chromosome-screening-human-preimplantation-embryos-using-spent

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

In clinical in vitro fertilization (IVF), the prevailing method for PGT-A requires biopsy of a few cells from the trophectoderm (TE). This is the lineage ...

non-invasive-chromosome-screening-nics-human-preimplantation-embryos

Non-invasive Chromosome Screening (NICS) of Human Preimplantation Embryos: Sample Collection and Chromosomal Ploidy Analysis by MALBAC-NGS

Chromosomal abnormalities are common in human embryos and cause implantation failure, early pregnancy losses, and birth defects in practice of assisted ...

Yikon genomics Co. Ltd.

Nowadays, most of the preimplantation genetic testing (PGT) is performed with a strategy of comprehensive chromosome screening and trophectoderm biopsy. Nevertheless, patients with ovarian insufficiency may not have competent blastocysts. In the present study, we aimed to establish the value of multiple annealing and looping-based amplification cycle (MALBAC)-based next-generation sequencing (NGS) for PGT in day-3 embryos. A total of 94.3% (1168/1239) of embryos yielded informative results, and the overall embryo euploid rate was 21.9% (256/1168). Overall, 225 embryos were transferred in 169 cycles with a clinical pregnancy rate of 49.1% (83/169). The live birth and implantation rates were 47.3% (80/169) and 44.4% (100/225), respectively. Double embryos transfer showed higher clinical pregnancy and live birth rates compared with single embryo transfer, but the implantation rates were similar (44.2% vs. 44.6%,  > 0.05). The euploid rate for reciprocal translocations (16.1%) was significantly lower than that for Robertsonian translocations (28.0%,  < 0.01) and inversions (28.0%,  < 0.01). However, higher percentages of embryos with  abnormalities were observed with Robertsonian translocations (23.3%,  < 0.01) and inversions (30.5%,  < 0.01) than with reciprocal translocations (11.6%). We demonstrated that NGS for PGT on day-3 embryos is an effective clinical application, particularly for patients with a diminished ovarian reserve and limited embryos.

A Rapid NGS-Based Preimplantation Genetic Testing for Chromosomal Abnormalities in Day-3 Blastomere Biopsy Allows Embryo Transfer Within the Same Treatment Cycle.

Article Total : 2	Year
Publication title	2021
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Jieliang Ma

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