ونحن ممتنون للمختبر الوطني شمال غرب المحيط الهادئ (بننل) النووية عملية العلم مبادرة (نبسي)-صندوق دعم "المختبرات الموجهة للبحث" والتنمية (لدرد). الدكتور سايانديف تشاترجي قدم الجسيمات المركبة بويهميتي. الوصول الآلي قدمت من خلال "اقتراح المستخدم العام جورج ر. إيلي البيئية الجزيئي علوم المختبرات" (امسل). امسل منشأة مستخدم علمية وطنية برعاية "مكتب البيولوجية" والبحوث البيئية (البر) في بننل. وتتولى بننل Battelle للكيان التشغيلي المعين تحت العقد دي-AC05-76RL01830.

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وزارة شؤون المرأة تقنية قوية في توصيف السطحية للمواد العضوية وغير العضوية على مستوى النانو (nm) مع ارتفاع القرار1. على سبيل المثال، فإنه يستخدم على نطاق واسع لتحليل العينات الصلبة والجافة مثل المواد الجيولوجية26 وأشباه الموصلات27. ومع ذلك، فقد القيود ف?...

المسح الإلكتروني المجهري (SEM) طبق على نطاق واسع للتحقيق في مجموعة متنوعة من العينات بإنتاج الصور عالية الدقة1. مطيافية الأشعة السينية المشتتة الطاقة (EDX) المرتبطة بوزارة شؤون المرأة يتيح تحديد تكوين عنصري1. تقليديا، يتم تطبيق وزارة شؤون المرأة لتصوير العينات الجافة والصلبة فقط. في السنوات الثلاثين الأخيرة، وضعت SEM البيئي (اسيم) لتحليل العينات المائية جزئية في بخار بيئة2،3،،من45. ومع ذلك، اسيم غير قادر على الصور عينات الرطب، والسوائل تماما مع الدقة العالية المطلوبة6. كما تم تطوير الرطب SEM الخلايا لعينات صورة الرطب استخدام SEM7،8؛ ومع ذلك، هذه الخلايا وقد وضعت أساسا للعينات البيولوجية المستطار تصوير إلكترون ومتاحة أكثر للتطبيقات مع تلك التصاميم9،10.
للتصدي للتحديات في تحليل العينات المختلفة في بيئتها الأصلية السائل باستخدام SEM، نحن اخترع جهاز فراغ موائع جزيئية متوافقة، ونظام للتحليل في السائل الفراغ واجهة (سالفي)، لتمكين عالية الدقة المكانية الثانوية إلكترون (SE) التصوير وعنصري تحليل العينات السائلة استخدام وضع فراغ عالية في sem. يتضمن هذا الأسلوب رواية الميزات الفريدة التالية: 1) السائل هو سبر مباشرة في فتحه صغيرة من 1-2 ميكرومتر في القطر؛ 2) السائل هو عقد داخل الحفرة بالتوتر السطحي؛ و 3) سالفي المحمولة ويمكن تكييفها لأكثر من منصة تحليلية11،،من1213،14،15،16،17 ،18.
سالفي يتكون من 100 نانومتر السيليكون السميك نتريد (سين) غشاء و microchannel واسعة 200 ميكرون مصنوعة من كتلة بولي دايمثيل سيلوكسان (PDMS). يتم تطبيق الإطار غشاء الخطيئة لختم microchannel. تفاصيل تصنيع واعتبارات التصميم الرئيسية فصلت في الورقات السابقة والبراءات11،،من1920. حاليا، اشترت الشركة المصنعة الرائدة، وموزع لتوريد مستهلكات للفحص المجهري رخصة لبيع الأجهزة سالفي تجارياً للسائل SEM التطبيقات21،22.
لقد ثبت تطبيقات سالفي في أدوات تحليلية تستند إلى الفراغ باستخدام مجموعة متنوعة من المحاليل والمخاليط السائل المعقدة، بما في ذلك الأغشية الحيوية وخلايا الثدييات وجسيمات نانوية وقطب المواد12، 14 , 17 , 20 , 23 , 24-ومع ذلك، تستخدم معظم الأعمال المذكورة آنفا وقت الطيران الثانوية أيون الطيف الكتلي (ToF-سيمز) كأداة التحليل الرئيسية، ومن ثم تطبيق سائل وزارة شؤون المرأة مع سالفي لم تستكشف تماما. وقد استخدمت في هذا العمل، سالفي لدراسة الجسيمات الغروية غير كروية الأكبر حجماً في السائل باستخدام سائل SEM تصوير وتحليل عنصري EDX. العينة تتكون من جسيمات الوه توليفها في المختبر. الحجم سوبميكروميتير boehmite جزيئات معروفة موجودة في النفايات المشعة عالية المستوى في موقع هانفورد. وهي تذوب ببطء وقد يسبب مشاكل انسيابية في معالجة النفايات. ولذلك، من المهم أن تمتلك القدرة على توصيف الجسيمات بويهميتي في السائل25. يمكن استخدام هذا النهج التقني لدراسة بويهميتي في مختلف الظروف الفيزيائية لتحسين فهم هذه الجسيمات وخصائص انسيابية ذات الصلة. وقد استخدمت هذه الجسيمات خطوة بخطوة لشرح كيفية تطبيق سالفي للتفريغ العالي ووزارة شؤون المرأة ومن أجل دراسة الجسيمات معلقة في السائل. هي أبرز النقاط الفنية الرئيسية لاندماج سالفي ووزارة شؤون المرأة ووزارة شؤون المرأة الحصول على البيانات داخل الورقة.
وينص البروتوكول على مظاهرة لتحليل عينة السائل باستخدام سالفي والتصوير سائلة بوزارة شؤون المرأة، لأولئك الذين يرغبون في استخدام هذه التقنية الجديدة في تطبيقات متنوعة للسائل ووزارة شؤون المرأة في المستقبل.

<table><tbody><tr><td>Carbon Coater</td><td>Cressington</td><td>208 Carbon</td><td>It is accompanied with thickness monitor MTM-10.</td></tr><tr><td>SEM</td><td>FEI</td><td>Quanta 3D FEG</td><td>It provides highly resolved scanning electron microscopy and elemental analysis.</td></tr><tr><td>System for Analysis at the Liquid Vacuum Interface (SALVI)</td><td>Pacific Northwest National Laboratory</td><td>N/A</td><td>SALVI is a unique, vacuum compatible microfluidic cell that enables the characterization of the liquid sample using vacuu- based scientific instrument.</td></tr><tr><td>PEEK Union</td><td>Valco</td><td>ZU1TPK</td><td>The polyether ether ketone union is used for connecting the inlet and outlet of SALVI</td></tr><tr><td>Syringe</td><td>BD</td><td>309659</td><td>1 mL</td></tr><tr><td>Pipette</td><td>Thermo Fisher Scientific</td><td>21-377-821</td><td>Range: 100 to 1,000 mL</td></tr><tr><td>Pipette Tip 1</td><td>Neptune</td><td>2112.96.BS</td><td>1,000 &amp;micro;L</td></tr><tr><td>Pipette Tip 2</td><td>Rainin</td><td>17001865</td><td>20 &amp;micro;L</td></tr><tr><td>Syringe Pump</td><td>Harvard Apparatus</td><td>70-2213</td><td>It is used to inject the liquid sample into the SALVI device.</td></tr><tr><td>pH meter</td><td>Fisher Scientific/accumet</td><td>13-636-AP72</td><td>It is used for measuring the pH of AlOOH in DI water.</td></tr><tr><td>Barnstead Ultrapure Water System, UV/UF</td><td>Thermo Scientific Barnstead</td><td>Nanopure diamond D11931</td><td>It is used for producing DI water.</td></tr><tr><td>Centrifuge tubes</td><td>Fisher scientific/Falcon</td><td>15-527-90</td><td>15 mL</td></tr><tr><td>Bransonic ultrasonic cleaner</td><td>Sigma-Aldrich</td><td>2510</td><td>It is used to ultrasonicate the AlOOH liquid sample.</td></tr><tr><td>Balance</td><td>Mettler Toledo</td><td>11106015</td><td>XS64</td></tr><tr><td>AlOOH</td><td>Pacific Northwest National Laboratory</td><td>N/A</td><td>It is synthesized by scientists at Pacific Northwest National Laboratory.</td></tr><tr><td>xT microscope Control</td><td>FEI</td><td>Quanta 3D FEG</td><td>Default microscope control software of SEM Quanta 3D FEG</td></tr><tr><td>EDAX Genesis software</td><td>EDAX</td><td>N/A</td><td>The software is used for collecting the EDX elemental information of the samples.</td></tr><tr><td>Teflon tubing</td><td>SUPELCO</td><td>58697-U</td><td>It is used for introducing the sample into the microchannel and holding adequate volume of liquid.</td></tr></tbody></table>

in situ characterization of boehmite particles in water using liquid sem

ويتحقق في الموقع التصوير وعنصري تحليل الجزيئات بويهميتي (الوه) في المياه باستخدام النظام للتحليل في واجهة فراغ السائل (سالفي) والمسح الضوئي المجهر الإلكتروني (SEM). وتصف هذه الورقة الأسلوب والخطوات الرئيسية في دمج الفراغ سافلي متوافق مع وزارة شؤون المرأة والحصول على الإلكترونات الثانوية صور (جنوب شرق) من الجزيئات في السائل في فراغ عالية. مطيافية الأشعة السينية المشتتة الطاقة (EDX) يستخدم للحصول على تحليل عنصري للجسيمات في العينات السائلة والتحكم بما في ذلك المياه (DI) فقط وقناة فارغة، وكذلك. المركب بويهميتي (الووه) جسيمات معلقة في سائل تستخدم كنموذج في التوضيح SEM السائل. تظهر النتائج أن الجسيمات يمكن تصويرها في وضع سراج الدين مع قرار جيد (أي400 نانومتر). يظهر الطيف EDX الوه إشارة هامة من الألومنيوم (Al) بالمقارنة مع المياه دي ومراقبة قناة فارغة. في الوضع الطبيعي السائل SEM تقنية قوية لدراسة الجزيئات في السائل مع العديد من التطبيقات المثيرة. هذا الإجراء يهدف إلى توفير الدراية التقنية بغية إجراء التصوير سائلة بوزارة شؤون المرأة وتحليل EDX استخدام سالفي وتقليل المخاطر المحتملة عند استخدام هذا النهج.

وترد نتائج تمثيلية لإظهار كيف يتم تصويرها الجسيمات وتحليلها باستخدام في الموقع تصوير SEM السائل إلى جانب EDX. وتشمل النتائج SE الصور والأطياف EDX. تم الحصول على صور سراج الدين في 100000 X ومستويات التكبير X 200,000 في الشكل 1. يصور الشكل 1a سراج الدي...

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In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

في الموقع توصيف الجسيمات بويهميتي في المياه باستخدام SEM السائل

نقدم إجراء التصوير في الوقت الحقيقي وتحليل تكوين عنصري لجسيمات بويهميتي في المياه بالسائل في الموقع الميكروسكوب الإلكتروني المسح.

In situ imaging and elemental analysis of boehmite (AlOOH) particles in water is realized using the System for Analysis at the Liquid Vacuum Interface ...

in-situ-characterization-boehmite-particles-water-using-liquid

Research

JoVE Journal

Chemistry

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

9.1K Views.  Pacific Northwest National Laboratory. نقدم إجراء التصوير في الوقت الحقيقي وتحليل تكوين عنصري لجسيمات بويهميتي في المياه بالسائل في الموقع الميكروسكوب الإلكتروني المسح.

Video: In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Simulation of the Planetary Interior Differentiation Processes in the Laboratory

A planetary interior is under high-pressure and high-temperature conditions and it has a layered structure. There are two important processes that led to that layered structure, (1) percolation of liquid metal in a solid silicate matrix by planet differentiation, and (2) inner core crystallization by subsequent planet cooling. We conduct high-pressure and high-temperature experiments to simulate both processes in the laboratory. Formation of percolative planetary core depends on the efficiency of melt percolation, which is controlled by the dihedral (wetting) angle. The percolation simulation includes heating the sample at high pressure to a target temperature at which iron-sulfur alloy is molten while the silicate remains solid, and then determining the true dihedral angle to evaluate the style of liquid migration in a crystalline matrix by 3D visualization. The 3D volume rendering is achieved by slicing the recovered sample with a focused ion beam (FIB) and taking SEM image of each slice with a FIB/SEM crossbeam instrument. The second set of experiments is designed to understand the inner core crystallization and element distribution between the liquid outer core and solid inner core by determining the melting temperature and element partitioning at high pressure. The melting experiments are conducted in the multi-anvil apparatus up to 27 GPa and extended to higher pressure in the diamond-anvil cell with laser-heating. We have developed techniques to recover small heated samples by precision FIB milling and obtain high-resolution images of the laser-heated spot that show melting texture at high pressure. By analyzing the chemical compositions of the coexisting liquid and solid phases, we precisely determine the liquidus curve, providing necessary data to understand the inner core crystallization process.

The high-pressure and high-temperature experiments described here mimic planet interior differentiation processes. The processes are visualized and better understood by high-resolution 3D imaging and quantitative chemical analysis.

محاكاة لعمليات الكواكب الداخلية التمايز في المختبر

A planetary interior is under high-pressure and high-temperature conditions and it has a layered structure. There are two important processes that led to ...

Engineering

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Element diffusion rates and temperature/pressure control a range of fundamental volcanic and metamorphic processes. Such processes are often recorded in lamellae exsolved from host mineral phases. Thus, the analysis of the orientation, size, morphology, composition and spacing of exsolution lamellae is an area of active research in the geosciences. The conventional study of these lamellae has been conducted by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and more recently with focused ion beam (FIB)-based nanotomography, yet with limited chemical information. Here, we explore the use of atom probe tomography (APT) for the nanoscale analysis of ilmenite exsolution lamellae in igneous titanomagnetite from ash deposits erupted from the active Soufri&#232;re Hills Volcano (Montserrat, British West Indies). APT allows the precise calculation of interlamellar spacings (14&#8211;29 &#177; 2 nm) and reveals smooth diffusion profiles with no sharp phase boundaries during the exchange of Fe and Ti/O between the exsolved lamellae and the host crystal. Our results suggest that this novel approach permits nanoscale measurements of lamellae composition and interlamellar spacing that may provide a means to estimate the lava dome temperatures necessary to model extrusion rates and lava dome failure, both of which play a key role in volcanic hazard mitigation efforts.

Analysis of the morphology, composition, and spacing of exsolution lamellae can provide essential information to understand geological processes related to volcanism and metamorphism. We present a novel application of APT for the characterization of such lamellae and compare this approach to the conventional use of electron microscopy and FIB-based nanotomography.

تحليل التصوير المقطعي الذري للمراحل المعدنية الexsolvedه

Element diffusion rates and temperature/pressure control a range of fundamental volcanic and metamorphic processes. Such processes are often recorded in ...

Environment

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory

Given their potential for significant property improvements relative to their large grained counterparts, much work has been devoted to the continued development of nanocrystalline metals. Despite these efforts, the transition of these materials from the lab bench to actual applications has been blocked by the inability to produce large scale parts that retain the desired nanocrystalline microstructures. Following the development of a method proven to stabilize the nanosized grain structure to temperatures approaching that of the melting point for the given metal, the US Army Research Laboratory (ARL) has progressed to the next stage in the development of these materials - namely the production of large scale parts suitable for testing and evaluation in a range of relevant test environments. This report provides a broad overview of the ongoing efforts in the processing, characterization, and consolidation of these materials at ARL. In particular, focus is placed on the methodology used for producing the nanocrystalline metal powders, in both small and large-scale amounts, that are at the center of ongoing research efforts.

This paper provides a brief overview of the ongoing efforts at the Army Research Laboratory on the processing of bulk nanocrystalline metals with an emphasis on the methodologies used for the production of the novel metal powders.

تجهيز المعادن خوصات الأكبر في مختبر أبحاث الجيش الأمريكي

Given their potential for significant property improvements relative to their large grained counterparts, much work has been devoted to the continued ...

Determination of Aggregate Surface Morphology at the Interfacial Transition Zone (ITZ)

Here, we present a comprehensive method to illustrate the uneven distribution of the interfacial transition zone (ITZ) around the aggregate and the effect of aggregate surface morphology on the formation of ITZ. First, a model concrete sample is prepared with a spherical ceramic particle in roughly the central part of the cement matrix, acting as a coarse aggregate used in common concrete/mortar. After curing until the designed age, the sample is scanned by X-ray computed tomography to determine the relative location of the ceramic particle inside the cement matrix. Three locations of the ITZ are chosen: above the aggregate, on the side of the aggregate, and below the aggregate. After a series of treatments, the samples are scanned with a SEM-BSE detector. The resultant images were further processed using a digital image processing method (DIP) to obtain quantitative characteristics of the ITZ. The surface morphology is characterized at the pixel level based on the digital image. Thereafter, K-means clustering method is used to illustrate the effect of surface roughness on ITZ formation.

Determination of Aggregate Surface Morphology at the Interfacial Transition Zone ITZ

Hereby, we proposed a protocol to illustrate the effect of aggregate surface morphology on the ITZ microstructure. The SEM-BSE image were quantitatively analyzed to obtain ITZ's porosity gradient via digital image processing and a K-means clustering algorithm was further employed to establish a relationship between porosity gradient and surface roughness.

تحديد المورفولوجية السطحية التجميعية في المنطقة الانتقالية بين الوجهين (ITZ)

Here, we present a comprehensive method to illustrate the uneven distribution of the interfacial transition zone (ITZ) around the aggregate and the effect ...

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy

Physical and chemical processes at solid-liquid interfaces play a crucial role in many natural and technological phenomena, including catalysis, solar energy and fuel generation, and electrochemical energy storage. Nanoscale characterization of such interfaces has recently been achieved using cryogenic electron microscopy, thereby providing a new path to advancing our fundamental understanding of interface processes.
This contribution provides a practical guide to mapping the structure and chemistry of solid-liquid interfaces in materials and devices using an integrated cryogenic electron microscopy approach. In this approach, we pair cryogenic sample preparation which allows stabilization of solid-liquid interfaces with cryogenic focused ion beam (cryo-FIB) milling to create cross-sections through these complex buried structures. Cryogenic scanning electron microscopy (cryo-SEM) techniques performed in a dual-beam FIB/SEM enable direct imaging as well as chemical mapping at the nanoscale. We discuss practical challenges, strategies to overcome them, as well as protocols for obtaining optimal results. While we focus in our discussion on interfaces in energy storage devices, the methods outlined are broadly applicable to a range of fields where solid-liquid interface play a key role.

Cryogenic Focused Ion Beam (FIB) and Scanning Electron Microscopy (SEM) techniques can provide key insights into the chemistry and morphology of intact solid-liquid interfaces. Methods for preparing high quality Energy Dispersive X-ray (EDX) spectroscopic maps of such interfaces are detailed, with a focus on energy storage devices.

توصيف المقياس النانوي للواجهات السائلة الصلبة عن طريق اقتران طحن الحزمة الأيونية المركزة على التبريد مع المجهر الإلكتروني الماسح والتحليل الطيفي

Physical and chemical processes at solid-liquid interfaces play a crucial role in many natural and technological phenomena, including catalysis, solar ...

<meta charset="utf8"></head><body>التحليل متعدد الوسائط لتكوين العظام باستخدام نماذج الزرع

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

The extensive characterization of tissue mineralization in the context of bone regeneration represents a significant challenge, given the numerous modalities that are currently available for analysis. Here, we propose a workflow for a comprehensive evaluation of new bone formation using a relevant large animal osseous ex vivo explant. A bone defect (diameter = 3.75 mm; depth = 5.0 mm) is created in an explanted sheep femoral head and injected with a macroporous bone substitute loaded with a pro-osteogenic growth factor (bone morphogenetic protein 2 - BMP2). Subsequently, the explant is maintained in culture for a 28-day period, allowing cellular colonization and subsequent bone formation. To evaluate the quality and structure of newly mineralized tissue, the following successive methods are set up: (i) Characterization and high-resolution 3D images of the entire explant using micro-CT, followed by deep learning image analyses to enhance the discrimination of mineralized tissues; (ii) Nano-indentation to determine the mechanical properties of the newly formed tissue; (iii) Histological examinations, such as Hematoxylin/Eosin/Saffron (HES), Goldner's trichrome, and Movat's pentachrome to provide a qualitative assessment of mineralized tissue, particularly with regard to the visualization of the osteoid barrier and the presence of bone cells; (iv) Back-scattering scanning electron microscopy (SEM) mapping with internal reference to quantify the degree of mineralization and provide detailed insights into surface morphology, mineral composition, and bone-biomaterial interface; (v) Raman spectroscopy to characterize the molecular composition of the mineralized matrix and to provide insights into the persistence of BMP2 within the cement through the detection of peptide bonds. This multimodal analysis will provide an effective assessment of newly formed bone and comprehensive qualitative and quantitative insights into mineralized tissues. Through the standardization of these protocols, we aim to facilitate interstudy comparisons and improve the validity and reliability of research findings.

<meta charset="utf8"></head><body>أضواء الكاتب: التقنيات المتقدمة لتوصيف تمعدن الأنسجة في أبحاث تجديد العظام

The proposed protocol entails a global approach to assess bone formation in the context of bone regeneration using multimodal analyses. It aims to provide qualitative and quantitative information on new bone formation, enhancing the rigor and validity of basic and pre-clinical investigations.

التوصيف الشامل لتمعدن الأنسجة في نموذج Ex Vivo

The extensive characterization of tissue mineralization in the context of bone regeneration represents a significant challenge, given the numerous ...

Bioengineering

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Characterizing the first event of biological production of calcium carbonate requires a combination of microscopy approaches. First, intracellular pH distribution and calcium ions can be observed using live microscopy over time. This allows identification of the life stage and the tissue with the feature of interest for further electron microscopy studies. Life stage and tissues of interest are typically higher in pH and Ca signals. 
Here, using H. elegans, we present a protocol to characterize the presence of calcium carbonate structures in a biological specimen on the scanning electron microscope (SEM), using energy-dispersive X-ray spectroscopy (EDS) to visualize elemental composition, using electron backscatter diffraction (EBSD) to determine the presence of crystalline structures, and using transmission electron microscopy (TEM) to analyze the composition and structure of the material. In this protocol, a focused ion beam (FIB) is used to isolate samples with dimension suitable for TEM analysis. As FIB is a site specific technique, we demonstrate how information from the previous techniques can be used to identify the region of interest, where Ca signals are highest.

We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.

توصيف تكلس الأحداث عن طريق لايف البصرية والمجهر الإلكتروني تقنيات في Tubeworm البحرية

Characterizing the first event of biological production of calcium carbonate requires a combination of microscopy approaches. First, intracellular pH ...

Biology

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Membrane remodeling occurs constantly at the plasma membrane and within cellular organelles. To fully dissect the role of the environment (ionic conditions, protein and lipid compositions, membrane curvature) and the different partners associated with specific membrane reshaping processes, we undertake in vitro bottom-up approaches. In recent years, there has been keen interest in revealing the role of septin proteins associated with major diseases. Septins are essential and ubiquitous cytoskeletal proteins that interact with the plasma membrane. They are implicated in cell division, cell motility, neuro-morphogenesis, and spermiogenesis, among other functions. It is, therefore, important to understand how septins interact and organize at membranes to subsequently induce membrane deformations and how they can be sensitive to specific membrane curvatures. This article aims to decipher the interplay between the ultra-structure of septins at a molecular level and the membrane remodeling occurring at a micron scale. To this end, budding yeast, and mammalian septin complexes were recombinantly expressed and purified. A combination of in vitro assays was then used to analyze the self-assembly of septins at the membrane. Supported lipid bilayers (SLBs), giant unilamellar vesicles (GUVs), large unilamellar vesicles (LUVs), and wavy substrates were used to study the interplay between septin self-assembly, membrane reshaping, and membrane curvature.

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Septins are cytoskeletal proteins. They interact with lipid membranes and can sense but also generate membrane curvature at the micron scale. We describe in this protocol bottom-up in vitro methodologies for analyzing membrane deformations, curvature-sensitive septin binding, and septin filament ultrastructure.

في الموقع توصيف الجسيمات بويهميتي في المياه باستخدام SEM السائل

Summary

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Chapters in this video

محاكاة لعمليات الكواكب الداخلية التمايز في المختبر

تحليل التصوير المقطعي الذري للمراحل المعدنية الexsolvedه

تجهيز المعادن خوصات الأكبر في مختبر أبحاث الجيش الأمريكي

تحديد المورفولوجية السطحية التجميعية في المنطقة الانتقالية بين الوجهين (ITZ)

توصيف المقياس النانوي للواجهات السائلة الصلبة عن طريق اقتران طحن الحزمة الأيونية المركزة على التبريد مع المجهر الإلكتروني الماسح والتحليل الطيفي

التوصيف الشامل لتمعدن الأنسجة في نموذج Ex Vivo

التوصيف الشامل لتمعدن الأنسجة في نموذج Ex Vivo

التوصيف الشامل لتمعدن الأنسجة في نموذج Ex Vivo

توصيف تكلس الأحداث عن طريق لايف البصرية والمجهر الإلكتروني تقنيات في Tubeworm البحرية

من أسفل إلى أعلى في طرق المختبر لفحص التنظيم فوق الهيكلي ، وإعادة تشكيل الغشاء ، وسلوك حساسية الانحناء للسبتين