Henning Urlaub

A two-tracked approach to analyze RNA-protein crosslinking sites in native, nonlabeled small nuclear ribonucleoprotein particles.

Single-stranded antisense siRNAs guide target RNA cleavage in RNAi.

Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.

Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins.

Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome.

Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method.

An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains.

Mutagenesis suggests several roles of Snu114p in pre-mRNA splicing.

Analysis of mammalian gene function using small interfering RNAs.

The zinc finger-associated domain of the Drosophila transcription factor grauzone is a novel zinc-coordinating protein-protein interaction module.

Analysis of site-specific protein-RNA cross-links in isolated RNP complexes, combining affinity selection and mass spectrometry.

The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.

A subset of human 35S U5 proteins, including Prp19, function prior to catalytic step 1 of splicing.

Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding.

Purification, crystallization and preliminary crystallographic data of the m3G cap-binding domain of human snRNP import factor snurportin 1.

Assembly and maturation of the U3 snoRNP in the nucleoplasm in a large dynamic multiprotein complex.

Characterization of the cleavage site and function of resulting cleavage fragments after limited proteolysis of Clostridium difficile toxin B (TcdB) by host cells.

Proteomic analysis of reaper 5' untranslated region-interacting factors isolated by tobramycin affinity-selection reveals a role for La antigen in reaper mRNA translation.

Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila.

Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif.

Identification of novel argonaute-associated proteins.

Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles.

DDX6 recruits translational silenced human reticulocyte 15-lipoxygenase mRNA to RNP granules.

RNA structure and RNA-protein interactions in purified yeast U6 snRNPs.

Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.

Identification, cloning, and functional analysis of the human U6 snRNA-specific terminal uridylyl transferase.

Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions.

The Methanothermobacter thermautotrophicus ExoIII homologue Mth212 is a DNA uridine endonuclease.

Protein composition of human mRNPs spliced in vitro and differential requirements for mRNP protein recruitment.

Molecular anatomy of a trafficking organelle.

Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus.

Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.

Detection of protein-RNA crosslinks by NanoLC-ESI-MS/MS using precursor ion scanning and multiple reaction monitoring (MRM) experiments.

In vitro RNA editing in plant mitochondria does not require added energy.

Role of cdk2 in the sequential phosphorylation/activation of C/EBPbeta during adipocyte differentiation.

Improved identification of enriched peptide RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry.

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

Two DEAD-box proteins may be part of RNA-dependent high-molecular-mass protein complexes in Arabidopsis mitochondria.

GraFix: sample preparation for single-particle electron cryomicroscopy.

'Proteomic basics--sample preparation and separation': the 1st European Summer School in Kloster Neustift, 12-18 August, 2007 Brixen/Bressanone, South Tyrol, Italy.

Spatial interplay between PIASy and FIP200 in the regulation of signal transduction and transcriptional activity.

Isolation of an active step I spliceosome and composition of its RNP core.

CASK Functions as a Mg2+-independent neurexin kinase.

Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome.

Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25.

Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes.

Analyzing RNA-protein crosslinking sites in unlabeled ribonucleoprotein complexes by mass spectrometry.

Stalling of spliceosome assembly at distinct stages by small-molecule inhibitors of protein acetylation and deacetylation.

Structure of tripeptidyl-peptidase I provides insight into the molecular basis of late infantile neuronal ceroid lipofuscinosis.

SAP18 promotes Krüppel-dependent transcriptional repression by enhancer-specific histone deacetylation.

Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.

Atmospheric pressure free liquid infrared MALDI mass spectrometry: toward a combined ESI/ MALDI-liquid chromatography interface.

The neuropeptidome of Rhodnius prolixus brain.

Enrichment of protein-RNA crosslinks from crude UV-irradiated mixtures for MS analysis by on-line chromatography using titanium dioxide columns.

Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs.

SLP-65 phosphorylation dynamics reveals a functional basis for signal integration by receptor-proximal adaptor proteins.

iTRAQ-labeling of in-gel digested proteins for relative quantification.

SMN-assisted assembly of snRNP-specific Sm cores in trypanosomes.

The cytoplasmic peptidase DPP9 is rate-limiting for degradation of proline-containing peptides.

Initial insight into the function of the lysosomal 66.3 kDa protein from mouse by means of X-ray crystallography.

"ChopNSpice," a mass spectrometric approach that allows identification of endogenous small ubiquitin-like modifier-conjugated peptides.

In vivo expression and purification of aptamer-tagged small RNA regulators.

Multimerization and H3K9me3 binding are required for CDYL1b heterochromatin association.

A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.

The B-lymphoid Grb2 interaction code.

Proteomic basics - Quantification and post-translational modifications of proteins: The 3rd European summer school in Kloster Neustift.

Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components.

The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome.

Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.

Essential role of a trypanosome U4-specific Sm core protein in small nuclear ribonucleoprotein assembly and splicing.

Crystal structure of an intramolecular chaperone mediating triple-beta-helix folding.

Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation.

Molecular architecture of the human Prp19/CDC5L complex.

A Syd-1 homologue regulates pre- and postsynaptic maturation in Drosophila.

Determination of protein stoichiometry within protein complexes using absolute quantification and multiple reaction monitoring.

Extraction of proteins from formalin-fixed, paraffin-embedded tissue using the Qproteome extraction technique and preparation of tryptic peptides for liquid chromatography/mass spectrometry analysis.

Exon definition complexes contain the tri-snRNP and can be directly converted into B-like precatalytic splicing complexes.

Mapping the binding site of snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry.

Merging molecular electron microscopy and mass spectrometry by carbon film-assisted endoproteinase digestion.

The archaeal Lsm protein binds to small RNAs.

The human Pat1b protein: a novel mRNA deadenylation factor identified by a new immunoprecipitation technique.

Pseudo-neutral-loss scan for selective detection of phosphopeptides and N-glycopeptides using liquid chromatography coupled with a hybrid linear ion-trap/orbitrap mass spectrometer.

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.

A novel Nop5-sRNA interaction that is required for efficient archaeal box C/D sRNP formation.

Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis.

PERILIPIN-dependent control of lipid droplet structure and fat storage in Drosophila.

Phosphorylation of human Argonaute proteins affects small RNA binding.

7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.

Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the SMN (survival of motoneuron) pre-mRNA.

Fc gamma receptor IIb modulates the molecular Grb2 interaction network in activated B cells.

RACK1 is a novel interaction partner of PTK7 that is required for neural tube closure.

Complex phosphorylation dynamics control the composition of the Syk interactome in B cells.

A cytoplasmic complex mediates specific mRNA recognition and localization in yeast.

Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method.

Functional proteomics of neuropeptidome dynamics during the feeding process of Rhodnius prolixus.

B cell receptor-mediated antigen gathering requires ubiquitin ligase Cbl and adaptors Grb2 and Dok-3 to recruit dynein to the signaling microcluster.

Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells.

Sumoylation inhibits alpha-synuclein aggregation and toxicity.

Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation.

The B-cell antigen receptor signals through a preformed transducer module of SLP65 and CIN85.

Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patterns.

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching.

Optic atrophy 1 is an A-kinase anchoring protein on lipid droplets that mediates adrenergic control of lipolysis.

Phosphorylation site localization in peptides by MALDI MS/MS and the Mascot Delta Score.

MINOS1 is a conserved component of mitofilin complexes and required for mitochondrial function and cristae organization.

BC1-FMRP interaction is modulated by 2'-O-methylation: RNA-binding activity of the tudor domain and translational regulation at synapses.

Syk kinase-coupled C-type lectin receptors engage protein kinase C-σ to elicit Card9 adaptor-mediated innate immunity.

Late motor decline after accomplished remyelination: impact for progressive multiple sclerosis.

Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.

OKB, a novel family of brain-gut neuropeptides from insects.

The cryo-EM structure of the UPF-EJC complex shows UPF1 poised toward the RNA 3' end.

The guanine nucleotide exchange factor Vav2 is a negative regulator of parathyroid hormone receptor/Gq signaling.

Investigation of protein-RNA interactions by mass spectrometry--Techniques and applications.

Quantitative mass spectrometry-based proteomics: an overview.

Absolute quantification of proteins using standard peptides and multiple reaction monitoring.

Identification of endogenous SUMO1 accepter sites by mass spectrometry.

Determinants of interaction specificity of the Bacillus subtilis GlcT antitermination protein: functionality and phosphorylation specificity depend on the arrangement of the regulatory domains.

Structure and activity of the only human RNase T2.

Biophysical properties and cellular toxicity of covalent crosslinked oligomers of α-synuclein formed by photoinduced side-chain tyrosyl radicals.

Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion.

Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions.

The claudin Megatrachea protein complex.

The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA.

The human cytomegalovirus DNA polymerase processivity factor UL44 is modified by SUMO in a DNA-dependent manner.

Systematic analysis of barrier-forming FG hydrogels from Xenopus nuclear pore complexes.

Burial of the polymorphic residue 129 in amyloid fibrils of prion stop mutants.

In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice.

The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters.

EF-P is essential for rapid synthesis of proteins containing consecutive proline residues.

Identification of CRM1-dependent Nuclear Export Cargos Using Quantitative Mass Spectrometry.

Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex.

Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia.

SCFFbxw5 mediates transient degradation of actin remodeller Eps8 to allow proper mitotic progression.

Evolution of the protein stoichiometry in the L12 stalk of bacterial and organellar ribosomes.

Mass spectrometry imaging: linking molecule profiles to tissue spatial distribution.

Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system.

Mitochondrial SIRT4-type proteins in Caenorhabditis elegans and mammals interact with pyruvate carboxylase and other acetylated biotin-dependent carboxylases.

An automated in-gel digestion/iTRAQ-labeling workflow for robust quantification of gel-separated proteins.

Detecting endogenous SUMO targets in mammalian cells and tissues.

β2 integrin-derived signals induce cell survival and proliferation of AML blasts by activating a Syk/STAT signaling axis.

Anle138b: a novel oligomer modulator for disease-modifying therapy of neurodegenerative diseases such as prion and Parkinson's disease.

Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.

Mover is a homomeric phospho-protein present on synaptic vesicles.

The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis.

Choroid plexus transcytosis and exosome shuttling deliver folate into brain parenchyma.

Structural analysis of the yeast Dhh1-Pat1 complex reveals how Dhh1 engages Pat1, Edc3 and RNA in mutually exclusive interactions.

Systematic identification of proteins binding to chromatin-embedded ubiquitylated H2B reveals recruitment of SWI/SNF to regulate transcription.

Increased proteome coverage by combining PAGE and peptide isoelectric focusing: comparative study of gel-based separation approaches.

The heme a synthase Cox15 associates with cytochrome c oxidase assembly intermediates during Cox1 maturation.

Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.

The SUMO1-E67 interacting loop peptide is an allosteric inhibitor of the dipeptidyl peptidases 8 and 9.

Immunostimulatory activity of murine keratinocyte-derived exosomes.

Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.

Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry.

Cutting edge: feed-forward activation of phospholipase Cγ2 via C2 domain-mediated binding to SLP65.

Phosphorylation drives a dynamic switch in serine/arginine-rich proteins.

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.

Mass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count.

A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii.

In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex.

The NHL domain of BRAT is an RNA-binding domain that directly contacts the hunchback mRNA for regulation.

Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D.

Separation methodology to improve proteome coverage depth.

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.

Biophysical and biochemical analysis of hnRNP K: arginine methylation, reversible aggregation and combinatorial binding to nucleic acids.

Analyzing the protein assembly and dynamics of the human spliceosome with SILAC.

Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family.

Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins.

Modulation of TCR responsiveness by the Grb2-family adaptor, Gads.

RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus.

Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family.

Dual tagging as an approach to isolate endogenous chromatin remodeling complexes from Saccharomyces cerevisiae.

Effects of mycophenolate mofetil on kidney function and phosphorylation status of renal proteins in Alport COL4A3-deficient mice.

Studying macromolecular complex stoichiometries by peptide-based mass spectrometry.

The RNA helicase Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes.

FLT3-ITD and TLR9 employ Bruton's tyrosine kinase to activate distinct transcriptional programs mediating AML cell survival and proliferation.