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التأثيرات الخافضة للضغط ل Huotan Jiedu Tongluo في الفئران المصابة بارتفاع ضغط الدم من النوع H

<ol>
	<li>Noone, C., Dwyer, C. P., Murphy, J., Newell, J., Molloy, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparative+effectiveness+of+physical+activity+interventions+and+antihypertensive+pharmacological+interventions+in+reducing+blood+pressure+in+people+with+hypertension:+Protocol+for+a+systematic+review+and+network+meta-analysis.">Comparative effectiveness of physical activity interventions and antihypertensive pharmacological interventions in reducing blood pressure in people with hypertension: Protocol for a systematic review and network meta-analysis.</a> Syst Rev. 7 (1), 128 (2018).</li><li>Huang, K., Zhang, Z., Huang, S., Jia, Y., Zhang, M., Yun, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+association+between+retinal+vessel+abnormalities+and+h-type+hypertension.">The association between retinal vessel abnormalities and h-type hypertension.</a> BMC Neurol. 21 (1), 6 (2021).</li><li>Tan, Y., Nie, F., Wu, G., Guo, F., Wang, Y., Wang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+h-type+hypertension+on+intraplaque+neovascularization+assessed+by+contrast-enhanced+ultrasound.">Impact of h-type hypertension on intraplaque neovascularization assessed by contrast-enhanced ultrasound.</a> J Atheroscler Thromb. 29 (4), 492-501 (2022).</li><li>Towfighi, A., Markovic, D., Ovbiagele, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pronounced+association+of+elevated+serum+homocysteine+with+stroke+in+subgroups+of+individuals:+A+nationwide+study.">Pronounced association of elevated serum homocysteine with stroke in subgroups of individuals: A nationwide study.</a> J Neurol Sci. 298 (1-2), 153-157 (2010).</li><li>Zhong, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+homocysteine+and+blood+pressure+related+to+poor+outcome+of+acute+ischemia+stroke+in+Chinese+population.">High homocysteine and blood pressure related to poor outcome of acute ischemia stroke in Chinese population.</a> Plos One. 9 (9), e107498 (2014).</li><li>Hao, P., Jiang, F., Cheng, J., Ma, L., Zhang, Y., Zhao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traditional+Chinese+medicine+for+cardiovascular+disease:+Evidence+and+potential+mechanisms.">Traditional Chinese medicine for cardiovascular disease: Evidence and potential mechanisms.</a> J Am Coll Cardiol. 69 (24), 2952-2966 (2017).</li><li>Tian, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Huotan+Jiedu+Tongluo+decoction+inhibits+balloon-injury-induced+carotid+artery+intimal+hyperplasia+in+the+rat+through+the+perk-eif2&#945;-atf4+pathway+and+autophagy+mediation.">Huotan Jiedu Tongluo decoction inhibits balloon-injury-induced carotid artery intimal hyperplasia in the rat through the perk-eif2&#945;-atf4 pathway and autophagy mediation.</a> Evid Based Complement Alternat Med. 2021, 5536237 (2021).</li><li>Simko, F., Pechanova, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Potential+roles+of+melatonin+and+chronotherapy+among+the+new+trends+in+hypertension+treatment.">Potential roles of melatonin and chronotherapy among the new trends in hypertension treatment.</a> J Pineal Res. 47 (2), 127-133 (2009).</li><li>Li, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=H-type+hypertension+is+a+risk+factor+for+cerebral+small-vessel+disease.">H-type hypertension is a risk factor for cerebral small-vessel disease.</a> BioMed Res Int. 2020, 6498903 (2020).</li><li>Rodrigo, R., Passalacqua, W., Araya, J., Orellana, M., Rivera, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homocysteine+and+essential+hypertension.">Homocysteine and essential hypertension.</a> J Clin Pharmacol. 43 (12), 1299-1306 (2003).</li><li>Lehmann, M., Gottfries, C. G., Regland, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+cognitive+impairment+in+the+elderly:+Homocysteine+is+an+early+marker.">Identification of cognitive impairment in the elderly: Homocysteine is an early marker.</a> Dement Geriatr Cogn. 10 (1), 12-20 (1999).</li><li>dos Santos, E. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evidence+that+folic+acid+deficiency+is+a+major+determinant+of+hyperhomocysteinemia+in+Parkinson's+disease.">Evidence that folic acid deficiency is a major determinant of hyperhomocysteinemia in Parkinson's disease.</a> Metab Brain Dis. 24 (2), 257-269 (2009).</li><li>Zhao, W., Gao, F., Lv, L., Chen, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+interaction+of+hypertension+and+homocysteine+increases+the+risk+of+mortality+among+middle-aged+and+older+population+in+the+United+States.">The interaction of hypertension and homocysteine increases the risk of mortality among middle-aged and older population in the United States.</a> J Hypertens. 40 (2), 254-263 (2022).</li><li>Woo, K. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hyperhomocyst(e)inemia+is+a+risk+factor+for+arterial+endothelial+dysfunction+in+humans.">Hyperhomocyst(e)inemia is a risk factor for arterial endothelial dysfunction in humans.</a> Circulation. 96 (8), 2542-2544 (1997).</li><li>Lu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+intervention+of+enalapril+maleate+and+folic+acid+tablet+on+the+expressions+of+the+grp78+and+chop+and+vascular+remodeling+in+the+vascular+smooth+muscle+cells+of+h-hypertensive+rats+with+homocysteine.">The intervention of enalapril maleate and folic acid tablet on the expressions of the grp78 and chop and vascular remodeling in the vascular smooth muscle cells of h-hypertensive rats with homocysteine.</a> Eur Rev Med Pharmaco. 22 (7), 2160-2168 (2018).</li><li>L&#243;pez-Garc&#237;a, P., Moreira, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+forces+for+the+origin+of+the+eukaryotic+nucleus.">Selective forces for the origin of the eukaryotic nucleus.</a> BioEssays. 28 (5), 525-533 (2006).</li><li>Minamino, T., Komuro, I., Kitakaze, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endoplasmic+reticulum+stress+as+a+therapeutic+target+in+cardiovascular+disease.">Endoplasmic reticulum stress as a therapeutic target in cardiovascular disease.</a> Circ Res. 107 (9), 1071-1082 (2010).</li><li>Chen, X., Cubillos-Ruiz, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endoplasmic+reticulum+stress+signals+in+the+tumour+and+its+microenvironment.">Endoplasmic reticulum stress signals in the tumour and its microenvironment.</a> Nat Rev Cancer. 21 (2), 71-88 (2021).</li><li>Ji, C., Kaplowitz, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hyperhomocysteinemia,+endoplasmic+reticulum+stress,+and+alcoholic+liver+injury.">Hyperhomocysteinemia, endoplasmic reticulum stress, and alcoholic liver injury.</a> World J Gastroentero. 10 (12), 1699-1708 (2004).</li><li>Zhang, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homocysteine+induces+programmed+cell+death+in+human+vascular+endothelial+cells+through+activation+of+the+unfolded+protein+response.">Homocysteine induces programmed cell death in human vascular endothelial cells through activation of the unfolded protein response.</a> J Biol Chem. 276 (38), 35867-35874 (2001).</li><li>Cunard, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endoplasmic+reticulum+stress,+a+driver+or+an+innocent+bystander+in+endothelial+dysfunction+associated+with+hypertension.">Endoplasmic reticulum stress, a driver or an innocent bystander in endothelial dysfunction associated with hypertension.</a> Cur Hypertens Rep. 19 (8), 64 (2017).</li><li>Casas, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=GRP78+at+the+centre+of+the+stage+in+cancer+and+neuroprotection.">GRP78 at the centre of the stage in cancer and neuroprotection.</a> Front Neurosci. 11, 177 (2017).</li><li>Urano, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coupling+of+stress+in+the+ER+to+activation+of+JNK+protein+kinases+by+transmembrane+protein+kinase+ire1.">Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase ire1.</a> Science. 287 (5453), 664-666 (2000).</li><li>Borghi, A., Verstrepen, L., Beyaert, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TRAF2+multitasking+in+tnf+receptor-induced+signaling+to+nf-&#954;b,+map+kinases+and+cell+death.">TRAF2 multitasking in tnf receptor-induced signaling to nf-&#954;b, map kinases and cell death.</a> Biochem Pharmacol. 116, 1-10 (2016).</li><li>Wen, X. -. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Butylphthalide+suppresses+neuronal+cells+apoptosis+and+inhibits+JNK-caspase3+signaling+pathway+after+brain+ischemia+/reperfusion+in+rats.">Butylphthalide suppresses neuronal cells apoptosis and inhibits JNK-caspase3 signaling pathway after brain ischemia /reperfusion in rats.</a> Cell Mol Neurobiol. 36 (7), 1087-1095 (2016).</li><li>Jin, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serine-threonine+protein+kinase+activation+may+be+an+effective+target+for+reducing+neuronal+apoptosis+after+spinal+cord+injury.">Serine-threonine protein kinase activation may be an effective target for reducing neuronal apoptosis after spinal cord injury.</a> Neural Regen Res. 10 (11), 1830-1835 (2015).</li><li>Xu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrogen+and+propofol+combination+therapy+inhibits+endoplasmic+reticulum+stress+and+remarkably+attenuates+cerebral+ischemia-reperfusion+injury+and+ogd+injury+in+hippocampus.">Estrogen and propofol combination therapy inhibits endoplasmic reticulum stress and remarkably attenuates cerebral ischemia-reperfusion injury and ogd injury in hippocampus.</a> Biomed Pharmacother. 108, 1596-1606 (2018).</li></ol>

نعلن أن البحث قد أجري في غياب أي علاقات تجارية أو مالية يمكن تفسيرها على أنها تضارب محتمل في المصالح.

ارتفاع ضغط الدم هو واحد من أكثر اضطرابات القلب والأوعية الدموية شيوعا التي تؤثر على ثلث السكان البالغين وتزيد من خطر الإصابة بالسكتة الدماغية وأمراض القلب التاجية وفشل القلب والكلى8. ارتفاع ضغط الدم من النوع H هو نوع خاص من ارتفاع ضغط الدم يشير إلى تزامن ارتفاع ضغط الدم الأساس...

أصبح ارتفاع ضغط الدم ، وهو عامل خطر رئيسي للنوبات القلبية والسكتة الدماغية والفشل الكلوي ، تحديا كبيرا للصحة العامة يؤثر على مليار شخص في جميع أنحاءالعالم 1. الهوموسيستين (Hcy) ، وهو حمض أميني يحتوي على مجموعة ثيول ، هو وسيط استقلابي حيوي لاستقلاب الميثيونين. يتم تعريف ارتفاع ضغط الدم مع ارتفاع مستويات Hcy في البلازما على أنه ارتفاع ضغط الدم من النوع H ، والذي يمكن أن يكون عامل خطر كبير لحدوث وتكرار أمراض القلب والأوعية الدموية الدماغية مثل السكتة الدماغية 2,3. أفادت الدراسات الحديثة أن الإقامة المشتركة لارتفاع ضغط الدم من النوع H يمكن أن تؤدي إلى تفاقم الآثار الجانبية للأمراض القلبية الوعائية والدماغية4. والجدير بالذكر أن 75٪ من المرضى في الصين الذين يعانون من ارتفاع ضغط الدم من النوع H يعانون من ارتفاع ضغط الدم الأساسي ، مما يؤثر بشكل خطير على نوعية الحياة5. في الوقت الحاضر ، يشمل علاج ارتفاع ضغط الدم من النوع H بشكل رئيسي الطب الغربي. ومع ذلك ، قد يسبب بعض الآثار الضارة وضعف الامتثال ولم يعد بإمكانه تلبية احتياجات الإدارة الشاملة لارتفاع ضغط الدم من النوع H.
الطب الصيني التقليدي (TCM) هو مورد فريد له تاريخ يزيد عن 2000 عام في الصين. نظرا للحاجة غير الملباة للسيطرة على ارتفاع ضغط الدم في الطب الغربي ، بدأ الأطباء في النظر في الدور المحتمل للطب الصيني التقليدي في الوقاية والعلاج من ارتفاع ضغط الدم من النوعH 6. Huotan Jiedu Tongluo Decoction (HTJDTLD) هي صيغة الطب الصيني التقليدي التي صاغها البروفيسور يو دينغ ، مستمدا من خبرته السريرية الواسعة7. على مدار أكثر من 20 عاما من التطبيق السريري ، أظهر HTJDTLD فعالية ملحوظة في علاج أمراض القلب والأوعية الدموية والدماغية1. ومع ذلك ، لم يتم الإبلاغ عما إذا كان HTJDTLD له آثار علاجية في ارتفاع ضغط الدم من النوع H. لذلك ، كنا نهدف إلى استكشاف التأثيرات الخافضة للضغط والآليات المحددة ل HTJDTLD في الفئران المصابة بارتفاع ضغط الدم من النوع H وتحديد الأدوية العلاجية المحتملة لعلاج ارتفاع ضغط الدم من النوع H.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1st Strand cDNA Synthesis SuperMix for qPCR</td>
			<td>Yeasen,China</td>
			<td>11149ES</td>
			<td>cDNA synthesis kit&#160;</td>
		</tr>
		<tr>
			<td>Anti-beta-actin antibody</td>
			<td>Bioss, China</td>
			<td>bs-0061R</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-caspase-3 antibody</td>
			<td>Bioss, China</td>
			<td>bs-0081R</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-GPR78 antibody</td>
			<td>Abcam, USA</td>
			<td>ab108513</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-JNK antibody</td>
			<td>Abcam, USA</td>
			<td>ab76572</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-p-JNK antibody</td>
			<td>Bioss, China</td>
			<td>bsm-52462R</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-rabbit IgG antibody</td>
			<td>Bioss, China</td>
			<td>bs-0295G-HRP</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-TRAF2 antibody</td>
			<td>Bioss, China</td>
			<td>bs-22372R</td>
			<td></td>
		</tr>
		<tr>
			<td>Bio-Rad CFX96 Touch system&#160;</td>
			<td>Bio-Rad</td>
			<td>CFX96</td>
			<td>real-time PCR detection system&#160;</td>
		</tr>
		<tr>
			<td>ECL Western Blot Substrates</td>
			<td>Merck, MA, USA</td>
			<td>WBULP-10ML</td>
			<td></td>
		</tr>
		<tr>
			<td>Enalapril maleate folic acid tablets</td>
			<td>Yangzijiang Pharmaceutical Company, China</td>
			<td>20040991</td>
			<td></td>
		</tr>
		<tr>
			<td>FastStart SYBR Green Master</td>
			<td>Sigma</td>
			<td>FSSGMMRO</td>
			<td></td>
		</tr>
		<tr>
			<td>Fructus Trichosanthis</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Hirudo</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>intelligent noninvasive sphygmomanometer&#160;</td>
			<td>Beijing Softron Biotechnology company</td>
			<td>BP-2010A</td>
			<td></td>
		</tr>
		<tr>
			<td>Lonicerae Japonicae Flos</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Methionine</td>
			<td>Sigma, USA</td>
			<td>M9500</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix Angelicae Sinensis</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Glycyrrhizae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Nardostachyos</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Rhodiolae Crenulatae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Salviae Miltiorrhizae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix Scrophulariae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat Hcy ELISA Kits</td>
			<td>Shanghai Meimian Industrial Company, China</td>
			<td>MM-0293R2</td>
			<td></td>
		</tr>
		<tr>
			<td>RIPA buffer</td>
			<td>Shanghai Beyotime Biotechnology company</td>
			<td>P0013B</td>
			<td></td>
		</tr>
	</tbody>
</table>

the antihypertensive effects and mechanisms of huotan jiedu tongluo decoction in rats with h-type hypertension

أصبح ارتفاع ضغط الدم من النوع H ، وهو شكل محدد من ارتفاع ضغط الدم يتميز بارتفاع مستويات الحمض الاميني في البلازما (Hcy) ، تحديا كبيرا للصحة العامة في جميع أنحاء العالم. بحثت هذه الدراسة في التأثيرات الخافضة للضغط والآليات الأساسية ل Huotan Jiedu Tongluo decoction (HTJDTLD) ، وهي تركيبة طبية صينية تقليدية فعالة للغاية تستخدم عادة لعلاج تضيق الأوعية الدموية. تم استخدام الميثيونين للحث على ارتفاع ضغط الدم من النوع H في الفئران ، وتم إعطاء HTJDTLD داخل المعدة. بعد ذلك ، تم قياس ضغط الدم الانقباضي والانبساطي للشريان الذيلي للفئران بواسطة قياس الضغط الذيلي غير الباضع للفئران. تم إجراء التقييم النسيجي للشريان الأورطي عن طريق تلطيخ الهيماتوكسيلين يوزين (HE). تم استخدام مقايسة الممتز المناعي المرتبط بالإنزيم (ELISA) لقياس مستويات Hcy ، وتم استخدام تفاعل البوليميراز المتسلسل للنسخ العكسي الكمي (qRT-PCR) والنشاف الغربي لتحديد مستويات mRNA والبروتين للبروتين التنظيمي للجلوكوز 78 (GRP78) ، عامل نخر الورم (TNF) العامل المرتبط بمستقبلات 2 (TRAF2) ، كينازات c-Jun N-terminal (JNK) ، و caspase-3. أظهرت النتائج أن HTJDTLD خفض ضغط الدم بشكل كبير ، وخفف من الآفات النسيجية المرضية ، وخفض مستويات Hcy بعد العلاج بالميثيونين. علاوة على ذلك ، يثبط HTJDTLD بشكل كبير التعبير الجيني والبروتيني ل GRP78 و JNK و TRAF2 و caspase 3 ، والتي تشارك بشكل رئيسي في مسار موت الخلايا المبرمج الناجم عن الإجهاد في الشبكة الإندوبلازمية (ER). بشكل عام ، أشارت النتائج إلى أن HTJDTLD كان له تأثيرات فعالة خافضة للضغط في الفئران المصابة بارتفاع ضغط الدم من النوع H وكشفت عن الآليات الخافضة للضغط المرتبطة بتثبيط تنشيط مسار موت الخلايا المبرمج الناجم عن الإجهاد ER.

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

تسليط الضوء على المؤلف: استكشاف Huotan Jiedu Tongluo ديكوتيون كدواء خافض للضغط

الآثار الخافضة للضغط وآليات Huotan Jiedu Tongluo ديكوتيون في الفئران مع ارتفاع ضغط الدم من النوع H

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

كما هو موضح في الجدول 4 والجدول 5 ، كان ضغط الدم الانقباضي (SBP) وضغط الدم الانبساطي (DBP) أكبر بكثير في مجموعة MET من تلك الموجودة في مجموعة CON من 1 إلى 4 أسابيع. بعد علاج HTJDTLD ، كان SBP و DBP للفئران أقل بكثير من تلك الموجودة في مجموعة MET. والجدير بالذكر أن الاستخدام المشترك ل HTJDTLD و EM ك?...

Read this Scientific Article Protocol about The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension at JoVE.com

هنا ، نقدم بروتوكولا للحث على ارتفاع ضغط الدم من النوع H وتقييم الآثار الخافضة للضغط من Huotan Jiedu Tongluo ديكوتيون (HTJDTLD) تدار داخل المعدة. في الفئران المصابة بارتفاع ضغط الدم من النوع H ، كان ل HTJDTLD تأثيرات فعالة خافضة للضغط ، ربما ترتبط بتثبيط تنشيط مسار موت الخلايا المبرمج الناجم عن الإجهاد (ER).

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

أصبح ارتفاع ضغط الدم من النوع H تحديا كبيرا للصحة العامة للإنسان. يعتمد بشكل أساسي على علاج الطب الغربي ، والذي يمكن أن يكون له آثار حزينة. يستكشف بحثنا التأثيرات والآليات المضادة لارتفاع ضغط الدم ل Huotan Jiedu Tongluo decoction ، وهي صيغة طبية صينية تقليدية ، في الفئران المصابة بارتفاع ضغط الدم من النوع H.أشارت دراستنا إلى أن Huotan Jiedu Tongluo decoction كان له تأثيرات مضادة لارتفاع ضغط الدم في الفئران المصابة بارتفاع ضغط الدم من النوع H. قد تتضمن الآلية الأساسية تثبيط موت الخلايا المبرمج الناجم عن تنشيط الإجهاد ER والذي يوفر خيارا علاجيا فعالا للمرضى. سنقوم بتفجير المؤشرات الحيوية المحتملة للتشخيص المبكر والتشخيص وتطوير عقاقير علاجية جديدة لارتفاع ضغط الدم من النوع H في المستقبل.

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Research

JoVE Journal

Biochemistry

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

397 Views.  Changchun University of Chinese Medicine. أصبح ارتفاع ضغط الدم من النوع H تحديا كبيرا للصحة العامة للإنسان. يعتمد بشكل أساسي على علاج الطب الغربي ، والذي يمكن أن يكون له آثار حزينة. يستكشف بحثنا التأثيرات والآليات المضادة لارتفاع ضغط الدم ل Huotan Jiedu Tongluo decoction ، وهي صيغة طبية صينية تقليدية ، في الفئران المصابة بارتفاع ...

Video: تسليط الضوء على المؤلف: استكشاف Huotan Jiedu Tongluo ديكوتيون كدواء خافض للضغط

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health challenge worldwide. This study investigated the hypotensive effects and underlying mechanisms of Huotan Jiedu Tongluo decoction (HTJDTLD), a highly effective traditional Chinese medicine formula commonly used to treat vascular stenosis. Methionine was used to induce H-type hypertension in rats, and HTJDTLD was administered intragastrically. Then, the systolic and diastolic blood pressures of the caudal artery of rats were measured by noninvasive rat caudal manometry. Histological assessment of the aorta was performed by hematoxylin-eosin (HE) staining. Enzyme-linked immunosorbent assay (ELISA) was used to measure Hcy levels, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting were used to determine the mRNA and protein levels of Glucose regulatory protein 78 (GRP78), Tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2), c-Jun N-terminal&#160;kinases (JNK), and caspase-3. The results showed that HTJDTLD significantly lowered blood pressure, alleviated histopathological lesions, and decreased Hcy levels after methionine treatment. Moreover, HTJDTLD significantly inhibited the gene and protein expression of GRP78, JNK, TRAF2, and caspase 3, which are involved mainly in the endoplasmic reticulum (ER) stress-induced apoptosis pathway. Overall, the results indicated that HTJDTLD had effective antihypertensive effects in rats with H-type hypertension and revealed the antihypertensive mechanisms associated with inhibition of ER stress-induced apoptosis pathway activation.

Here, we present a protocol to induce H-type hypertension and evaluate the antihypertensive effects of Huotan Jiedu Tongluo decoction (HTJDTLD) administered intragastrically. In rats with H-type hypertension, HTJDTLD had effective antihypertensive effects, possibly associated with inhibition of endoplasmic reticulum (ER) stress-induced apoptosis pathway activation.

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

To begin, segregate 50 hypertensive rats into five groups, including control. Give the rats in Group 1, methionine or met group, a diet of 3%methionine for 28 days to induce H-type hypertension. For Group 2 to 4 rats, administer a combination of Huotan Jiedu Tongluo Decoction and enalapril maleate in addition to the methionine diet for 28 days.Next, create a restraining device by placing a cylindrical restraint mesh into a thermal tube and putting the thermal tube into a canvas cover. After 28 days of treatment, place a rat in the mesh. Next place the signal cable of a noninvasive sphygmomanometer under the cover.Position the rat's tail in the gap in the cover and secure it on the stabilizing form pad. Transfer the rat to a quiet measurement site 20 to 30 minutes before blood pressure measurement. Now connect the pressurized sensor's air hose connection, signal connection, and holding tube.Place the pressurization sensor at the tip of the rat's tail. A pulse wave should appear after the rat tail is inserted into the sensor. Press Start/Stop to initiate or stop the measurement.When the blood pressure test is completed, the result menu will pop up automatically. Check the average value of the measurement, standard deviation, standard, and coefficient of variation in the results menu. After euthanizing the rat, dissect it with a pair of surgical scissors and tooth forceps from the perineum to the neck.Turn over the thoracic and abdominal contents. Then navigate to the abdominal aortic bifurcation between the two kidneys. Collect the aorta up to a section of the aortic arch.The systolic and diastolic blood pressures were significantly greater in the Met Group relative to the control group from one to four weeks. The blood pressures decreased after the administration of the Huotan Jiedu Tongluo Decoction. The combined effect of the decoction and/or enalapril maleate had a stronger anti-hypertensive effect than the decoction alone.

علاج الفئران المصابة بارتفاع ضغط الدم من النوع H الناجم عن الميثيونين باستخدام Huotan Jiedu Tongluo Decoction (HTJDTLD)

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

To begin, centrifuge the coagulated blood-containing tubes at 626 to 1, 409 G for 20 minutes at two to eight degrees Celsius. Then carefully collect the supernatant in a new tube. Store the supernatant at minus 80 degrees Celsius.Next, set up blank standard and sample wells. Pipette 50 microliters of the diluted standards into the ELISA plate. Then transfer 40 microliters of the diluted samples into the sample wells.Add 10 microliters of serum into the sample wells. Now, seal the plate with a sealing film. Place the sealed plate in an incubator at 37 degrees Celsius for 30 minutes.Dilute the 30 times concentrated wash solution with distilled water to obtain a single strength wash solution. Next, remove the sealing film from the plate and discard the liquid. Now, fill each well with 200 microliters of washing solution five times.After discarding the supernatant for the last time, tap the plate dry. Pipette 50 microliters of the enzyme reagent into all wells other than the blank ones. Then seal the plate with a sealing film before placing it in an incubator at 37 degrees Celsius for 30 minutes.After washing the plate five more times, add 50 microliters of each of the color developers, A and B.Shake the plate gently to mix well. Then incubate the plate at 37 degrees Celsius at low light for 10 minutes. Now, pipette 50 microliters of the stop solution into each well to terminate the reaction.Measure the absorbance of each well sequentially at 450 nanometers. The plasma homocysteine concentration in the methionine group was twofold greater than in the control group. The plasma homocysteine concentrations were relatively lower in the treatment groups.