جيل من صنع الإنسان من الخلايا الجذعية المحفزة الدموية المحيطية باستخدام Lentiviral STEMCCA المتجهات

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26.4K Views

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12:03 min

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October 31st, 2012

DOI :

10.3791/4327-v

October 31st, 2012

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Andreia Gianotti Sommer¹, Sarah S. Rozelle¹, Spencer Sullivan², Jason A. Mills³, Seon-Mi Park¹, Brenden W. Smith¹, Amulya M. Iyer¹, Deborah L. French³, Darrell N. Kotton¹, Paul Gadue³, George J. Murphy¹, Gustavo Mostoslavsky¹

¹Center for Regenerative Medicine (CReM), Boston University School of Medicine, ²Department of Hematology, Children's Hospital of Philadelphia, ³Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia

Summary

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68 iPSCs PBMCs lentiviral excisable STEMCCA

Chapters in this video

0:05

Title

1:50

Isolation and Expansion of PBMCs

4:34

Transduction of PBMCs with STEMCCA Lentivirus

6:11

Plating Transduced Cells onto MEFs

8:41

Picking and Expansion of iPSC Clones

9:26

Results: Human Induced Pluripotent Stem Cells

11:26

Conclusion

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