يشكر المؤلفون البروفيسور يو فنغ، والدكتورة جينا بريدل، وإيان وودوارد، ولوكاس عطية على مناقشاتهم المفيدة.

<ol>
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وليس لدى صاحبي البلاغ ما يكشفان عنه.

الجهاز الحالي للدولة من بين الفن لاختبار الأدوية الرئوية لجرعة استنشاق كاملة هو Impactor مولد المقبل (NGI)، الذي يقيس القطر الأيرودينامية من الهباء الجوي4. ثم يتم استخدام بيانات التحجيم هذه للتنبؤ بتوليد الرئة الذي سيودع فيه الهباء الجوي استنادا إلى ارتباط تم تطويره لذكر بالغ سليم...

العديد من الأمراض الرئوية مثل سرطان الرئة ومرض الانسداد الرئوي المزمن (COPD) تظهر اختلافات إقليمية في خصائص المرض؛ ومع ذلك ، هناك نقص في التقنيات العلاجية المتاحة لاستهداف تسليم الأدوية إلى المناطق المريضة فقط من الرئة1. وقد أظهرت نماذج متعددة ديناميكية السوائل الحسابية (CFD) أنه من الممكن تعديل ملامح ترسب المخدرات عن طريق تحديد يبسط محددة في الرئة2,3. يجري تطوير كل من أجهزة الاستنشاق ومحولات أنبوب الرغامى (ET) ذات قدرات الاستهداف الإقليمية في مختبرنا للسيطرة على توزيع الهباء الجوي على مناطق الرئة المريضة. توسيع نطاق هذه المبادئ للاستخدام السريري محدود بقدرة الاختبار الحالية قبل السريرية. ومن المعروف أن الموقع الدقيق لرواسب الدواء داخل الرئة هو أفضل مؤشر على الفعالية. ومع ذلك ، فإن التقييمات الصيدلانية الحالية للعلاجات القابلة للاستنشاق يتم التنبؤ بها في معظم الأحيان باستخدام الارتباطات في الجسم الحي في المختبر لحجم الجسيمات إلى مجرد ترسب تقريبي4. لا تسمح هذه التقنية بأي تحليل مكاني لتحديد آثار هندسة مجرى الهواء المختلفة على التوزيع الإقليمي من خلال الفصوص المختلفة للرئة. بالإضافة إلى ذلك ، يفتقر هذا الاختبار إلى هندسة الرئة الدقيقة تشريحيا ، والتي أظهر الباحثون أنها يمكن أن يكون لها تأثير كبير على ملامح الترسب5. وقد بذلت بعض الجهود لدمج هندسة الرئة الخاصة بالمرضى في بروتوكولات الاختبار من خلال إضافة الشعب الهوائية العليا؛ ومع ذلك، فإن معظم هذه النهج عينة تسليم الهباء الجوي إلى أجيال مختلفة من الرئة بدلا من كلفصالرئة 6،7،8. يقدم البروتوكول التالي طريقة عالية الإنتاجية لتوليد نماذج الرئة الخاصة بالمرضى مع القدرة على قياس ترسب الجسيمات النسبية في كل من الفصوص الخمسة للرئة9.
يتم إنشاء الرئتين نموذج دقيق تشريحيا من قبل 3D الطباعة المريض التصوير المقطعي المحوسب (CT) بمسح. عند استخدامها جنبا إلى جنب مع نظام تدفق تجميعها بسهولة، يمكن التحكم في معدلات التدفق النسبي من خلال كل من فصوص الرئة نموذج مستقل ومصممة لمحاكاة تلك التركيبة السكانية المريض مختلفة و / أو حالات المرض. مع هذه الطريقة، يمكن للباحثين اختبار فعالية الأساليب العلاجية المحتملة في هندسة الرئة ذات الصلة وربط أداء كل طريقة مع تطور مورفولوجيا المريضة. هنا، يتم اختبار تصميمين للأجهزة تم تطويرهما في مختبرنا لقدرتهما على زيادة الترسب في فص الرئة المطلوب من خلال التحكم في موقع إطلاق الهباء الجوي في الفم أو القصبة الهوائية. كما أن هذا البروتوكول لديه القدرة على التأثير بشكل كبير على تطوير إجراءات شخصية للمرضى من خلال تسهيل التنبؤ السريع بفعالية العلاج في الرئة النموذجية الخاصة ببيانات الأشعة المقطعية لهذا المريض.

<table><tbody><tr><td>1/4&quot; Plastic Barbed Tube Fitting</td><td>McMaster Carr</td><td>5372K111</td><td></td></tr><tr><td>10 um Filter Paper</td><td>Fisher</td><td>1093-110</td><td></td></tr><tr><td>1um Fluorescent Polystyrene Particles</td><td>Polysciences</td><td>15702-10</td><td></td></tr><tr><td>1um Non-Fluorescent Polystyrene Particles</td><td>Polysciences</td><td>8226</td><td></td></tr><tr><td>2-Propanol</td><td>Fisher</td><td>A516-4</td><td>Referred to in protocol as &quot;IPA&quot;</td></tr><tr><td>3/8&quot; Plastic Barbed Tube Fitting</td><td>McMaster Carr</td><td>5372K117</td><td></td></tr><tr><td>Air Flow Meter (1 - 280 mL/min)</td><td>McMaster Carr</td><td>41695K32</td><td>Referred to in protocol as &quot;flow meter&quot;</td></tr><tr><td>Carbon M1 3D Printer</td><td>Carbon 3D</td><td></td><td>https://www.carbon3d.com/, Associated software referred to in protocol as &quot;slicing software&quot;</td></tr><tr><td>Collison Jet Nebulizer</td><td>CH Technologies</td><td>ARGCNB0008 (CN-25)</td><td>6 Jet MRE style horizontal collision with glass jar, Referred to in protocol as &quot;nebulizer&quot;, http://chtechusa.com/Manuals/MRE_Collison_Manual.pdf</td></tr><tr><td>Convection Oven</td><td>Yamato</td><td>DKN602</td><td></td></tr><tr><td>Copley Critical Flow Controller TPK2000 Reve 120V</td><td>MSP Corp</td><td>0001-01-9810</td><td>Referred to in protocol as &quot;flow controller&quot;</td></tr><tr><td>Copley High Capacity Pump Model HCP5</td><td>MSP Corp</td><td>0001-01-9982</td><td>Referred to in protocol as &quot;vacuum pump&quot;</td></tr><tr><td>Cytation</td><td>BioTek</td><td>CYT5MPV</td><td>Multifunctional Spectrophotometer/Fluorescent imager equiped with 4x/20x/40x objectives and DAPI/GFP/TexasRed laser/filter cubes</td></tr><tr><td>EPU40 Resin</td><td>Carbon 3D</td><td></td><td>https://www.carbon3d.com/materials/epu-elastomeric-polyurethane/, Referred to in protocol as &quot;soft resin&quot;</td></tr><tr><td>Filter for vacuum pump</td><td>Whatman</td><td>6722-5000</td><td></td></tr><tr><td>Flow Meter Model DFM 2000</td><td>MSP Corp</td><td>0001-01-8764</td><td>Referred to in protocol as &quot;electronic flow meter&quot;</td></tr><tr><td>ImageJ Software</td><td>ImageJ</td><td></td><td>https://imagej.nih.gov/ij/download.html</td></tr><tr><td>Inline Air Flow Control Valve (Push-to-Connect)</td><td>McMaster Carr</td><td>62005K333</td><td>Referred to in protocol as &quot;valve&quot;</td></tr><tr><td>Inline Filter Devices</td><td>Whatman</td><td>WHA67225000</td><td></td></tr><tr><td>Marine-Grade Plywood Sheet</td><td>McMaster Carr</td><td>62005K333</td><td>Referred to in protocol as &quot;wooden board&quot;</td></tr><tr><td>Materialise Mimics Software</td><td>Materialise</td><td></td><td>https://www.materialise.com/en/medical/mimics-innovation-suite, Referred to in protocol as &quot;CT scan software&quot;</td></tr><tr><td>Meshmixer Software</td><td>Autodesk</td><td></td><td>http://www.meshmixer.com/, Referred to in protocol as &quot;mesh editing software&quot;</td></tr><tr><td>Methanol</td><td>Fisher</td><td>A454-4</td><td></td></tr><tr><td>Opticure LED Cube</td><td>APM Technica</td><td>102843</td><td>Referred to in protocol as &quot;UV oven&quot;</td></tr><tr><td>PR25 Resin</td><td>Carbon 3D</td><td></td><td>https://www.carbon3d.com/materials/uma-urethanemethacrylate, /Referred to in protocol as &quot;hard resin&quot;</td></tr><tr><td>PVC Tube for Chemicals</td><td>McMaster Carr</td><td>5231K161</td><td>1/4&quot; ID</td></tr><tr><td>Screws</td><td></td><td></td><td></td></tr><tr><td>SolidWorks Software</td><td>Dassault Syst&amp;egrave;mes&amp;nbsp;SolidWorks Corporation</td><td></td><td>https://www.solidworks.com/, Referred to in protocol as &quot;3D modeling software&quot;</td></tr><tr><td>Straight Flow Rectangular Manifold</td><td>McMaster Carr</td><td>1125T31</td><td></td></tr><tr><td>Tubing to Flow Controller</td><td>McMaster Carr</td><td>5233K65</td><td>3/8&quot; ID</td></tr><tr><td>Wire</td><td></td><td></td><td></td></tr></tbody></table>

evaluating regional pulmonary deposition using patient-specific 3d printed lung models

يحد من تطوير العلاجات المستهدفة للأمراض الرئوية توافر طرق الاختبار قبل السريرية مع القدرة على التنبؤ بتسليم الهباء الجوي الإقليمي. الاستفادة من الطباعة ثلاثية الأبعاد لتوليد نماذج الرئة المريض محددة، ونحن الخطوط العريضة لتصميم عالية الإنتاجية، والإعداد التجريبي في المختبر لتحديد ترسب رئوي كروي. يتم إجراء هذا النظام مع مزيج من المكونات المطبوعة المتاحة تجاريا و3D ويسمح لمعدل التدفق من خلال كل فص من الرئة إلى أن تسيطر بشكل مستقل. يتم قياس تسليم الهباء الجوي الفلوري إلى كل فص باستخدام المجهر الفلوري. هذا البروتوكول لديه القدرة على تعزيز نمو الطب الشخصي لأمراض الجهاز التنفسي من خلال قدرته على نموذج مجموعة واسعة من التركيبة السكانية للمرضى وحالات المرض. يمكن تعديل كل من هندسة نموذج الرئة المطبوع ثلاثي الأبعاد وإعداد ملف تعريف تدفق الهواء بسهولة لتعكس البيانات السريرية للمرضى الذين يعانون من اختلاف العمر والعرق والجنس. يمكن دمج أجهزة توصيل الأدوية ذات الصلة سريريا ، مثل أنبوب القصبة الهوائية الموضح هنا ، في إعداد الاختبار للتنبؤ بشكل أكثر دقة بقدرة الجهاز على استهداف التسليم العلاجي لمنطقة مريضة من الرئة. تسمح براعة هذا الإعداد التجريبي بتخصيصه ليعكس العديد من ظروف الاستنشاق ، مما يعزز صرامة الاختبار العلاجي قبل السريري.

تتبع الجسيمات في نطاق الحجم هذا (1-5 ميكرومتر) وظروف التدفق (1-10 لتر / دقيقة) خطوط تيار السوائل استنادا إلى كل من عدد ستوكس النظري وبيانات الجسم الحي؛ لذلك ، في حالة عدم وجود جهاز تسليم مستهدف ، من المتوقع أن تترسب الجسيمات المنبعثة في نموذج الرئة وفقا للنسبة المئوية لإجمالي تدفق الهواء المحول...

Watch this Scientific Journal Video about Evaluating Regional Pulmonary Deposition using Patient-Specific 3D Printed Lung Models at JoVE.com

Evaluating Regional Pulmonary Deposition using Patient-Specific 3D Printed Lung Models

نقدم طريقة عالية الإنتاجية في المختبر لتحديد ترسب الرئة الإقليمي على مستوى الفص باستخدام نماذج الرئة المطبوعة ثلاثية الأبعاد المشتقة من الأشعة المقطعية مع ملامح تدفق الهواء غير القادرة.

تقييم الترسب الرئوي الإقليمي باستخدام نماذج الرئة المطبوعة ثلاثية الأبعاد الخاصة بالمرضى

Development of targeted therapies for pulmonary diseases is limited by the availability of preclinical testing methods with the ability to predict ...

evaluating-regional-pulmonary-deposition-using-patient-specific-3d

Research

JoVE Journal

Bioengineering

4.2K Views.  University of Delaware. نقدم طريقة عالية الإنتاجية في المختبر لتحديد ترسب الرئة الإقليمي على مستوى الفص باستخدام نماذج الرئة المطبوعة ثلاثية الأبعاد المشتقة من الأشعة المقطعية مع ملامح تدفق الهواء غير القادرة.

Video: Evaluating Regional Pulmonary Deposition using Patient-Specific 3D Printed Lung Models

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

In the present study, we combined an in vitro 3D lung tumor model with an in silico model to optimize predictions of drug response based on a specific mutational background. The model is generated on a decellularized porcine scaffold that reproduces tissue-specific characteristics regarding extracellular matrix composition and architecture including the basement membrane. We standardized a protocol that allows artificial tumor tissue generation within 14 days including three days of drug treatment. Our article provides several detailed descriptions of 3D read-out screening techniques like the determination of the proliferation index Ki67 staining&#39;s, apoptosis from supernatants by M30-ELISA and assessment of epithelial to mesenchymal transition (EMT), which are helpful tools for evaluating the effectiveness of therapeutic compounds. We could show compared to 2D culture a reduction of proliferation in our 3D tumor model that is related to the clinical situation. Despite of this lower proliferation, the model predicted EGFR-targeted drug responses correctly according to the biomarker status as shown by comparison of the lung carcinoma cell lines HCC827 (EGFR -mutated, KRAS wild-type) and A549 (EGFR wild-type, KRAS-mutated) treated with the tyrosine-kinase inhibitor (TKI) gefitinib. To investigate drug responses of more advanced tumor cells, we induced EMT by long-term treatment with TGF-beta-1 as assessed by vimentin/pan-cytokeratin immunofluorescence staining. A flow-bioreactor was employed to adjust culture to physiological conditions, which improved tissue generation. Furthermore, we show the integration of drug responses upon gefitinib treatment or TGF-beta-1 stimulation - apoptosis, proliferation index and EMT - into a Boolean in silico model. Additionally, we explain how drug responses of tumor cells with a specific mutational background and counterstrategies against resistance can be predicted. We are confident that our 3D in vitro approach especially with its in silico expansion provides an additional value for preclinical drug testing in more realistic conditions than in 2D cell culture.

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

We present a three-dimensional (3D) lung cancer model based on a biological collagen scaffold to study sensitivity towards non-small-cell-lung-cancer-(NSCLC)-targeted therapies. We demonstrate different read-out techniques to determine the proliferation index, apoptosis and epithelial-mesenchymal transition (EMT) status. Collected data are integrated into an in silico model for prediction of drug sensitivity.

والأنسجة المشتركة 3D هندسيا<em&gt; في المختبر</em&gt; /<em&gt; في السيليكو</em&gt; الرئة نموذج الورم للتنبؤ فعالية الدواء في خلفيات الطفرات محددة

In the present study, we combined an in vitro 3D lung tumor model with an in silico model to optimize predictions of drug response based on a specific ...

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

Tuberculosis (TB) still holds a major threat to the health of people worldwide, and there is a need for cost-efficient but reliable models to help us understand the disease mechanisms and advance the discoveries of new treatment options. In vitro cell cultures of monolayers or co-cultures lack the three-dimensional (3D) environment and tissue responses. Herein, we describe an innovative in vitro model of a human lung tissue, which holds promise to be an effective tool for studying the complex events that occur during infection with Mycobacterium tuberculosis (M. tuberculosis). The 3D tissue model consists of tissue-specific epithelial cells and fibroblasts, which are cultured in a matrix of collagen on top of a porous membrane. Upon air exposure, the epithelial cells stratify and secrete mucus at the apical side. By introducing human primary macrophages infected with M. tuberculosis to the tissue model, we have shown that immune cells migrate into the infected-tissue and form early stages of TB granuloma. These structures recapitulate the distinct feature of human TB, the granuloma, which is fundamentally different or not commonly observed in widely used experimental animal models. This organotypic culture method enables the 3D visualization and robust quantitative analysis that provides pivotal information on spatial and temporal features of host cell-pathogen interactions. Taken together, the lung tissue model provides a physiologically relevant tissue micro-environment for studies on TB. Thus, the lung tissue model has potential implications for both basic mechanistic and applied studies. Importantly, the model allows addition or manipulation of individual cell types, which thereby widens its use for modelling a variety of infectious diseases that affect the lungs.

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.

نموذج الأنسجة 3D الرئة الإنسان للدراسات وظيفية على<em&gt; المتفطرة السلية</em&gt; العدوى

Tuberculosis (TB) still holds a major threat to the health of people worldwide, and there is a need for cost-efficient but reliable models to help us ...

Immunology and Infection

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Human lung development and disease has been difficult to study due to the lack of biologically relevant in vitro model systems. Human induced pluripotent stem cells (hiPSCs) can be differentiated stepwise into 3D multicellular lung organoids, made of both epithelial and mesenchymal cell populations. We recapitulate embryonic developmental cues by temporally introducing a variety of growth factors and small molecules to efficiently generate definitive endoderm, anterior foregut endoderm, and subsequently lung progenitor cells. These cells are then embedded in growth factor reduced (GFR)-basement membrane matrix medium, allowing them to spontaneously develop into 3D lung organoids in response to external growth factors. These whole lung organoids (WLO) undergo early lung developmental stages including branching morphogenesis and maturation after exposure to dexamethasone, cyclic AMP and isobutylxanthine. WLOs possess airway epithelial cells expressing the markers KRT5 (basal), SCGB3A2 (club) and MUC5AC (goblet) as well as alveolar epithelial cells expressing HOPX (alveolar type I) and SP-C (alveolar type II). Mesenchymal cells are also present, including smooth muscle actin (SMA), and platelet-derived growth factor receptor A (PDGFR&#945;). iPSC derived WLOs can be maintained in 3D culture conditions for many months and can be sorted for surface markers to purify a specific cell population. iPSC derived WLOs can also be utilized to study human lung development, including signaling between the lung epithelium and mesenchyme, to model genetic mutations on human lung cell function and development, and to determine the cytotoxicity of infective agents.

The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.

جيل من 3D الجهازيات الرئة الكاملة من الخلايا الجذعية المستحثة متعددة القدرات لنمذجة البيولوجيا التنموية الرئة والمرض

Human lung development and disease has been difficult to study due to the lack of biologically relevant in vitro model systems. Human induced pluripotent ...

Developmental Biology

3D Printing of Preclinical X-ray Computed Tomographic Data Sets

Three-dimensional printing allows for the production of highly detailed objects through a process known as additive manufacturing. Traditional, mold-injection methods to create models or parts have several limitations, the most important of which is a difficulty in making highly complex products in a timely, cost-effective manner.1 However, gradual improvements in three-dimensional printing technology have resulted in both high-end and economy instruments that are now available for the facile production of customized models.2 These printers have the ability to extrude high-resolution objects with enough detail to accurately represent in vivo images generated from a preclinical X-ray CT scanner. With proper data collection, surface rendering, and stereolithographic editing, it is now possible and inexpensive to rapidly produce detailed skeletal and soft tissue structures from X-ray CT data. Even in the early stages of development, the anatomical models produced by three-dimensional printing appeal to both educators and researchers who can utilize the technology to improve visualization proficiency. 3, 4 The real benefits of this method result from the tangible experience a researcher can have with data that cannot be adequately conveyed through a computer screen. The translation of pre-clinical 3D data to a physical object that is an exact copy of the test subject is a powerful tool for visualization and communication, especially for relating imaging research to students, or those in other fields. Here, we provide a detailed method for printing plastic models of bone and organ structures derived from X-ray CT scans utilizing an Albira X-ray CT system in conjunction with PMOD, ImageJ, Meshlab, Netfabb, and ReplicatorG software packages.

Using modern plastic extrusion and printing technologies, it is now possible to quickly and inexpensively produce physical models of X-ray CT data taken in a laboratory. The three -dimensional printing of tomographic data is a powerful visualization, research, and educational tool that may now be accessed by the preclinical imaging community.

3D الطباعة الأشعة السينية ما قبل السريرية البيانات المحسوبة تصوير الشعاعي الطبقي مجموعات

Three-dimensional printing allows for the production of highly detailed objects through a process known as additive manufacturing.  Traditional, ...

Biology

Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling

Translation of novel discoveries to human disease is limited by the availability of human tissue-based models of disease. Precision-cut lung slices (PCLS) used as 3D lung tissue cultures (3D-LTCs) represent an elegant and biologically highly relevant 3D cell culture model, which highly resemble in situ tissue due to their complexity, biomechanics and molecular composition. Tissue slicing is widely applied in various animal models. 3D-LTCs derived from human PCLS can be used to analyze responses to novel drugs, which might further help to better understand the mechanisms and functional effects of drugs in human tissue. The preparation of PCLS from surgically resected lung tissue samples of patients, who experienced lung lobectomy, increases the accessibility of diseased and peritumoral tissue. Here, we describe a detailed protocol for the generation of human PCLS from surgically resected soft-elastic patient lung tissue. Agarose was introduced into the bronchoalveolar space of the resectates, thus preserving lung structure and increasing the tissue&#39;s stiffness, which is crucial for subsequent slicing. 500 &#181;m thick slices were prepared from the tissue block with a vibratome. Biopsy punches taken from PCLS ensure comparable tissue sample sizes and further increase the amount of tissue samples. The generated lung tissue cultures can be applied in a variety of studies in human lung biology, including the pathophysiology and mechanisms of different diseases, such as fibrotic processes at its best at (sub-)cellular levels. The highest benefit of the 3D-LTC ex vivo model is its close representation of the in situ human lung in respect of 3D tissue architecture, cell type diversity and lung anatomy as well as the potential for assessment of tissue from individual patients, which is relevant to further develop novel strategies for precision medicine.

Generation of Human 3D Lung Tissue Cultures 3D-LTCs for Disease Modeling

Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.

جيل زرع الأنسجة البشرية الرئة 3D (3D-الصلب) للنمذجة المرض

Translation of novel discoveries to human disease is limited by the availability of human tissue-based models of disease. Precision-cut lung slices (PCLS) ...

Medicine

Development and Evaluation of 3D-Printed Cardiovascular Phantoms for Interventional Planning and Training

Catheter-based interventions are standard treatment options for cardiovascular pathologies. Therefore, patient-specific models could help training physicians&#39; wire-skills as well as improving planning of interventional procedures. The aim of this study was to develop a manufacturing process of patient-specific 3D-printed models for cardiovascular interventions.
To create a 3D-printed elastic phantom, different 3D-printing materials were compared to porcine biological tissues (i.e., aortic tissue) in terms of mechanical characteristics. A fitting material was selected based on comparative tensile tests and specific material thicknesses were defined. Anonymized contrast-enhanced CT-datasets were collected retrospectively. Patient-specific volumetric models were extracted from these datasets and subsequently 3D-printed. A pulsatile flow loop was constructed to simulate the intraluminal blood flow during interventions. Models&#39; suitability for clinical imaging was assessed by x-ray imaging, CT, 4D-MRI and (Doppler) ultrasonography. Contrast medium was used to enhance visibility in x-ray-based imaging. Different catheterization techniques were applied to evaluate the 3D-printed phantoms in physicians&#39; training as well as for pre-interventional therapy planning.
Printed models showed a high printing resolution (~30 &#181;m) and mechanical properties of the chosen material were comparable to physiological biomechanics. Physical and digital models showed high anatomical accuracy when compared to the underlying radiological dataset. Printed models were suitable for ultrasonic imaging as well as standard x-rays. Doppler ultrasonography and 4D-MRI displayed flow patterns and landmark characteristics (i.e., turbulence, wall shear stress) matching native data. In a catheter-based laboratory setting, patient-specific phantoms were easy to catheterize. Therapy planning and training of interventional procedures on challenging anatomies (e.g., congenital heart disease (CHD)) was possible.
Flexible patient-specific cardiovascular phantoms were 3D-printed, and the application of common clinical imaging techniques was possible. This new process is ideal as a training tool for catheter-based (electrophysiological) interventions and can be used in patient-specific therapy planning.

Here we present development of a mock circulation setup for multimodal therapy evaluation, pre-interventional planning, and physician-training on cardiovascular anatomies. With the application of patient-specific tomographic scans, this setup is ideal for therapeutic approaches, training, and education in individualized medicine.

تطوير وتقييم الأشباح القلبية الوعائية المطبوعة ثلاثية الأبعاد للتخطيط والتدريب التدخلي

Catheter-based interventions are standard treatment options for cardiovascular pathologies. Therefore, patient-specific models could help training ...

A Personalized 3D-Printed Model for Preoperative Evaluation in Thyroid Surgery

The anatomic structure of the surgical area of thyroid cancer is complex. It is very important to comprehensively and carefully evaluate the tumor location and its relation with the capsule, trachea, esophagus, nerves, and blood vessels before operation. This paper introduces an innovative 3D-printed model establishment method based on computerized tomography (CT) DICOM images. We established a personalized 3D-printed model of the cervical thyroid surgery field for each patient who needed thyroid surgery to help clinicians evaluate the key points and difficulties of the surgery and select the operation methods of key parts as a basis. The results showed that this model is conducive to preoperative discussion and the formulation of operation strategies. In particular, as a result of the clear display of the recurrent laryngeal nerve and parathyroid gland locations in the thyroid operation field, injury to them can be avoided during surgery, the difficulty of thyroid surgery reduced, and the incidence of postoperative hypoparathyroidism and complications related to recurrent laryngeal nerve injury reduced too. Moreover, this 3D-printed model is intuitive and aids communication for the signing of informed consent by patients before surgery.

Here, a new method of establishing a personalized 3D-printed model for preoperative evaluation of thyroid surgery is proposed. It is conducive to preoperative discussion, reducing the difficulty of thyroid surgery.

نموذج شخصي مطبوع 3D للتقييم قبل الجراحة في جراحة الغدة الدرقية

The anatomic structure of the surgical area of thyroid cancer is complex. It is very important to comprehensively and carefully evaluate the tumor ...

3D إعادة الإعمار لتشخيص وعلاج العقيدات الرئوية

A 3D Digital Model for the Diagnosis and Treatment of Pulmonary Nodules

The three-dimensional (3D) reconstruction of pulmonary nodules using medical images has introduced new technical approaches for diagnosing and treating pulmonary nodules, and these approaches are progressively being acknowledged and adopted by physicians and patients. Nonetheless, constructing a relatively universal 3D digital model of pulmonary nodules for diagnosis and treatment is challenging due to device differences, shooting times, and nodule types. The objective of this study is to propose a new 3D digital model of pulmonary nodules that serves as a bridge between physicians and patients and is also a cutting-edge tool for pre-diagnosis and prognostic evaluation. Many AI-driven pulmonary nodule detection and recognition methods employ deep learning techniques to capture the radiological features of pulmonary nodules, and these methods can achieve a good area under-the-curve (AUC) performance. However, false positives and false negatives remain a challenge for radiologists and clinicians. The interpretation and expression of features from the perspective of pulmonary nodule classification and examination are still unsatisfactory. In this study, a method of continuous 3D reconstruction of the whole lung in horizontal and coronal positions is proposed by combining existing medical image processing technologies. Compared with other applicable methods, this method allows users to rapidly locate pulmonary nodules and identify their fundamental properties while also observing pulmonary nodules from multiple perspectives, thereby providing a more effective clinical tool for diagnosing and treating pulmonary nodules.

تسليط الضوء على المؤلف: نموذج رقمي 3D لتشخيص وعلاج العقيدات الرئوية  

The objective of this study is to develop a novel 3D digital model of pulmonary nodules that serves as a communication bridge between physicians and patients and is also a cutting-edge tool for pre-diagnosis and prognostic evaluation.

نموذج رقمي 3D لتشخيص وعلاج العقيدات الرئوية

The three-dimensional (3D) reconstruction of pulmonary nodules using medical images has introduced new technical approaches for diagnosing and treating ...

تقييم الترسب الرئوي الإقليمي باستخدام نماذج الرئة المطبوعة ثلاثية الأبعاد الخاصة بالمرضى

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والأنسجة المشتركة 3D هندسيا

نموذج الأنسجة 3D الرئة الإنسان للدراسات وظيفية على

جيل من 3D الجهازيات الرئة الكاملة من الخلايا الجذعية المستحثة متعددة القدرات لنمذجة البيولوجيا التنموية الرئة والمرض

3D الطباعة الأشعة السينية ما قبل السريرية البيانات المحسوبة تصوير الشعاعي الطبقي مجموعات

جيل زرع الأنسجة البشرية الرئة 3D (3D-الصلب) للنمذجة المرض

تطوير وتقييم الأشباح القلبية الوعائية المطبوعة ثلاثية الأبعاد للتخطيط والتدريب التدخلي

نموذج شخصي مطبوع 3D للتقييم قبل الجراحة في جراحة الغدة الدرقية

نموذج رقمي 3D لتشخيص وعلاج العقيدات الرئوية

نموذج رقمي 3D لتشخيص وعلاج العقيدات الرئوية

نموذج رقمي 3D لتشخيص وعلاج العقيدات الرئوية