducible, easy to master, and does not require specialized equipment or lengthy recovery times.

افضل طريقة لرابيد تنبيب القصبة الهوائية في الفئران

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...

Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus

Ultrasound is a safe bedside imaging tool that obviates the use of ionizing radiation diagnostic procedures. Due to its convenience, the lung ultrasound has received increasing attention from neonatal physicians. Nevertheless, clear reference standards and guideline limits are needed for accurate application of this diagnostic modality. This document aims to summarize expert opinions and to provide precise guidance to help facilitate the use of the lung ultrasound in the diagnosis of neonatal lung diseases.

Lung ultrasound is a noninvasive and valuable tool for bedside evaluation of neonatal lung diseases. However, a relative lack of reference standards, protocols and guidelines may limit its application. Here, we aim to develop a standardized neonatal lung ultrasound diagnostic protocol to be used in clinical decision-making.

البروتوكول والمبادئ التوجيهية للرئة نقطة من الرعاية بالموجات فوق الصوتية في تشخيص الأمراض الرئوية الولدان استناداً إلى توافق آراء الخبراء الدوليين

Ultrasound is a safe bedside imaging tool that obviates the use of ionizing radiation diagnostic procedures. Due to its convenience, the lung ultrasound ...

Repeated Orotracheal Intubation in Mice

The literature describes several methods for mouse intubation that either require visualization of the glottis through the oral cavity or incision in the ventral neck for direct confirmation of cannula placement in the trachea. The relative difficulty or the tissue trauma induced to the subject by such procedures can be an impediment to an investigator&#8217;s ability to perform longitudinal studies. This article illustrates a technique in which physical manipulation of the mouse following the use of a depilatory to remove hair from the ventral neck permits transcutaneous visualization of the trachea for orotracheal intubation regardless of degree of skin pigmentation. This method is innocuous to the subject and easily achieved with a limited understanding of murine anatomy. This refined approach facilitates repeated intubation, which may be necessary for monitoring progression of disease or instillation of treatments. Using this method may result in a reduction of the number of animals and technical skill required to measure lung function in mouse models of respiratory disease.

The goal of this article is to describe a refined method of intubation of the laboratory mouse. The method is noninvasive and, therefore, ideal for studies that require serial monitoring of respiratory function and/or instillation of treatments into the lung.

التنبيب المتكرر للأوتراكشفاء في الفئران

The literature describes several methods for mouse intubation that either require visualization of the glottis through the oral cavity or incision in the ...

Blind Endotracheal Intubation in Neonatal Rabbits

The newborn rabbit is a useful animal model for various pathologies and procedures. Airway management of the rabbit is complex due to its anatomical characteristics, which is further complicated in the case of the newborn. Of the different methods of advanced airway management, endotracheal intubation is less aggressive than tracheostomy, and is more feasible than supraglottic management given the lack of supraglottic devices of such a small size. As direct glottis visualization is very difficult in animals this size, this blind intubation model is presented as an effective alternative, especially for experiments requiring prolonged anesthesia. Using this method, we performed blind intubations with a 90% success rate.

We describe a technique of endotracheal intubation in newborn rabbits after esophageal catheterization with a gastric tube.

التنابيب Endotracheal الأعمى في الأرانب حديثي الولادة

The newborn rabbit is a useful animal model for various pathologies and procedures. Airway management of the rabbit is complex due to its anatomical ...

Endotracheal Intubation Using a Flexible Intubation Endoscope as a Standardized Model for Safe Airway Management in Swine

Endotracheal intubation is often a basic requirement for translational research in porcine models for various interventions that require a secured airway or high ventilation pressures. Endotracheal intubation is a challenging skill,&#160;requiring a minimum number of successful endotracheal intubations to achieve a high success rate under optimal conditions, which is often unachievable for non-anaesthesiology researchers. Due to the specific porcine airway anatomy, a difficult airway can usually be assumed. The impossibility of establishing a secure airway can result in injury, adverse events, or death of the laboratory animal. Using a prospective, randomized, controlled evaluation approach, it has been shown that fiberoptic-assisted endotracheal intubation takes longer but has a higher first-pass success rate than conventional intubation without causing clinically relevant drops in oxygen saturation. This model presents a standardized regimen for endoscopically guided endotracheal intubation, providing a secured airway, especially for researchers who are inexperienced in the technique of endotracheal intubation via direct laryngoscopy. This procedure is expected to minimize animal suffering and unnecessary animal losses.

The use of pigs in research has increased in recent years. Nevertheless, pigs are characterized by difficult airway anatomy. By demonstrating how to perform endoscopically guided endotracheal intubation, the present protocol aims to further increase laboratory animals' safety to avoid animal suffering and unnecessary death.

التنبيب الرغامي باستخدام منظار التنبيب المرن كنموذج موحد لإدارة مجرى الهواء الآمن في الخنازير

Endotracheal intubation is often a basic requirement for translational research in porcine models for various interventions that require a secured airway ...

Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

The dissection of murine neonatal bile ducts has been described as difficult. The main aim of the described standard operating procedure is the isolation of the extrahepatic bile duct (EBD) in mouse neonates without damaging the bile duct during preparation. Because of its exceptionally close preparation compared to the cholangiocytes cell line and harvesting of the entire extrahepatic bile duct system (EBDS), the described approach is extremely useful in researching animal models of newborn bile duct disorders, such as biliary atresia. After euthanasia, the peritoneal cavity was accessed, and the bile duct system, duodenum, and liver were extracted with the unique En-bloc-Resection (EbR). The extracted sample is placed on a foam mat, and the EBD is dissected from contaminating cells atraumatically without necessary touch. The dissection of the entire EBDS is a significant advantage of this method. Caution must be taken due to the small size and amount of bile duct tissue. Using the described technique, there is no damage to the cholangiocytes. Further, the purity of the technique is reproducible (n = 10). Therefore, optimally comparable samples can be harvested. Furthermore, no bile duct tissue is harmed, because any contact with the bile duct system can be avoided during preparation, leaving the bile fluid inside the gall bladder. Most importantly, while performing the final gall bladder and bile duct dissection, atraumatic microinstruments were used only slightly lateral of the bile duct without squeezing it. This is the key to a clean and intact sample, and essential for further histological investigation or the isolation of cholangiocytes. To summarize, the described innovative dissection technique enables especially inexperienced operators with the necessary equipment to isolate the EBDS as cleanly as possible.

For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.

القناة الصفراوية خارج الكبد وتسلخ المرارة في حديثي الولادة في الفأر البالغ من العمر تسعة أيام

The dissection of murine neonatal bile ducts has been described as difficult. The main aim of the described standard operating procedure is the isolation ...

الإنعاش القلبي الرئوي خارج الجسم في النموذج الحيواني بعد السكتة القلبية

Pediatric Animal Model of Extracorporeal Cardiopulmonary Resuscitation After Prolonged Circulatory Arrest

Congenital heart disease (CHD) is the most prevalent congenital malformation, with about one million births impacted worldwide per year. Comprehensive investigation of this disease requires appropriate and validated animal models. Piglets are commonly used for translational research due to their analogous anatomy and physiology. This work aimed to describe and validate a neonatal piglet model of cardiopulmonary bypass (CPB) with circulatory and cardiac arrest (CA) as a tool for studying severe brain damage and other complications of cardiac surgery. In addition to including a list of materials, this work provides a roadmap for other investigators to plan and execute this protocol. After experienced practitioners performed several trials, the representative results of the model demonstrated a 92% success rate, with failures attributed to small piglet size and variant vessel anatomy. Furthermore, the model allowed practitioners to select from a wide variety of experimental conditions, including varying times in CA, temperature alterations, and pharmacologic interventions. In summary, this method uses materials readily available in most hospital settings, is reliable and reproducible, and can be widely employed to enhance translational research in children undergoing heart surgery.

تسليط الضوء على المؤلف: محاكاة جراحة القلب للأطفال باستخدام نموذج خنزير صغير لحديثي الولادة

This protocol describes a neonatal porcine model of cardiopulmonary bypass (CPB), with circulatory and cardiac arrest as a tool for studying severe brain damage and other complications secondary to CPB.

نموذج حيواني للأطفال للإنعاش القلبي الرئوي خارج الجسم بعد توقف الدورة الدموية لفترات طويلة

Congenital heart disease (CHD) is the most prevalent congenital malformation, with about one million births impacted worldwide per year. Comprehensive ...