Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
2.0K Views
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12:32 min
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September 7th, 2021
DOI :
September 7th, 2021
•Jin Huang*1,2,3,4, Yaxin Yao*5, Jialin Jia1,2,3,4, Xiaohui Zhu1,2,3,4, Jieliang Ma5, Jing Wang5, Ping Liu1,2,3,4, Sijia Lu5
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education, Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research, Yikon Genomics Co. Ltd.
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Chapters in this video
0:04
Introduction
1:24
Preparation and Denudation of Granulosa Cells, Oocyte Evaluation and Embryo Washing
3:15
Culture Medium Collection Options 1 and 2, Sample Collection
5:12
Culture Medium Lysis
6:22
Pre‐Library Preparation
7:24
Library Preparation
8:14
Library Purification and Quantification, Sequencing and Data Analysis
9:43
Results: Embryo Screening and Selection Using NICS from a Patient
11:08
Conclusion
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