نود أن نشكر مؤسسة فودافون الأمريكتين، ومؤسسة بيل وميليندا غيتس، ومؤسسة أبحاث ساو باولو (FAPESP) لتمويل هذا البحث. ونود أيضا أن نشكر العديد من أعضاء هيئة التدريس من قسم علم الحشرات في جامعة كاليفورنيا، ريفرسايد، لنصائحهم في هذا المشروع.

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H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automated+identification+of+optically+sensed+aphid+(Homoptera:+Aphidae)+wingbeat+waveforms.">Automated identification of optically sensed aphid (Homoptera: Aphidae) wingbeat waveforms.</a> Annals of the Entomological Society of America. 95 (1), 1-8 (2002).</li><li>Li, Z., Zhou, Z., Shen, Z., Yao, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automated+identification+of+mosquito+(diptera:+Culicidae)+wingbeat+waveform+by+artificial+neural+network.">Automated identification of mosquito (diptera: Culicidae) wingbeat waveform by artificial neural network.</a> Artificial Intelligence Applications and Innovations. , 483-489 (2005).</li><li>Moore, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Artificial+neural+network+trained+to+identify+mosquitoes+in+flight.">Artificial neural network trained to identify mosquitoes in flight.</a> Journal of insect behavior. 4 (3), 391-396 (1991).</li><li>Halevy, A., Norvig, P., Pereira, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+unreasonable+effectiveness+of+data.">The unreasonable effectiveness of data.</a> Intelligent Systems, IEEE. 24 (2), 8-12 (2009).</li><li>Banko, M., Brill, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitigating+the+paucity-of-data+problem+exploring+the+effect+of+training+corpus+size+on+classifier+performance+for+natural+language+processing.">Mitigating the paucity-of-data problem exploring the effect of training corpus size on classifier performance for natural language processing.</a> , 1-5 (2001).</li><li>Shotton, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+human+pose+recognition+in+parts+from+single+depth+images.">Real-time human pose recognition in parts from single depth images.</a> Communications of the ACM. 56 (1), 116-124 (2013).</li><li>Ilyas, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Classifying+insects+on+the+fly.">Classifying insects on the fly.</a> Ecological Informatics. , (2013).</li><li>Mack, Y. P., Rosenblatt, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multivariate+k-nearest+neighbor+density+estimates.">Multivariate k-nearest neighbor density estimates.</a> Journal of Multivariate Analysis. 9 (1), 1-15 (1979).</li><li>Benedict, M., Robinson, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+first+releases+of+transgenic+mosquitoes+an+argument+for+the+sterile+insect+technique.">The first releases of transgenic mosquitoes an argument for the sterile insect technique.</a> TRENDS in Parasitology. 19 (8), 349-355 (2003).</li><li>. Supporting Materials Available from: <a target="_blank" href="https://sites.google.com/site/insectclassification">https://sites.google.com/site/insectclassification</a></li><li>Chen, Y., Why, A., Batista, G., Mafra-Neto, A., Keogh, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flying+Insect+Classification+with+Inexpensive+Sensors.">Flying Insect Classification with Inexpensive Sensors.</a> Journal of Insect. 27 (5), 657-677 (2014).</li></ol>

تعلن الكتاب أنه ليس لديهم المصالح المالية المتنافسة.

الإطار الاستشعار / تصنيف الموصوفة هنا يسمح للتصنيف غير مكلفة وقابلة للمن الحشرات الطائرة. على دقة قابلة للتحقيق من قبل النظام هي جيدة بما فيه الكفاية للسماح تطوير المنتجات التجارية ويمكن أن تكون أداة مفيدة في البحث الحشري. إن الق...

فكرة تصنيف الحشرات تلقائيا باستخدام الصوت عرضية من رحلتهم يعود تاريخها إلى الأيام الأولى من أجهزة الكمبيوتر المتاحة تجاريا ومعدات تسجيل الصوت 1. ومع ذلك، فقد تم إحراز تقدم ضئيل على هذه المشكلة في العقود الفاصلة. ويمكن أن يعزى عدم إحراز تقدم في هذا السعي إلى عدة عوامل ذات الصلة. أولا، جعلت قلة من أجهزة الاستشعار فعالة لجمع البيانات صعبا. وقد استخدمت معظم الجهود لجمع البيانات الميكروفونات الصوتية 2-5. هذه الأجهزة هي حساسة للغاية لتصفية الضجيج والضوضاء المحيطة في البيئة، مما أدى إلى بيانات ضئيلة جدا وذات جودة منخفضة. الثانية، مما يضاعف هذه القضايا جودة البيانات هو حقيقة أن العديد من الباحثين قد حاولوا معرفة تصنيف نماذج معقدة للغاية، وخاصة الشبكات العصبية 6-8. محاولة لمعرفة نماذج تصنيف معقدة، مع مجرد عشرات الأمثلة،وصفة لالإفراط في تركيب. ثالثا، صعوبة الحصول على البيانات يعني أن حاول العديد من الباحثين على بناء نماذج تصنيف مع بيانات محدودة جدا، عدد قليل من الحالات 9 300 أو أقل. ومع ذلك، فمن المعروف أن لبناء نماذج تصنيف دقيقة، والمزيد من بيانات أفضل 10-13. ويتناول هذا العمل جميع القضايا الثلاث. البصرية (بدلا من الصوتية) يمكن استخدام أجهزة الاستشعار لتسجيل &quot;سليمة&quot; للطيران الحشرات من متر، مع الثابتية الكامل لتصفية الضوضاء والأصوات المحيطة. وقد سمحت هذه المجسات لتسجيل الملايين من حالات التدريب المسمى، أكثر بكثير من البيانات كل الجهود السابقة مجتمعة، وبالتالي يساعد على تجنب الجهود البحثية السابقة الإفراط المناسب التي يعاني منها. وهناك طريقة المبدئي هو مبين أدناه أن يسمح بإدراج معلومات إضافية في نموذج التصنيف. يمكن أن تكون هذه المعلومات الإضافية كما اليومية المعتادة كما وسهلة والحصول على الوقت من اليوم، ومع ذلك لا تزال تنتج مكاسب كبيرة في دقة النموذج. وأخيرا، أظهرت أن كميات هائلة من البيانات التي تم جمعها تسمح لنا للاستفادة من &quot;فاعلية غير معقولة من البيانات&quot; 10 لإنتاج المصنفات بسيطة ودقيقة وقوية. باختصار، انتقلت تحلق تصنيف الحشرات وراء مطالبات مشكوك فيها تم إنشاؤها في مختبر البحوث وجاهز الآن للنشر في العالم الحقيقي. وأجهزة الاستشعار والبرمجيات المقدمة في هذا العمل توفر أدوات قوية الباحثين في جميع أنحاء العالم لتسريع أبحاثهم.

<table border="0" cellpadding="0" cellspacing="0" width="1293">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Name of Material/ Equipment</td>
			<td style="width:237px;">Company</td>
			<td style="width:257px;">Catalog Number</td>
			<td style="width:471px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Audio Recorder: ICD-PX312</td>
			<td style="width:237px;">Sony</td>
			<td style="width:257px;">4-267-065-11(2)</td>
			<td>With a 8 GB microSD extra memory</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Insectary</td>
			<td style="width:237px;">Lee&#39;s Aquarium &#38; Pet Products.</td>
			<td style="width:257px;">20088 HerpHaven&#174;, Large Rectangle</td>
			<td style="width:471px;">14 1/2&#34; Long x 8 3/4&#34; Wide x 9 3/4&#34; high. Modified to house insects.&#160;</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:328px;">Laser Line Generator, 650nm (red)</td>
			<td style="width:237px;">Apinex (www.apinex.com)</td>
			<td style="width:257px;">LN60-650</td>
			<td style="width:471px;">5mW. This is a low powered laser, similar to a teachers lasers pointer</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:328px;">Photodiode Array</td>
			<td style="width:237px;">VISHAY SEMICONDUCTOR&#160; TEFD4300&#160; PIN PHOTODIODE, 650NM, 20DEG, T1</td>
			<td style="width:257px;">TEFD4300</td>
			<td style="width:471px;">We made a custom array of 15 of these Photodiodes wired in parallel</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;">Analogue to Digital Convertor Integrated Circuit&#160;</td>
			<td style="width:237px;">Custom made in our lab</td>
			<td style="width:257px;"></td>
			<td style="width:471px;">We made this item ourselves, but an easily available commercial product, Gino PCF8591 AD/DA Converter, provides the same functionality.&#160;</td>
		</tr>
	</tbody>
</table>

flying insect detection and classification with inexpensive sensors

ومن شأن نظام موسع وغير مكلفة يمكن أن تصنف بدقة الحشرات الطائرة لها انعكاسات هامة على الأبحاث الحشري، والسماح لتطوير العديد من التطبيقات المفيدة في النواقل ومكافحة الآفات لكل من علم الحشرات الطبي والزراعي. ونظرا لهذا، شهدت السنوات الستين الماضية العديد من الجهود البحثية المكرسة لهذه المهمة. حتى الآن، ومع ذلك، كان لا شيء من هذا البحث تأثير دائم. في هذا العمل، وتبين لنا أن أجهزة الاستشعار البصرية الزائفة الصوتية يمكن أن تنتج بيانات متفوقة. أن ميزات إضافية، سواء جوهري وخارجي لسلوك رحلة الحشرة، يمكن استغلالها لتحسين تصنيف الحشرات. أن النهج تصنيف النظرية الافتراضية يتيح للتعلم بكفاءة تصنيف النماذج التي هي قوية جدا إلى أكثر من تركيب، وإطار التصنيف العام يسمح لدمج بسهولة التعسفي عدد من الميزات. نحن لشرح النتائج مع التجارب واسعة النطاق التي تقزم جميع الأعمال السابقة كومبيNED، كما يقاس بعدد من الحشرات وعدد الأنواع التي تعتبر.

يتم عرض تجربتين هنا. لكل من التجارب، والبيانات المستخدمة وتم أخذ عينات بشكل عشوائي من مجموعة البيانات التي تحتوي على أكثر من 100،000 الكائنات. وتظهر التجربة الأولى قدرة المصنف المقترحة لتصنيف الأنواع المختلفة بدقة / الجنسين من الحش...

Watch this Scientific Journal Video about Flying Insect Detection and Classification with Inexpensive Sensors at JoVE.com

Flying Insect Detection and Classification with Inexpensive Sensors

اقترحنا نظام يستخدم غير مكلفة، موسع أجهزة الاستشعار البصرية الزائفة الصوتية تلقائيا وبدقة لكشف والعد، وتصنيف الحشرات الطائرة على أساس الصوت سفرهم.

تحلق كشف وتصنيف الحشرات مع مجسات رخيصة

An inexpensive, noninvasive system that could accurately classify flying insects would have important implications for entomological research, and allow ...

flying-insect-detection-and-classification-with-inexpensive-sensors

Research

JoVE Journal

Bioengineering

25.0K Views.  University of California, Riverside.  اقترحنا نظام يستخدم غير مكلفة، موسع أجهزة الاستشعار البصرية الزائفة الصوتية تلقائيا وبدقة لكشف والعد، وتصنيف الحشرات الطائرة على أساس الصوت سفرهم. 

Video: Flying Insect Detection and Classification with Inexpensive Sensors

Bacterial Detection &amp; Identification Using Electrochemical Sensors

Electrochemical sensors are widely used for rapid and accurate measurement of blood glucose and can be adapted for detection of a wide variety of analytes. Electrochemical sensors operate by transducing a biological recognition event into a useful electrical signal. Signal transduction occurs by coupling the activity of a redox enzyme to an amperometric electrode. Sensor specificity is either an inherent characteristic of the enzyme, glucose oxidase in the case of a glucose sensor, or a product of linkage between the enzyme and an antibody or probe.
Here, we describe an electrochemical sensor assay method to directly detect and identify bacteria. In every case, the probes described here are DNA oligonucleotides. This method is based on sandwich hybridization of capture and detector probes with target ribosomal RNA (rRNA). The capture probe is anchored to the sensor surface, while the detector probe is linked to horseradish peroxidase (HRP). When a substrate such as 3,3',5,5'-tetramethylbenzidine (TMB) is added to an electrode with capture-target-detector complexes bound to its surface, the substrate is oxidized by HRP and reduced by the working electrode. This redox cycle results in shuttling of electrons by the substrate from the electrode to HRP, producing current flow in the electrode.

We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.

كشف البكتيرية والاستدلال باستخدام مجسات الكهروكيميائية

Electrochemical  sensors are widely used for rapid and accurate measurement of blood glucose and  can be adapted for detection of a wide variety of ...

Glutamine Flux Imaging Using Genetically Encoded Sensors

Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. A tremendous variety of such sensors for biological molecules became available in the past 15 years, some of which became indispensable tools that are used routinely in many laboratories.
One of the exciting applications of genetically encoded sensors is the use of these sensors in investigating cellular transport processes. Properties of transporters such as kinetics and substrate specificities can be investigated at a cellular level, providing possibilities for cell-type specific analyses of transport activities. In this article, we will demonstrate how transporter dynamics can be observed using genetically encoded glutamine sensor as an example. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed.

This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.

الجلوتامين الجريان التصوير باستخدام مجسات مشفرة وراثيا

Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. A tremendous variety of such sensors for ...

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Miniaturization of analytical benchtop procedures into the micro-scale provides significant advantages in regards to reaction time, cost, and integration of pre-processing steps. Utilizing these devices towards the analysis of DNA hybridization events is important because it offers a technology for real time assessment of biomarkers at the point-of-care for various diseases. However, when the device footprint decreases the dominance of various physical phenomena increases. These phenomena influence the fabrication precision and operation reliability of the device. Therefore, there is a great need to accurately fabricate and operate these devices in a reproducible manner in order to improve the overall performance. Here, we describe the protocols and the methods used for the fabrication and the operation of a microfluidic-based electrochemical biochip for accurate analysis of DNA hybridization events. The biochip is composed of two parts: a microfluidic chip with three parallel micro-channels made of polydimethylsiloxane (PDMS), and a 3 x 3 arrayed electrochemical micro-chip. The DNA hybridization events are detected using electrochemical impedance spectroscopy (EIS) analysis. The EIS analysis enables monitoring variations of the properties of the electrochemical system that are dominant at these length scales. With the ability to monitor changes of both charge transfer and diffusional resistance with the biosensor, we demonstrate the selectivity to complementary ssDNA targets, a calculated detection limit of 3.8 nM, and a 13% cross-reactivity with other non-complementary ssDNA following 20 min&#160;of incubation. This methodology can improve the performance of miniaturized devices by elucidating on the behavior of diffusion at the micro-scale regime and by enabling the study of DNA hybridization events.

We present a microfluidic-based electrochemical biochip for DNA hybridization detection. Following ssDNA probe functionalization, the specificity, sensitivity, and detection limit are studied with complementary and non-complementary ssDNA targets. Results illustrate the influence of the DNA hybridization events on the electrochemical system, with a detection limit of 3.8 nM.

وبيوشيب الكهروكيميائية استنادا ميكروفلويديك لتسمية خالية تحليل الحمض النووي التهجين

Miniaturization of analytical benchtop procedures into the micro-scale provides significant advantages in regards to reaction time, cost, and integration ...

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

Increasing evidence supports transcellular propagation of toxic protein aggregates, or proteopathic seeds, as a mechanism for the initiation and progression of pathology in several neurodegenerative diseases, including Alzheimer's disease and the related tauopathies. The potentially critical role of tau seeds in disease progression strongly supports the need for a sensitive assay that readily detects seeding activity in biological samples. 
By combining the specificity of fluorescence resonance energy transfer (FRET), the sensitivity of flow cytometry, and the stability of a monoclonal cell line, an ultra-sensitive seeding assay has been engineered and is compatible with seed detection from recombinant or biological samples, including human and mouse brain homogenates. The assay employs monoclonal HEK 293T cells that stably express the aggregation-prone repeat domain (RD) of tau harboring the disease-associated P301S mutation fused to either CFP or YFP, which produce a FRET signal upon protein aggregation. The uptake of proteopathic tau seeds (but not other proteins) into the biosensor cells stimulates aggregation of RD-CFP and RD-YFP, and flow cytometry sensitively and quantitatively monitors this aggregation-induced FRET. The assay detects femtomolar concentrations (monomer equivalent) of recombinant tau seeds, has a dynamic range spanning three orders of magnitude, and is compatible with brain homogenates from tauopathy transgenic mice and human tauopathy subjects. With slight modifications, the assay can also detect seeding activity of other proteopathic seeds, such as &#945;-synuclein, and is also compatible with primary neuronal cultures. The ease, sensitivity, and broad applicability of FRET flow cytometry makes it useful to study a wide range of protein aggregation disorders.

Cell-to-cell transfer of protein aggregates, or proteopathic seeds, may underlie the progression of pathology in neurodegenerative diseases. Here, a novel FRET flow cytometry assay is described that enables specific and sensitive detection of seeding activity from recombinant or biological samples.

كشف حساسية Proteopathic البذر آخر مع الحنق التدفق الخلوي

Increasing evidence supports transcellular propagation of toxic protein aggregates, or proteopathic seeds, as a mechanism for the initiation and ...

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

The vast majority of mass spectrometry (MS)-based protein analysis methods involve an enzymatic digestion step prior to detection, typically with trypsin. This step is necessary for the generation of small molecular weight peptides, generally with MW &#60; 3,000-4,000 Da, that fall within the effective scan range of mass spectrometry instrumentation. Conventional protocols involve O/N enzymatic digestion at 37 &#186;C. Recent advances have led to the development of a variety of strategies, typically involving the use of a microreactor with immobilized enzymes or of a range of complementary physical processes that reduce the time necessary for proteolytic digestion to a few minutes (e.g., microwave or high-pressure). In this work, we describe a simple and cost-effective approach that can be implemented in any laboratory for achieving fast enzymatic digestion of a protein. The protein (or protein mixture) is adsorbed on C18-bonded reversed-phase high performance liquid chromatography (HPLC) silica particles preloaded in a capillary column, and trypsin in aqueous buffer is infused over the particles for a short period of time. To enable on-line MS detection, the tryptic peptides are eluted with a solvent system with increased organic content directly in the MS ion source. This approach avoids the use of high-priced immobilized enzyme particles and does not necessitate any aid for completing the process. Protein digestion and complete sample analysis can be accomplished in less than ~3 min and ~30 min, respectively.

A quick protocol for proteolytic digestion with an in-house built flow-through tryptic microreactor coupled to an electrospray ionization (ESI) mass spectrometer is presented. The fabrication of the microreactor, the experimental setup and the data acquisition process are described.

سريع المعالجة الأنزيمية من البروتينات للكشف عن مرض التصلب العصبي المتعدد مع Microreactor التدفق من خلال

The vast majority of mass spectrometry (MS)-based protein analysis methods involve an enzymatic digestion step prior to detection, typically with trypsin. ...

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic processing of biological samples typically involves differential manipulations of suspended particles under various force fields in order to spatially fractionate the sample based on a biological property of interest. For the resultant spatial distribution to be used as the assay readout, microfluidic devices are often subjected to microscopic analysis requiring complex instrumentation with higher cost and reduced portability. To address this limitation, we have developed an integrated electronic sensing technology for multiplexed detection of particles at different locations on a microfluidic chip. Our technology, called Microfluidic CODES, combines Resistive Pulse Sensing with Code Division Multiple Access to compress 2D spatial information into a 1D electrical signal. In this paper, we present a practical demonstration of the Microfluidic CODES technology to detect and size cultured cancer cells distributed over multiple microfluidic channels. As validated by the high-speed microscopy, our technology can accurately analyze dense cell populations all electronically without the need for an external instrument. As such, the Microfluidic CODES can potentially enable low-cost integrated lab-on-a-chip devices that are well suited for the point-of-care testing of biological samples.

We demonstrate a microfluidic platform with an integrated surface electrode network that combines resistive pulse sensing (RPS) with code division multiple access (CDMA), to multiplex detection and sizing of particles in multiple microfluidic channels.

منصة ميكروفلويديك مع تعدد كشف الالكترونية لتتبع المكانية من الجسيمات

Microfluidic processing of biological samples typically involves differential manipulations of suspended particles under various force fields in order to ...

Ovarian Cancer Detection Using Photoacoustic Flow Cytometry

Many studies suggest that the enumeration of circulating tumor cells (CTCs) may show promise as a prognostic tool for ovarian cancer. Current strategies for the detection of CTCs include flow cytometry, microfluidic devices, and real-time polymerase chain reaction (RT-PCR). Despite recent advances, methods for the detection of early ovarian cancer metastasis still lack the sensitivity and specificity required for clinical translation. Here, a novel method is presented for the detection of ovarian circulating tumor cells by photoacoustic flow cytometry (PAFC) utilizing a custom three dimensional (3D) printed system, including a flow chamber and syringe pump. This method utilizes folic acid-capped copper sulfide nanoparticles (FA-CuS NPs) to target SKOV-3 ovarian cancer cells by PAFC. This work demonstrates the affinity of these contrast agents for ovarian cancer cells. The results show NP characterization, PAFC detection, and NP uptake by fluorescence microscopy, thus demonstrating the potential of this novel system to detect ovarian CTCs at physiologically relevant concentrations.

A protocol is presented to detect circulating ovarian tumor cells utilizing a custom-made photoacoustic flow system and targeted folic acid-capped copper sulfide nanoparticles.

الكشف عن سرطان المبيض باستخدام الضوئية تدفق الصوتية الخلوية

Many studies suggest that the enumeration of circulating tumor cells (CTCs) may show promise as a prognostic tool for ovarian cancer. Current strategies ...

Design and Analysis for Fall Detection System Simplification

This paper presents a methodology based on multimodal sensors to configure a simple, comfortable and fast fall detection and human activity recognition system that can be easily implemented and adopted. The methodology is based on the configuration of specific types of sensors, machine-learning methods and procedures. The protocol is divided into four phases: (1) database creation (2) data analysis (3) system simplification and (4) evaluation. Using this methodology, we created a multimodal database for fall detection and human activity recognition, namely UP-Fall Detection. It comprises data samples from 17 subjects that perform 5 types of falls and 6 different simple activities, during 3 trials. All information was gathered using 5 wearable sensors (tri-axis accelerometer, gyroscope and light intensity), 1 electroencephalograph helmet, 6 infrared sensors as ambient sensors, and 2 cameras in lateral and front viewpoints. The proposed novel methodology adds some important stages to perform a deep analysis of the following design issues in order to simplify a fall detection system: a) select which sensors or combination of sensors are to be used in a simple fall detection system, b) determine the best placement of the sources of information, and c) select the most suitable machine learning classification method for fall and human activity detection and recognition. Even though some multimodal approaches reported in literature only focus on one or two of the above-mentioned issues, our methodology allows simultaneously solving these three design problems related to a human fall and activity detection and recognition system.

We present a methodology based on multimodal sensors to configure a simple, comfortable and fast fall detection and human activity recognition system. The goal is to build a system for accurate fall detection that can be easily implemented and adopted.

تصميم وتحليل لتبسيط نظام الكشف عن السقوط

This paper presents a methodology based on multimodal sensors to configure a simple, comfortable and fast fall detection and human activity recognition ...

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Current therapeutic innovations, such as CAR-T cell therapy, are heavily reliant on viral-mediated gene delivery. Although efficient, this technique is accompanied by high manufacturing costs, which has brought about an interest in using alternative methods for gene delivery. Electroporation is an electro-physical, non-viral approach for the intracellular delivery of genes and other exogenous materials. Upon the application of an electric field, the cell membrane temporarily allows molecular delivery into the cell. Typically, electroporation is performed on the macroscale to process large numbers of cells. However, this approach requires extensive empirical protocol development, which is costly when working with primary and difficult-to-transfect cell types. Lengthy protocol development, coupled with the requirement of large voltages to achieve sufficient electric-field strengths to permeabilize the cells, has led to the development of micro-scale electroporation devices. These micro-electroporation devices are manufactured using common microfabrication techniques and allow for greater experimental control with the potential to maintain high throughput capabilities. This work builds off a microfluidic-electroporation technology capable of detecting the level of cell membrane permeabilization at a single-cell level under continuous flow. However, this technology was limited to 4 cells processed per second, and thus a new approach for increasing the system throughput is proposed and presented here. This new technique, denoted as cell-population-based feedback control, considers the cell permeabilization response to a variety of electroporation pulsing conditions and determines the best-suited electroporation pulse conditions for the cell type under test. A higher-throughput mode is then used, where this 'optimal' pulse is applied to the cell suspension in transit. The steps for fabricating the device, setting up and running the microfluidic experiments, and analyzing the results are presented in detail. Finally, this micro-electroporation technology is demonstrated by delivering a DNA plasmid encoding for green fluorescent protein (GFP) into HEK293 cells.

This protocol describes the microfabrication techniques required to build a lab-on-a-chip, microfluidic electroporation device. The experimental setup performs controlled, single-cell-level transfections in a continuous flow and can be extended to higher throughputs with population-based control. An analysis is provided showcasing the ability to electrically monitor the degree of cell membrane permeabilization in real-time.

تصنيع وتشغيل التدفق المستمر ، نظام التثقيب الكهربائي الدقيق مع كشف النفاذية

Current therapeutic innovations, such as CAR-T cell therapy, are heavily reliant on viral-mediated gene delivery. Although efficient, this technique is ...

برنامج الذكاء الاصطناعي للكشف عن داء المثقبيات وتصنيفه

Superior Auto-Identification of Trypanosome Parasites by Using a Hybrid Deep-Learning Model

Trypanosomiasis is a significant public health problem in several regions across the world, including South Asia and Southeast Asia. The identification of hotspot areas under active surveillance is a fundamental procedure for controlling disease transmission. Microscopic examination is a commonly used diagnostic method. It is, nevertheless, primarily reliant on skilled and experienced personnel. To address this issue, an artificial intelligence (AI) program was introduced that makes use of a hybrid deep learning technique of object identification and object classification neural network backbones on the in-house low-code AI platform (CiRA CORE). The program can identify and classify the protozoan trypanosome species, namely Trypanosoma cruzi, T. brucei, and T. evansi, from oil-immersion microscopic images. The AI program utilizes pattern recognition to observe and analyze multiple protozoa within a single blood sample and highlights the nucleus and kinetoplast of each parasite as specific characteristic features using an attention map.
To assess the AI program&#39;s performance, two unique modules are created that provide a variety of statistical measures such as accuracy, recall, specificity, precision, F1 score, misclassification rate, receiver operating characteristics (ROC) curves, and precision versus recall (PR) curves. The assessment findings show that the AI algorithm is effective at identifying and categorizing parasites. By delivering a speedy, automated, and accurate screening tool, this technology has the potential to transform disease surveillance and control. It could also assist local officials in making more informed decisions on disease transmission-blocking strategies.

تسليط الضوء على المؤلف: الكشف عن أنواع المثقبيات التي يحركها الذكاء الاصطناعي من الصور المجهرية 

Worldwide medical blood parasites were automatically screened using simple steps on a low-code AI platform. The prospective diagnosis of blood films was improved by using an object detection and classification method in a hybrid deep learning model. The collaboration of active monitoring and well-trained models helps to identify hotspots of trypanosome transmission.

التعرف التلقائي الفائق على طفيليات المثقبيات باستخدام نموذج التعلم العميق الهجين

Trypanosomiasis is a significant public health problem in several regions across the world, including South Asia and Southeast Asia. The identification of ...