이 연구는 길림성 국립자연과학재단(National Natural Science Foundation of Gillin Province)의 지원을 받았다. YDZJ202301ZYTS189).

H형 고혈압 쥐에서 Huotan Jiedu Tongluo의 항고혈압 효과

<ol>
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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparative+effectiveness+of+physical+activity+interventions+and+antihypertensive+pharmacological+interventions+in+reducing+blood+pressure+in+people+with+hypertension:+Protocol+for+a+systematic+review+and+network+meta-analysis.">Comparative effectiveness of physical activity interventions and antihypertensive pharmacological interventions in reducing blood pressure in people with hypertension: Protocol for a systematic review and network meta-analysis.</a> Syst Rev. 7 (1), 128 (2018).</li><li>Huang, K., Zhang, Z., Huang, S., Jia, Y., Zhang, M., Yun, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+association+between+retinal+vessel+abnormalities+and+h-type+hypertension.">The association between retinal vessel abnormalities and h-type hypertension.</a> BMC Neurol. 21 (1), 6 (2021).</li><li>Tan, Y., Nie, F., Wu, G., Guo, F., Wang, Y., Wang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+h-type+hypertension+on+intraplaque+neovascularization+assessed+by+contrast-enhanced+ultrasound.">Impact of h-type hypertension on intraplaque neovascularization assessed by contrast-enhanced ultrasound.</a> J Atheroscler Thromb. 29 (4), 492-501 (2022).</li><li>Towfighi, A., Markovic, D., Ovbiagele, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pronounced+association+of+elevated+serum+homocysteine+with+stroke+in+subgroups+of+individuals:+A+nationwide+study.">Pronounced association of elevated serum homocysteine with stroke in subgroups of individuals: A nationwide study.</a> J Neurol Sci. 298 (1-2), 153-157 (2010).</li><li>Zhong, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+homocysteine+and+blood+pressure+related+to+poor+outcome+of+acute+ischemia+stroke+in+Chinese+population.">High homocysteine and blood pressure related to poor outcome of acute ischemia stroke in Chinese population.</a> Plos One. 9 (9), e107498 (2014).</li><li>Hao, P., Jiang, F., Cheng, J., Ma, L., Zhang, Y., Zhao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traditional+Chinese+medicine+for+cardiovascular+disease:+Evidence+and+potential+mechanisms.">Traditional Chinese medicine for cardiovascular disease: Evidence and potential mechanisms.</a> J Am Coll Cardiol. 69 (24), 2952-2966 (2017).</li><li>Tian, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Huotan+Jiedu+Tongluo+decoction+inhibits+balloon-injury-induced+carotid+artery+intimal+hyperplasia+in+the+rat+through+the+perk-eif2&#945;-atf4+pathway+and+autophagy+mediation.">Huotan Jiedu Tongluo decoction inhibits balloon-injury-induced carotid artery intimal hyperplasia in the rat through the perk-eif2&#945;-atf4 pathway and autophagy mediation.</a> Evid Based Complement Alternat Med. 2021, 5536237 (2021).</li><li>Simko, F., Pechanova, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Potential+roles+of+melatonin+and+chronotherapy+among+the+new+trends+in+hypertension+treatment.">Potential roles of melatonin and chronotherapy among the new trends in hypertension treatment.</a> J Pineal Res. 47 (2), 127-133 (2009).</li><li>Li, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=H-type+hypertension+is+a+risk+factor+for+cerebral+small-vessel+disease.">H-type hypertension is a risk factor for cerebral small-vessel disease.</a> BioMed Res Int. 2020, 6498903 (2020).</li><li>Rodrigo, R., Passalacqua, W., Araya, J., Orellana, M., Rivera, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homocysteine+and+essential+hypertension.">Homocysteine and essential hypertension.</a> J Clin Pharmacol. 43 (12), 1299-1306 (2003).</li><li>Lehmann, M., Gottfries, C. G., Regland, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+cognitive+impairment+in+the+elderly:+Homocysteine+is+an+early+marker.">Identification of cognitive impairment in the elderly: Homocysteine is an early marker.</a> Dement Geriatr Cogn. 10 (1), 12-20 (1999).</li><li>dos Santos, E. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evidence+that+folic+acid+deficiency+is+a+major+determinant+of+hyperhomocysteinemia+in+Parkinson's+disease.">Evidence that folic acid deficiency is a major determinant of hyperhomocysteinemia in Parkinson's disease.</a> Metab Brain Dis. 24 (2), 257-269 (2009).</li><li>Zhao, W., Gao, F., Lv, L., Chen, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+interaction+of+hypertension+and+homocysteine+increases+the+risk+of+mortality+among+middle-aged+and+older+population+in+the+United+States.">The interaction of hypertension and homocysteine increases the risk of mortality among middle-aged and older population in the United States.</a> J Hypertens. 40 (2), 254-263 (2022).</li><li>Woo, K. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hyperhomocyst(e)inemia+is+a+risk+factor+for+arterial+endothelial+dysfunction+in+humans.">Hyperhomocyst(e)inemia is a risk factor for arterial endothelial dysfunction in humans.</a> Circulation. 96 (8), 2542-2544 (1997).</li><li>Lu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+intervention+of+enalapril+maleate+and+folic+acid+tablet+on+the+expressions+of+the+grp78+and+chop+and+vascular+remodeling+in+the+vascular+smooth+muscle+cells+of+h-hypertensive+rats+with+homocysteine.">The intervention of enalapril maleate and folic acid tablet on the expressions of the grp78 and chop and vascular remodeling in the vascular smooth muscle cells of h-hypertensive rats with homocysteine.</a> Eur Rev Med Pharmaco. 22 (7), 2160-2168 (2018).</li><li>L&#243;pez-Garc&#237;a, P., Moreira, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+forces+for+the+origin+of+the+eukaryotic+nucleus.">Selective forces for the origin of the eukaryotic nucleus.</a> BioEssays. 28 (5), 525-533 (2006).</li><li>Minamino, T., Komuro, I., Kitakaze, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endoplasmic+reticulum+stress+as+a+therapeutic+target+in+cardiovascular+disease.">Endoplasmic reticulum stress as a therapeutic target in cardiovascular disease.</a> Circ Res. 107 (9), 1071-1082 (2010).</li><li>Chen, X., Cubillos-Ruiz, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endoplasmic+reticulum+stress+signals+in+the+tumour+and+its+microenvironment.">Endoplasmic reticulum stress signals in the tumour and its microenvironment.</a> Nat Rev Cancer. 21 (2), 71-88 (2021).</li><li>Ji, C., Kaplowitz, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hyperhomocysteinemia,+endoplasmic+reticulum+stress,+and+alcoholic+liver+injury.">Hyperhomocysteinemia, endoplasmic reticulum stress, and alcoholic liver injury.</a> World J Gastroentero. 10 (12), 1699-1708 (2004).</li><li>Zhang, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homocysteine+induces+programmed+cell+death+in+human+vascular+endothelial+cells+through+activation+of+the+unfolded+protein+response.">Homocysteine induces programmed cell death in human vascular endothelial cells through activation of the unfolded protein response.</a> J Biol Chem. 276 (38), 35867-35874 (2001).</li><li>Cunard, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endoplasmic+reticulum+stress,+a+driver+or+an+innocent+bystander+in+endothelial+dysfunction+associated+with+hypertension.">Endoplasmic reticulum stress, a driver or an innocent bystander in endothelial dysfunction associated with hypertension.</a> Cur Hypertens Rep. 19 (8), 64 (2017).</li><li>Casas, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=GRP78+at+the+centre+of+the+stage+in+cancer+and+neuroprotection.">GRP78 at the centre of the stage in cancer and neuroprotection.</a> Front Neurosci. 11, 177 (2017).</li><li>Urano, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coupling+of+stress+in+the+ER+to+activation+of+JNK+protein+kinases+by+transmembrane+protein+kinase+ire1.">Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase ire1.</a> Science. 287 (5453), 664-666 (2000).</li><li>Borghi, A., Verstrepen, L., Beyaert, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TRAF2+multitasking+in+tnf+receptor-induced+signaling+to+nf-&#954;b,+map+kinases+and+cell+death.">TRAF2 multitasking in tnf receptor-induced signaling to nf-&#954;b, map kinases and cell death.</a> Biochem Pharmacol. 116, 1-10 (2016).</li><li>Wen, X. -. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Butylphthalide+suppresses+neuronal+cells+apoptosis+and+inhibits+JNK-caspase3+signaling+pathway+after+brain+ischemia+/reperfusion+in+rats.">Butylphthalide suppresses neuronal cells apoptosis and inhibits JNK-caspase3 signaling pathway after brain ischemia /reperfusion in rats.</a> Cell Mol Neurobiol. 36 (7), 1087-1095 (2016).</li><li>Jin, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serine-threonine+protein+kinase+activation+may+be+an+effective+target+for+reducing+neuronal+apoptosis+after+spinal+cord+injury.">Serine-threonine protein kinase activation may be an effective target for reducing neuronal apoptosis after spinal cord injury.</a> Neural Regen Res. 10 (11), 1830-1835 (2015).</li><li>Xu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrogen+and+propofol+combination+therapy+inhibits+endoplasmic+reticulum+stress+and+remarkably+attenuates+cerebral+ischemia-reperfusion+injury+and+ogd+injury+in+hippocampus.">Estrogen and propofol combination therapy inhibits endoplasmic reticulum stress and remarkably attenuates cerebral ischemia-reperfusion injury and ogd injury in hippocampus.</a> Biomed Pharmacother. 108, 1596-1606 (2018).</li></ol>

우리는 연구가 잠재적인 이해 상충으로 해석될 수 있는 상업적 또는 재정적 관계가 없는 상태에서 수행되었음을 선언합니다.

고혈압은 성인 인구의 1/3에 영향을 미치는 가장 흔한 심혈관 질환 중 하나이며 뇌졸중, 관상 동맥 심장 질환, 심부 및 신부전의 위험을 증가시킵니다8. H형 고혈압은 원발성 고혈압과 호모시스테인 수치 증가가 동시에 발생하는 특수한 유형의 고혈압으로, 수년 동안 많은 관심을 받아왔다9. 최근에는 항고혈압제와 병용한 경구용 EM의 사용으로 혈압 조절 및 혈중...

심장마비, 뇌졸중, 신부전의 주요 위험 요인인 고혈압은 전 세계 10억 명의 사람들에게 영향을 미치는 중요한 공중 보건 문제가 되었습니다1. 호모시스테인(Hcy)은 티올기 함유 아미노산으로 메티오닌 대사의 중요한 대사 중개자입니다. 혈장 Hcy 수치가 상승된 고혈압은 H형 고혈압으로 정의되며, 이는 뇌졸중 2,3과 같은 심뇌혈관 질환의 발생 및 재발에 대한 중요한 위험 요인이 될 수 있습니다. 최근 연구에 따르면 H형 고혈압의 동반 거주는 심혈관 및 뇌혈관 질환의 부작용을 악화시킬 수 있다고 보고되었다4. 특히 중국에서 H형 고혈압 환자의 75%가 원발성 고혈압을 앓고 있으며, 이는 삶의 질에 심각한 영향을 미친다5. 현재 H형 고혈압의 치료는 주로 서양 의학을 포함하고 있습니다. 그러나 특정 부작용과 순응도 저하를 유발할 수 있으며 더 이상 H형 고혈압의 종합적인 관리에 대한 요구를 충족시킬 수 없습니다.
중국 전통 의학(TCM)은 중국에서 2,000년 이상의 역사를 가진 독특한 자원입니다. 서양 의학에서 고혈압 조절에 대한 충족되지 않은 요구로 인해 임상의들은 H형 고혈압의 예방 및 치료에서 한의학의 잠재적 역할을 고려하기 시작했다6. Huotan Jiedu Tongluo Decoction (HTJDTLD)은 Yue Deng 교수가 광범위한 임상 전문 지식을 바탕으로 공식화한 중국 전통 의학 공식입니다7. 20년 이상의 임상 적용 과정에서 HTJDTLD는 심혈관 및 뇌혈관 질환 치료에서 놀라운 효과를 입증했습니다1. 그러나 HTJDTLD가 H형 고혈압에 치료 효과가 있는지는 보고되지 않았습니다. 따라서 H형 고혈압 환자에서 HTJDTLD의 항고혈압 효과와 특정 기전을 탐색하고 H형 고혈압 치료를 위한 잠재적 치료 약물을 식별하는 것을 목표로 했습니다.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1st Strand cDNA Synthesis SuperMix for qPCR</td>
			<td>Yeasen,China</td>
			<td>11149ES</td>
			<td>cDNA synthesis kit&#160;</td>
		</tr>
		<tr>
			<td>Anti-beta-actin antibody</td>
			<td>Bioss, China</td>
			<td>bs-0061R</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-caspase-3 antibody</td>
			<td>Bioss, China</td>
			<td>bs-0081R</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-GPR78 antibody</td>
			<td>Abcam, USA</td>
			<td>ab108513</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-JNK antibody</td>
			<td>Abcam, USA</td>
			<td>ab76572</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-p-JNK antibody</td>
			<td>Bioss, China</td>
			<td>bsm-52462R</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-rabbit IgG antibody</td>
			<td>Bioss, China</td>
			<td>bs-0295G-HRP</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-TRAF2 antibody</td>
			<td>Bioss, China</td>
			<td>bs-22372R</td>
			<td></td>
		</tr>
		<tr>
			<td>Bio-Rad CFX96 Touch system&#160;</td>
			<td>Bio-Rad</td>
			<td>CFX96</td>
			<td>real-time PCR detection system&#160;</td>
		</tr>
		<tr>
			<td>ECL Western Blot Substrates</td>
			<td>Merck, MA, USA</td>
			<td>WBULP-10ML</td>
			<td></td>
		</tr>
		<tr>
			<td>Enalapril maleate folic acid tablets</td>
			<td>Yangzijiang Pharmaceutical Company, China</td>
			<td>20040991</td>
			<td></td>
		</tr>
		<tr>
			<td>FastStart SYBR Green Master</td>
			<td>Sigma</td>
			<td>FSSGMMRO</td>
			<td></td>
		</tr>
		<tr>
			<td>Fructus Trichosanthis</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Hirudo</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>intelligent noninvasive sphygmomanometer&#160;</td>
			<td>Beijing Softron Biotechnology company</td>
			<td>BP-2010A</td>
			<td></td>
		</tr>
		<tr>
			<td>Lonicerae Japonicae Flos</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Methionine</td>
			<td>Sigma, USA</td>
			<td>M9500</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix Angelicae Sinensis</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Glycyrrhizae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Nardostachyos</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Rhodiolae Crenulatae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix et Rhizoma Salviae Miltiorrhizae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Radix Scrophulariae</td>
			<td>The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, China</td>
			<td>No catalog number</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat Hcy ELISA Kits</td>
			<td>Shanghai Meimian Industrial Company, China</td>
			<td>MM-0293R2</td>
			<td></td>
		</tr>
		<tr>
			<td>RIPA buffer</td>
			<td>Shanghai Beyotime Biotechnology company</td>
			<td>P0013B</td>
			<td></td>
		</tr>
	</tbody>
</table>

the antihypertensive effects and mechanisms of huotan jiedu tongluo decoction in rats with h-type hypertension

혈장 호모시스테인(Hcy) 수치 상승을 특징으로 하는 특정 형태의 고혈압인 H형 고혈압은 전 세계적으로 주요 공중 보건 문제가 되었습니다. 이 연구는 혈관 협착증 치료에 일반적으로 사용되는 매우 효과적인 중국 전통 의학 공식인 Huotan Jiedu Tongluo 달인(HTJDTLD)의 저혈압 효과와 기저 기전을 조사했습니다. 쥐에서 H형 고혈압을 유발하기 위해 메티오닌을 사용하였고, HTJDTLD를 위내로 투여하였다. 그런 다음, 쥐의 꼬리 동맥의 수축기 및 이완기 혈압을 비침습적 쥐 꼬리 압력계로 측정했습니다. 대동맥의 조직학적 평가는 hematoxylin-eosin(HE) 염색에 의해 수행되었습니다. 효소 결합 면역 흡착 분석법(ELISA)을 사용하여 Hcy 수준을 측정하고, 정량적 역전사 효소 중합효소 연쇄 반응(qRT-PCR) 및 웨스턴 블로팅을 사용하여 포도당 조절 단백질 78(GRP78), 종양 괴사 인자(TNF) 수용체 관련 인자 2(TRAF2), c-Jun N-말단 키나아제(JNK) 및 카스파제-3의 mRNA 및 단백질 수준을 측정했습니다. 그 결과, HTJDTLD는 메티오닌 치료 후 혈압을 유의하게 낮추고, 조직병리학적 병변을 완화하며, Hcy 수치를 감소시키는 것으로 나타났습니다. 또한, HTJDTLD는 주로 소포체(ER) 스트레스 유발 세포사멸 경로에 관여하는 GRP78, JNK, TRAF2 및 caspase 3의 유전자 및 단백질 발현을 유의하게 억제했습니다. 전반적으로, 결과는 HTJDTLD가 H형 고혈압이 있는 쥐에서 효과적인 항고혈압 효과를 나타냈으며, ER 스트레스 유발 세포자멸사 경로 활성화 억제와 관련된 항고혈압 기전을 밝혔다.

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

저자 스포트라이트: 항고혈압제로서의 Huotan Jiedu Tongluo 달인 탐색

H형 고혈압을 가진 쥐에서 Huotan Jiedu Tongluo 달인의 항고혈압 효과 및 메커니즘

표 4 및 표 5에서 보는 바와 같이, 수축기 혈압(SBP)과 이완기 혈압(DBP)은 1주에서 4주 사이에 CON 그룹보다 MET 그룹에서 유의하게 높았다. HTJDTLD 처리 후, 쥐의 SBP와 DBP는 MET 그룹보다 유의하게 낮았다. 특히, HTJDTLD와 EM을 병용하면 HTJDTLD 단독 치료보다 강력한 항고혈압 효과가 나타났다.
HE 염색 및 Masson의 삼색 염색에 따르면, 대동맥 혈관 벽의 자궁 내막?...

Read this Scientific Article Protocol about The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension at JoVE.com

여기에서는 H형 고혈압을 유도하기 위한 프로토콜을 제시하고 위내 투여 Huotan Jiedu Tongluo 달인(HTJDTLD)의 항고혈압 효과를 평가합니다. H형 고혈압이 있는 쥐에서 HTJDTLD는 효과적인 항고혈압 효과를 보였는데, 이는 소포체(ER) 스트레스 유발 세포자멸사 경로 활성화 억제와 관련이 있을 수 있습니다.

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

H형 고혈압은 인간의 중요한 공중 보건 과제가 되었습니다. 주로 서양 의학 치료에 의존하는데, 이는 슬픈 결과를 초래할 수 있습니다. 본 연구는 H형 고혈압이 있는 쥐를 대상으로 한의학 제제인 Huotan Jiedu Tongluo 달인의 항고혈압 효과와 기전을 조사한다.우리의 연구는 Huotan Jiedu Tongluo 달인이 H 형 고혈압을 가진 쥐에서 항 고혈압 효과가 있음을 나타 냈습니다. 근본적인 메커니즘은 환자에게 효과적인 치료 옵션을 제공하는 ER 스트레스 활성화 유발 세포 사멸을 억제하는 것을 포함할 수 있습니다. 향후 H형 고혈압에 대한 조기진단과 예후를 위한 잠재적 바이오마커를 폭발적으로 발굴하고 새로운 치료약을 개발해 나갈 것입니다.

the-antihypertensive-effects-mechanisms-huotan-jiedu-tongluo

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JoVE Journal

Biochemistry

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

432 조회수.  Changchun University of Chinese Medicine. H형 고혈압은 인간의 중요한 공중 보건 과제가 되었습니다. 주로 서양 의학 치료에 의존하는데, 이는 슬픈 결과를 초래할 수 있습니다. 본 연구는 H형 고혈압이 있는 쥐를 대상으로 한의학 제제인 Huotan Jiedu Tongluo 달인의 항고혈압 효과와 기전을 조사한다.우리의 연구는 Huotan Jiedu Tongluo 달인이 H 형 고혈압을 가진 쥐에서 항 고혈압 효과가 있음을 나타 냈습니다. 근본적인 ...

비디오: 저자 스포트라이트: 항고혈압제로서의 Huotan Jiedu Tongluo 달인 탐색

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health challenge worldwide. This study investigated the hypotensive effects and underlying mechanisms of Huotan Jiedu Tongluo decoction (HTJDTLD), a highly effective traditional Chinese medicine formula commonly used to treat vascular stenosis. Methionine was used to induce H-type hypertension in rats, and HTJDTLD was administered intragastrically. Then, the systolic and diastolic blood pressures of the caudal artery of rats were measured by noninvasive rat caudal manometry. Histological assessment of the aorta was performed by hematoxylin-eosin (HE) staining. Enzyme-linked immunosorbent assay (ELISA) was used to measure Hcy levels, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting were used to determine the mRNA and protein levels of Glucose regulatory protein 78 (GRP78), Tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2), c-Jun N-terminal&#160;kinases (JNK), and caspase-3. The results showed that HTJDTLD significantly lowered blood pressure, alleviated histopathological lesions, and decreased Hcy levels after methionine treatment. Moreover, HTJDTLD significantly inhibited the gene and protein expression of GRP78, JNK, TRAF2, and caspase 3, which are involved mainly in the endoplasmic reticulum (ER) stress-induced apoptosis pathway. Overall, the results indicated that HTJDTLD had effective antihypertensive effects in rats with H-type hypertension and revealed the antihypertensive mechanisms associated with inhibition of ER stress-induced apoptosis pathway activation.

Here, we present a protocol to induce H-type hypertension and evaluate the antihypertensive effects of Huotan Jiedu Tongluo decoction (HTJDTLD) administered intragastrically. In rats with H-type hypertension, HTJDTLD had effective antihypertensive effects, possibly associated with inhibition of endoplasmic reticulum (ER) stress-induced apoptosis pathway activation.

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Methods Article

저자 스포트라이트: 항고혈압제로서의 Huotan Jiedu Tongluo 달인 탐색