Achillefs N. Kapanidis

Three-color alternating-laser excitation of single molecules: monitoring multiple interactions and distances.

Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex.

The power and prospects of fluorescence microscopies and spectroscopies.

Fluorescence resonance energy transfer (FRET) in analysis of transcription-complex structure and function.

Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules.

Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation.

Alternating-laser excitation of single molecules.

Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis.

Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes.

Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism.

Periodic acceptor excitation spectroscopy of single molecules.

Red light, green light: probing single molecules using alternating-laser excitation.

Reconfigurable, braced, three-dimensional DNA nanostructures.

Biology, one molecule at a time.

Single-molecule FRET analysis of protein-DNA complexes.

A facile method for reversibly linking a recombinant protein to DNA.

DNA monofunctionalization of quantum dots.

Probing biomolecular structures and dynamics of single molecules using in-gel alternating-laser excitation.

Single-molecule DNA biosensors for protein and ligand detection.

Conformational transitions in DNA polymerase I revealed by single-molecule FRET.

Characterizing single-molecule FRET dynamics with probability distribution analysis.

Surfing on a new wave of single-molecule fluorescence methods.

Monitoring multiple distances within a single molecule using switchable FRET.

Sensing DNA opening in transcription using quenchable Förster resonance energy transfer.

Defining the limits of single-molecule FRET resolution in TIRF microscopy.

Improved temporal resolution and linked hidden Markov modeling for switchable single-molecule FRET.

Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis.

DAOSTORM: an algorithm for high- density super-resolution microscopy.

Colorful molecular diagnostics.

A protein biosensor that relies on bending of single DNA molecules.

Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET.

T7 RNA polymerase functions in vitro without clustering.

Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence.

Six steps closer to FRET-driven structural biology.

The transcription bubble of the RNA polymerase-promoter open complex exhibits conformational heterogeneity and millisecond-scale dynamics: implications for transcription start-site selection.

Non-covalent single transcription factor encapsulation inside a DNA cage.

Single-molecule DNA repair in live bacteria.

Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid.

Multiscale spatial organization of RNA polymerase in Escherichia coli.

Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion.

Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes.

Long-lived intracellular single-molecule fluorescence using electroporated molecules.

Studying the organization of DNA repair by single-cell and single-molecule imaging.

In vivo single-molecule imaging of bacterial DNA replication, transcription, and repair.

Single-molecule FRET reveals a corkscrew RNA structure for the polymerase-bound influenza virus promoter.

Tethered fluorophore motion: studying large DNA conformational changes by single-fluorophore imaging.