Biological Physics Research Group,
Clarendon Laboratory,
Department of Physics,
Biological Physics Research Group, Clarendon Laboratory, Department of Physics,
Clarendon Laboratory, Department of Physics
Achillefs N. Kapanidis has not added Biography.
If you are Achillefs N. Kapanidis and would like to personalize this page please email our Author Liaison for assistance.
Three-color alternating-laser excitation of single molecules: monitoring multiple interactions and distances.
Biophysical journal Jan, 2007 | Pubmed ID: 17040983
Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex.
Cell Mar, 2002 | Pubmed ID: 11893332
The power and prospects of fluorescence microscopies and spectroscopies.
Annual review of biophysics and biomolecular structure , 2003 | Pubmed ID: 12598370
Fluorescence resonance energy transfer (FRET) in analysis of transcription-complex structure and function.
Methods in enzymology , 2003 | Pubmed ID: 14712697
Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules.
Proceedings of the National Academy of Sciences of the United States of America Jun, 2004 | Pubmed ID: 15175430
Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation.
Biophysical journal Apr, 2005 | Pubmed ID: 15653725
Alternating-laser excitation of single molecules.
Accounts of chemical research Jul, 2005 | Pubmed ID: 16028886
Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis.
Molecular cell Nov, 2005 | Pubmed ID: 16285917
Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes.
Biophysical journal Feb, 2006 | Pubmed ID: 16299085
Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism.
Science (New York, N.Y.) Nov, 2006 | Pubmed ID: 17110578
Periodic acceptor excitation spectroscopy of single molecules.
European biophysics journal : EBJ Jul, 2007 | Pubmed ID: 17279362
Red light, green light: probing single molecules using alternating-laser excitation.
Biochemical Society transactions Aug, 2008 | Pubmed ID: 18631150
Reconfigurable, braced, three-dimensional DNA nanostructures.
Nature nanotechnology Feb, 2008 | Pubmed ID: 18654468
Biology, one molecule at a time.
Trends in biochemical sciences May, 2009 | Pubmed ID: 19362843
Single-molecule FRET analysis of protein-DNA complexes.
Methods in molecular biology (Clifton, N.J.) , 2009 | Pubmed ID: 19378183
A facile method for reversibly linking a recombinant protein to DNA.
Chembiochem : a European journal of chemical biology Jun, 2009 | Pubmed ID: 19449345
DNA monofunctionalization of quantum dots.
Chembiochem : a European journal of chemical biology Jul, 2009 | Pubmed ID: 19554595
Probing biomolecular structures and dynamics of single molecules using in-gel alternating-laser excitation.
Analytical chemistry Dec, 2009 | Pubmed ID: 19863108
Single-molecule DNA biosensors for protein and ligand detection.
Angewandte Chemie (International ed. in English) Feb, 2010 | Pubmed ID: 20077444
Conformational transitions in DNA polymerase I revealed by single-molecule FRET.
Proceedings of the National Academy of Sciences of the United States of America Jan, 2010 | Pubmed ID: 20080740
Characterizing single-molecule FRET dynamics with probability distribution analysis.
Chemphyschem : a European journal of chemical physics and physical chemistry Jul, 2010 | Pubmed ID: 20575136
Surfing on a new wave of single-molecule fluorescence methods.
Physical biology , 2010 | Pubmed ID: 20686191
Monitoring multiple distances within a single molecule using switchable FRET.
Nature methods Oct, 2010 | Pubmed ID: 20818380
Sensing DNA opening in transcription using quenchable Förster resonance energy transfer.
Biochemistry Nov, 2010 | Pubmed ID: 20818825
Defining the limits of single-molecule FRET resolution in TIRF microscopy.
Biophysical journal Nov, 2010 | Pubmed ID: 21044609
Improved temporal resolution and linked hidden Markov modeling for switchable single-molecule FRET.
Chemphyschem : a European journal of chemical physics and physical chemistry Feb, 2011 | Pubmed ID: 21280168
Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis.
Biophysical journal Mar, 2011 | Pubmed ID: 21402040
DAOSTORM: an algorithm for high- density super-resolution microscopy.
Nature methods Apr, 2011 | Pubmed ID: 21451515
Colorful molecular diagnostics.
Clinical chemistry Apr, 2012 | Pubmed ID: 22300628
A protein biosensor that relies on bending of single DNA molecules.
Chemphyschem : a European journal of chemical physics and physical chemistry Mar, 2012 | Pubmed ID: 22368093
Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET.
Biophysical journal Jun, 2012 | Pubmed ID: 22713582
T7 RNA polymerase functions in vitro without clustering.
PloS one , 2012 | Pubmed ID: 22768341
Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence.
Proceedings of the National Academy of Sciences of the United States of America Dec, 2012 | Pubmed ID: 23184986
Six steps closer to FRET-driven structural biology.
Nature methods Dec, 2012 | Pubmed ID: 23223168
The transcription bubble of the RNA polymerase-promoter open complex exhibits conformational heterogeneity and millisecond-scale dynamics: implications for transcription start-site selection.
Journal of molecular biology Mar, 2013 | Pubmed ID: 23274143
Non-covalent single transcription factor encapsulation inside a DNA cage.
Angewandte Chemie (International ed. in English) Feb, 2013 | Pubmed ID: 23325751
Single-molecule DNA repair in live bacteria.
Proceedings of the National Academy of Sciences of the United States of America May, 2013 | Pubmed ID: 23630273
Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid.
Proceedings of the National Academy of Sciences of the United States of America Jul, 2013 | Pubmed ID: 23818620
Multiscale spatial organization of RNA polymerase in Escherichia coli.
Biophysical journal Jul, 2013 | Pubmed ID: 23823236
Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion.
Nature communications , 2013 | Pubmed ID: 23831915
Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes.
Proceedings of the National Academy of Sciences of the United States of America Oct, 2013 | Pubmed ID: 24101525
Long-lived intracellular single-molecule fluorescence using electroporated molecules.
Biophysical journal Dec, 2013 | Pubmed ID: 24314075
Studying the organization of DNA repair by single-cell and single-molecule imaging.
DNA repair Aug, 2014 | Pubmed ID: 24629485
In vivo single-molecule imaging of bacterial DNA replication, transcription, and repair.
FEBS letters Oct, 2014 | Pubmed ID: 24859634
Single-molecule FRET reveals a corkscrew RNA structure for the polymerase-bound influenza virus promoter.
Proceedings of the National Academy of Sciences of the United States of America Aug, 2014 | Pubmed ID: 25071209
Tethered fluorophore motion: studying large DNA conformational changes by single-fluorophore imaging.
Biophysical journal Sep, 2014 | Pubmed ID: 25185556
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved