Erwin London

Using a novel dual fluorescence quenching assay for measurement of tryptophan depth within lipid bilayers to determine hydrophobic alpha-helix locations within membranes.

Cumulative effects of amino acid substitutions and hydrophobic mismatch upon the transmembrane stability and conformation of hydrophobic alpha-helices.

Ceramide selectively displaces cholesterol from ordered lipid domains (rafts): implications for lipid raft structure and function.

Relationship between sterol/steroid structure and participation in ordered lipid domains (lipid rafts): implications for lipid raft structure and function.

Position and ionization state of Asp in the core of membrane-inserted alpha helices control both the equilibrium between transmembrane and nontransmembrane helix topography and transmembrane helix positioning.

Topography of diphtheria toxin A chain inserted into lipid vesicles.

Palmitoylation and intracellular domain interactions both contribute to raft targeting of linker for activation of T cells.

Behavior of diphtheria toxin T domain containing substitutions that block normal membrane insertion at Pro345 and Leu307: control of deep membrane insertion and coupling between deep insertion of hydrophobic subdomains.

How principles of domain formation in model membranes may explain ambiguities concerning lipid raft formation in cells.

Cholesterol precursors stabilize ordinary and ceramide-rich ordered lipid domains (lipid rafts) to different degrees. Implications for the Bloch hypothesis and sterol biosynthesis disorders.

Topography of the hydrophilic helices of membrane-inserted diphtheria toxin T domain: TH1-TH3 as a hydrophilic tether.

An amino acid "transmembrane tendency" scale that approaches the theoretical limit to accuracy for prediction of transmembrane helices: relationship to biological hydrophobicity.

Membrane topography of the hydrophobic anchor sequence of poliovirus 3A and 3AB proteins and the functional effect of 3A/3AB membrane association upon RNA replication.

The phenyltetraene lysophospholipid analog PTE-ET-18-OMe as a fluorescent anisotropy probe of liquid ordered membrane domains (lipid rafts) and ceramide-rich membrane domains.

Effect of ceramide N-acyl chain and polar headgroup structure on the properties of ordered lipid domains (lipid rafts).

Using model membrane-inserted hydrophobic helices to study the equilibrium between transmembrane and nontransmembrane states.

Effect of the structure of lipids favoring disordered domain formation on the stability of cholesterol-containing ordered domains (lipid rafts): identification of multiple raft-stabilization mechanisms.

Effect of sequence hydrophobicity and bilayer width upon the minimum length required for the formation of transmembrane helices in membranes.

The control of transmembrane helix transverse position in membranes by hydrophilic residues.

How interaction of perfringolysin O with membranes is controlled by sterol structure, lipid structure, and physiological low pH: insights into the origin of perfringolysin O-lipid raft interaction.

Detecting ordered domain formation (lipid rafts) in model membranes using Tempo.

Behavior of the deeply inserted helices in diphtheria toxin T domain: helices 5, 8, and 9 interact strongly and promote pore formation, while helices 6/7 limit pore formation.

Effect of lipid composition on the topography of membrane-associated hydrophobic helices: stabilization of transmembrane topography by anionic lipids.

Preparation and properties of asymmetric vesicles that mimic cell membranes: effect upon lipid raft formation and transmembrane helix orientation.

Strong correlation between statistical transmembrane tendency and experimental hydrophobicity scales for identification of transmembrane helices.

The membrane topography of the diphtheria toxin T domain linked to the a chain reveals a transient transmembrane hairpin and potential translocation mechanisms.

The effect of hydrophilic substitutions and anionic lipids upon the transverse positioning of the transmembrane helix of the ErbB2 (neu) protein incorporated into model membrane vesicles.

Low pH-induced pore formation by the T domain of botulinum toxin type A is dependent upon NaCl concentration.

Cholesterol lipids of Borrelia burgdorferi form lipid rafts and are required for the bactericidal activity of a complement-independent antibody.

Perfringolysin O association with ordered lipid domains: implications for transmembrane protein raft affinity.

Preparation and properties of asymmetric large unilamellar vesicles: interleaflet coupling in asymmetric vesicles is dependent on temperature but not curvature.

Measurement of lipid nanodomain (raft) formation and size in sphingomyelin/POPC/cholesterol vesicles shows TX-100 and transmembrane helices increase domain size by coalescing preexisting nanodomains but do not induce domain formation.

Anandamide externally added to lipid vesicles containing trapped fatty acid amide hydrolase (FAAH) is readily hydrolyzed in a sterol-modulated fashion.

The dependence of lipid asymmetry upon phosphatidylcholine acyl chain structure.

Altering hydrophobic sequence lengths shows that hydrophobic mismatch controls affinity for ordered lipid domains (rafts) in the multitransmembrane strand protein perfringolysin O.

Lipid exchange between Borrelia burgdorferi and host cells.

Sphingolipids and membrane domains: recent advances.

Proving lipid rafts exist: membrane domains in the prokaryote Borrelia burgdorferi have the same properties as eukaryotic lipid rafts.

Mapping peptide thiol accessibility in membranes using a quaternary ammonium isotope-coded mass tag (ICMT).

The dependence of lipid asymmetry upon polar headgroup structure.

Effect of cyclodextrin and membrane lipid structure upon cyclodextrin-lipid interaction.

Transmembrane protein (perfringolysin o) association with ordered membrane domains (rafts) depends upon the raft-associating properties of protein-bound sterol.

The influence of natural lipid asymmetry upon the conformation of a membrane-inserted protein (perfringolysin O).

Preparation of artificial plasma membrane mimicking vesicles with lipid asymmetry.

Selective association of outer surface lipoproteins with the lipid rafts of Borrelia burgdorferi.

Using Sterol Substitution to Probe the Role of Membrane Domains in Membrane Functions.

Decreasing Transmembrane Segment Length Greatly Decreases Perfringolysin O Pore Size.

Ordered raft domains induced by outer leaflet sphingomyelin in cholesterol-rich asymmetric vesicles.

Membrane fusion: A new role for lipid domains?

Raft-like membrane domains in pathogenic microorganisms.

Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism.

The Effect of Membrane Lipid Composition on the Formation of Lipid Ultrananodomains.

Cholesterol lipids and cholesterol-containing lipid rafts in bacteria.

Subnanometer Structure of an Asymmetric Model Membrane: Interleaflet Coupling Influences Domain Properties.

New Insights into How Cholesterol and Unsaturation Control Lipid Domain Formation.

Highly Hydrophilic Segments Attached to Hydrophobic Peptides Translocate Rapidly across Membranes.

Efficient replacement of plasma membrane outer leaflet phospholipids and sphingolipids in cells with exogenous lipids.

Ordered Membrane Domain-Forming Properties of the Lipids of Borrelia burgdorferi.

Islet Amyloid Polypeptide Membrane Interactions: Effects of Membrane Composition.

The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis.

Changes in glucosylceramide structure affect virulence and membrane biophysical properties of Cryptococcus neoformans.

Analysis of Lipids and Lipid Rafts in Borrelia.

Effects of host cell sterol composition upon internalization of and clustered β1 integrin.

Sterol Structure Strongly Modulates Membrane-Islet Amyloid Polypeptide Interactions.

Lipid rafts can form in the inner and outer membranes of Borrelia burgdorferi and have different properties and associated proteins.

Lipid Structure and Composition Control Consequences of Interleaflet Coupling in Asymmetric Vesicles.

Preparation of asymmetric phospholipid vesicles for use as cell membrane models.

Sterol structure dependence of insulin receptor and insulin-like growth factor 1 receptor activation.

Effect of sterol structure on ordered membrane domain (raft) stability in symmetric and asymmetric vesicles.

Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching.

Membrane Structure-Function Insights from Asymmetric Lipid Vesicles.

Helicobacter pylori lipids can form ordered membrane domains (rafts).

Kiss and Run Asymmetric Vesicles to Investigate Coupling.

Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.

Nanodomains can persist at physiologic temperature in plasma membrane vesicles and be modulated by altering cell lipids.

Induction of Ordered Lipid Raft Domain Formation by Loss of Lipid Asymmetry.

Sphingomyelins and ent-Sphingomyelins Form Homophilic Nano-Subdomains within Liquid Ordered Domains.

Preparation and Drug Entrapment Properties of Asymmetric Liposomes Containing Cationic and Anionic Lipids.

Preparation and utility of asymmetric lipid vesicles for studies of perfringolysin O-lipid interactions.

The Fluorescent Dye 1,6-Diphenyl-1,3,5-hexatriene Binds to Amyloid Fibrils Formed by Human Amylin and Provides a New Probe of Amylin Amyloid Kinetics.

Phospholipid exchange shows insulin receptor activity is supported by both the propensity to form wide bilayers and ordered raft domains.

Using cyclodextrin-induced lipid substitution to study membrane lipid and ordered membrane domain (raft) function in cells.

Preparation of Asymmetric Vesicles with Trapped CsCl Avoids Osmotic Imbalance, Non-Physiological External Solutions, and Minimizes Leakage.

Cholesterol and sphingomyelin are critical for Fcγ receptor-mediated phagocytosis of Cryptococcus neoformans by macrophages.

Loss of plasma membrane lipid asymmetry can induce ordered domain (raft) formation.

Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.

Molecular substructure of the liquid-ordered phase formed by sphingomyelin and cholesterol: sphingomyelin clusters forming nano-subdomains are a characteristic feature.

Ordered Domain (Raft) Formation in Asymmetric Vesicles and Its Induction upon Loss of Lipid Asymmetry in Artificial and Natural Membranes.

Impact of Ca on membrane catalyzed IAPP amyloid formation and IAPP induced vesicle leakage.

Lipid-driven interleaflet coupling of plasma membrane order regulates FcεRI signaling in mast cells.

Inner leaflet cationic lipid increases nucleic acid loading independently of outer leaflet lipid charge in asymmetric liposomes.

MαCD-based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activity.