Samuel H. Wilson

The domain organization and properties of individual domains of DNA topoisomerase V, a type 1B topoisomerase with DNA repair activities.

Loss of DNA polymerase beta stacking interactions with templating purines, but not pyrimidines, alters catalytic efficiency and fidelity.

DNA polymerase beta.

1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase beta.

Polymerase beta simulations suggest that Arg258 rotation is a slow step rather than large subdomain motions per se.

Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress.

Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen.

Binary system for selective photoaffinity labeling of base excision repair DNA polymerases.

Efficiency of correct nucleotide insertion governs DNA polymerase fidelity.

Rapid segmental and subdomain motions of DNA polymerase beta.

Mapping of the interaction interface of DNA polymerase beta with XRCC1.

Involvement of DNA polymerase beta in protection against the cytotoxicity of oxidative DNA damage.

Hypersensitivity of DNA polymerase beta null mouse fibroblasts reflects accumulation of cytotoxic repair intermediates from site-specific alkyl DNA lesions.

Structure of DNA polymerase beta with the mutagenic DNA lesion 8-oxodeoxyguanine reveals structural insights into its coding potential.

The Werner syndrome protein stimulates DNA polymerase beta strand displacement synthesis via its helicase activity.

DNA polymerase beta gene expression: the promoter activator CREB-1 is upregulated in Chinese hamster ovary cells by DNA alkylating agent-induced stress.

Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice.

Report on the First US-Japan DNA Repair Meeting. Sendai, Japan, October 27-31, 2002.

The base substitution fidelity of DNA polymerase beta-dependent single nucleotide base excision repair.

Structural insights into the origins of DNA polymerase fidelity.

Localization of the deoxyribose phosphate lyase active site in human DNA polymerase iota by controlled proteolysis.

Suppressed catalytic activity of base excision repair enzymes on rotationally positioned uracil in nucleosomes.

Base excision repair intermediates induce p53-independent cytotoxic and genotoxic responses.

Cloning and characterization of a novel member of the human ATF/CREB family: ATF2 deletion, a potential regulator of the human DNA polymerase beta promoter.

Base excision repair deficiency caused by polymerase beta haploinsufficiency: accelerated DNA damage and increased mutational response to carcinogens.

High-level expression and purification of untagged and histidine-tagged HIV-1 reverse transcriptase.

Recombinogenic phenotype of human activation-induced cytosine deaminase.

Shaping science policy in the age of genomics.

AP endonuclease and poly(ADP-ribose) polymerase-1 interact with the same base excision repair intermediate.

Influence of DNA structure on DNA polymerase beta active site function: extension of mutagenic DNA intermediates.

Highly organized but pliant active site of DNA polymerase beta: compensatory mechanisms in mutant enzymes revealed by dynamics simulations and energy analyses.

Increased postischemic brain injury in mice deficient in uracil-DNA glycosylase.

The Environmental Genome Project: phase I and beyond.

Critical role of magnesium ions in DNA polymerase beta's closing and active site assembly.

Dynamic characterization of a DNA repair enzyme: NMR studies of [methyl-13C]methionine-labeled DNA polymerase beta.

Identification of small molecule synthetic inhibitors of DNA polymerase beta by NMR chemical shift mapping.

AP endonuclease-independent DNA base excision repair in human cells.

In situ analysis of repair processes for oxidative DNA damage in mammalian cells.

Structural insights into DNA polymerase beta deterrents for misincorporation support an induced-fit mechanism for fidelity.

DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair.

Base excision repair in nucleosomes lacking histone tails.

Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure.

Human base excision repair enzymes apurinic/apyrimidinic endonuclease1 (APE1), DNA polymerase beta and poly(ADP-ribose) polymerase 1: interplay between strand-displacement DNA synthesis and proofreading exonuclease activity.

DNA polymerase lambda mediates a back-up base excision repair activity in extracts of mouse embryonic fibroblasts.

REV1 mediated mutagenesis in base excision repair deficient mouse fibroblast.

Involvement of poly(ADP-ribose) polymerase activity in regulating Chk1-dependent apoptotic cell death.

Personalized exposure assessment: promising approaches for human environmental health research.

DNA polymerase lambda protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair.

Strategic planning: establishing need and clarifying motivation.

Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate.

Structural insight into the DNA polymerase beta deoxyribose phosphate lyase mechanism.

Mismatch-induced conformational distortions in polymerase beta support an induced-fit mechanism for fidelity.

The need for exposure health sciences.

Framework for environmental exposure research: the disease-first approach.

Syn-full behavior by T7 DNA polymerase.

A thymine isostere in the templating position disrupts assembly of the closed DNA polymerase beta ternary complex.

The Werner syndrome protein operates in base excision repair and cooperates with DNA polymerase beta.

Structure and mechanism of DNA polymerase Beta.

Magnesium-induced assembly of a complete DNA polymerase catalytic complex.

Determination of lysine pK values using [5-13C]lysine: application to the lyase domain of DNA Pol beta.

Activities and mechanism of DNA polymerase beta.

Disease-first: a new paradigm for environmental health science research.

Haploinsufficiency in DNA polymerase beta increases cancer risk with age and alters mortality rate.

Energy analysis of chemistry for correct insertion by DNA polymerase beta.

NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells.

Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage.

Structure of DNA polymerase beta with a benzo[c]phenanthrene diol epoxide-adducted template exhibits mutagenic features.

Hypersensitivity phenotypes associated with genetic and synthetic inhibitor-induced base excision repair deficiency.

Down-regulation of DNA polymerase beta accompanies somatic hypermutation in human BL2 cell lines.

Magnesium-cationic dummy atom molecules enhance representation of DNA polymerase beta in molecular dynamics simulations: improved accuracy in studies of structural features and mutational effects.

Regulation of DNA repair fidelity by molecular checkpoints: "gates" in DNA polymerase beta's substrate selection.

Modifying the beta,gamma leaving-group bridging oxygen alters nucleotide incorporation efficiency, fidelity, and the catalytic mechanism of DNA polymerase beta.

ATR signaling mediates an S-phase checkpoint after inhibition of poly(ADP-ribose) polymerase activity.

Differing conformational pathways before and after chemistry for insertion of dATP versus dCTP opposite 8-oxoG in DNA polymerase beta.

Coordination of steps in single-nucleotide base excision repair mediated by apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta.

Interplay between DNA polymerases beta and lambda in repair of oxidation DNA damage in chicken DT40 cells.

OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells.

Ochratoxin A-induced mutagenesis in mammalian cells is consistent with the production of oxidative stress.

Different structural states in oligonucleosomes are required for early versus late steps of base excision repair.

The X family portrait: structural insights into biological functions of X family polymerases.

Exploring the role of large conformational changes in the fidelity of DNA polymerase beta.

Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress.

Comparative assessment of plasmid and oligonucleotide DNA substrates in measurement of in vitro base excision repair activity.

HMGB1 is a cofactor in mammalian base excision repair.

(R)-beta,gamma-fluoromethylene-dGTP-DNA ternary complex with DNA polymerase beta.

DNA polymerase beta fidelity: halomethylene-modified leaving groups in pre-steady-state kinetic analysis reveal differences at the chemical transition state.

XRCC1 and DNA polymerase beta in cellular protection against cytotoxic DNA single-strand breaks.

Incorrect nucleotide insertion at the active site of a G:A mismatch catalyzed by DNA polymerase beta.

DNA damage response protein ASCIZ links base excision repair with immunoglobulin gene conversion.

Negligible impact of pol iota expression on the alkylation sensitivity of pol beta-deficient mouse fibroblast cells.

Structures of DNA polymerase beta with active-site mismatches suggest a transient abasic site intermediate during misincorporation.

Folate deficiency induces neurodegeneration and brain dysfunction in mice lacking uracil DNA glycosylase.

Mutagenesis is elevated in male germ cells obtained from DNA polymerase-beta heterozygous mice.

Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition.

Alpha,beta-methylene-2'-deoxynucleoside 5'-triphosphates as noncleavable substrates for DNA polymerases: isolation, characterization, and stability studies of novel 2'-deoxycyclonucleosides, 3,5'-cyclo-dG, and 2,5'-cyclo-dT.

Human DNA polymerase theta possesses 5'-dRP lyase activity and functions in single-nucleotide base excision repair in vitro.

Alpha,beta-difluoromethylene deoxynucleoside 5'-triphosphates: a convenient synthesis of useful probes for DNA polymerase beta structure and function.

Characterization of DNA polymerase beta splicing variants in gastric cancer: the most frequent exon 2-deleted isoform is a non-coding RNA.

Gastrointestinal hyperplasia with altered expression of DNA polymerase beta.

Coordination between polymerase beta and FEN1 can modulate CAG repeat expansion.

A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases.

PARP inhibition during alkylation-induced genotoxic stress signals a cell cycle checkpoint response mediated by ATM.

DNA polymerase beta and PARP activities in base excision repair in living cells.

DNA polymerase beta substrate specificity: side chain modulation of the "A-rule".

DNA polymerase beta-dependent long patch base excision repair in living cells.

HMGB1: roles in base excision repair and related function.

FEN1 functions in long patch base excision repair under conditions of oxidative stress in vertebrate cells.

Halogenated beta,gamma-methylene- and ethylidene-dGTP-DNA ternary complexes with DNA polymerase beta: structural evidence for stereospecific binding of the fluoromethylene analogues.

DNA polymerase beta ribonucleotide discrimination: insertion, misinsertion, extension, and coding.

Mutagenic conformation of 8-oxo-7,8-dihydro-2'-dGTP in the confines of a DNA polymerase active site.

Alkylation DNA damage in combination with PARP inhibition results in formation of S-phase-dependent double-strand breaks.

DNA polymerase structure-based insight on the mutagenic properties of 8-oxoguanine.

Synthesis and biological evaluation of fluorinated deoxynucleotide analogs based on bis-(difluoromethylene)triphosphoric acid.

DNA polymerases beta and lambda mediate overlapping and independent roles in base excision repair in mouse embryonic fibroblasts.

Mutagenesis dependent upon the combination of activation-induced deaminase expression and a double-strand break.

Base excision repair and design of small molecule inhibitors of human DNA polymerase β.

Substrate channeling in mammalian base excision repair pathways: passing the baton.

Requirement for NBS1 in the S phase checkpoint response to DNA methylation combined with PARP inhibition.

Molecular insights into DNA polymerase deterrents for ribonucleotide insertion.

Single-nucleotide base excision repair DNA polymerase activity in C. elegans in the absence of DNA polymerase β.

Lucanthone and its derivative hycanthone inhibit apurinic endonuclease-1 (APE1) by direct protein binding.

Metal-induced DNA translocation leads to DNA polymerase conformational activation.

Binary complex crystal structure of DNA polymerase β reveals multiple conformations of the templating 8-oxoguanine lesion.

Increased PARP-1 association with DNA in alkylation damaged, PARP-inhibited mouse fibroblasts.

DNA base excision repair: a mechanism of trinucleotide repeat expansion.

Stereospecific formation of a ternary complex of (S)-α,β-fluoromethylene-dATP with DNA pol β.

β,γ-CHF- and β,γ-CHCl-dGTP diastereomers: synthesis, discrete 31P NMR signatures, and absolute configurations of new stereochemical probes for DNA polymerases.

HMGN1 protein regulates poly(ADP-ribose) polymerase-1 (PARP-1) self-PARylation in mouse fibroblasts.

Structures of dNTP intermediate states during DNA polymerase active site assembly.

DNA sequence context effects on the glycosylase activity of human 8-oxoguanine DNA glycosylase.

Pol β associated complex and base excision repair factors in mouse fibroblasts.

Effect of β,γ-CHF- and β,γ-CHCl-dGTP halogen atom stereochemistry on the transition state of DNA polymerase β.

DNA polymerase β gap-filling translesion DNA synthesis.

Identification of one of the apurinic/apyrimidinic lyase active sites of topoisomerase V by structural and functional studies.

Evidence for abasic site sugar phosphate-mediated cytotoxicity in alkylating agent treated Saccharomyces cerevisiae.

Hyperactivation of PARP triggers nonhomologous end-joining in repair-deficient mouse fibroblasts.

Predicting enhanced cell killing through PARP inhibition.

DNA polymerase minor groove interactions modulate mutagenic bypass of a templating 8-oxoguanine lesion.

Perspective: pre-chemistry conformational changes in DNA polymerase mechanisms.

Amino acid substitution in the active site of DNA polymerase β explains the energy barrier of the nucleotidyl transfer reaction.

Insights into the conformation of aminofluorene-deoxyguanine adduct in a DNA polymerase active site.

Interaction between DNA Polymerase β and BRCA1.

Observing a DNA polymerase choose right from wrong.

Preventing oxidation of cellular XRCC1 affects PARP-mediated DNA damage responses.

Understanding the loss-of-function in a triple missense mutant of DNA polymerase β found in prostate cancer.

Structure and mechanism of DNA polymerase β.

Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair.

The dark side of DNA repair.

Phylogenetic analysis and evolutionary origins of DNA polymerase X-family members.

Uncovering the polymerase-induced cytotoxicity of an oxidized nucleotide.

Structures of human DNA polymerases ν and θ expose their end game.

Two Scaffolds from Two Flips: (α,β)/(β,γ) CH2/NH "Met-Im" Analogues of dTTP.

New structural snapshots provide molecular insights into the mechanism of high fidelity DNA synthesis.

Intrinsic mutagenic properties of 5-chlorocytosine: A mechanistic connection between chronic inflammation and cancer.

Requirement for transient metal ions revealed through computational analysis for DNA polymerase going in reverse.

Capturing snapshots of APE1 processing DNA damage.

Oxidized dNTPs and the OGG1 and MUTYH DNA glycosylases combine to induce CAG/CTG repeat instability.

Insertion of oxidized nucleotide triggers rapid DNA polymerase opening.

Combined Effects of High-Dose Bisphenol A and Oxidizing Agent (KBrO3) on Cellular Microenvironment, Gene Expression, and Chromatin Structure of Ku70-deficient Mouse Embryonic Fibroblasts.

Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells.

Structures of DNA Polymerase Mispaired DNA Termini Transitioning to Pre-catalytic Complexes Support an Induced-Fit Fidelity Mechanism.

Impact of Ribonucleotide Backbone on Translesion Synthesis and Repair of 7,8-Dihydro-8-oxoguanine.

DNA polymerase β contains a functional nuclear localization signal at its N-terminus.

Hiding in Plain Sight: The Bimetallic Magnesium Covalent Bond in Enzyme Active Sites.

Oxidized nucleotide insertion by pol β confounds ligation during base excision repair.

Revealing the role of the product metal in DNA polymerase β catalysis.

DNA polymerase β uses its lyase domain in a processive search for DNA damage.

Time-lapse crystallography snapshots of a double-strand break repair polymerase in action.

Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair.

Complementation of aprataxin deficiency by base excision repair enzymes in mitochondrial extracts.

Role of DNA polymerase β oxidized nucleotide insertion in DNA ligation failure.

DNA polymerase β: A missing link of the base excision repair machinery in mammalian mitochondria.

XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity.

Transcriptional mutagenesis mediated by 8-oxoG induces translational errors in mammalian cells.

Mapping Functional Substrate-Enzyme Interactions in the pol β Active Site through Chemical Biology: Structural Responses to Acidity Modification of Incoming dNTPs.

Probing DNA Base-Dependent Leaving Group Kinetic Effects on the DNA Polymerase Transition State.

Transitions in DNA polymerase β μs-ms dynamics related to substrate binding and catalysis.

DNA scanning by base excision repair enzymes and implications for pathway coordination.

Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate.

Repair pathway for PARP-1 DNA-protein crosslinks.

A Transition-State Perspective on Y-Family DNA Polymerase η Fidelity in Comparison with X-Family DNA Polymerases λ and β.

Molecular basis for the faithful replication of 5-methylcytosine and its oxidized forms by DNA polymerase β.

Eukaryotic Base Excision Repair: New Approaches Shine Light on Mechanism.

The Pol β variant containing exon α is deficient in DNA polymerase but has full dRP lyase activity.

DNA polymerase beta and other gap-filling enzymes in mammalian base excision repair.

Shining light on the response to repair intermediates in DNA of living cells.

DNA polymerase β nucleotide-stabilized template misalignment fidelity depends on local sequence context.

Oxidative DNA-protein crosslinks formed in mammalian cells by abasic site lyases involved in DNA repair.

Oxidative DNA Damage Modulates DNA Methylation Pattern in Human Breast Cancer 1 (BRCA1) Gene via the Crosstalk between DNA Polymerase β and a DNA Methyltransferase.

Revealing an Internal Stabilization Deficiency in the DNA Polymerase β K289M Cancer Variant through the Combined Use of Chemical Biology and X-ray Crystallography.

WITHDRAWN: Requirements for PARP-1 covalent crosslinking to DNA (PARP-1 DPC).

Mitochondrial dysfunction and DNA damage accompany enhanced levels of formaldehyde in cultured primary human fibroblasts.

Requirements for PARP-1 covalent crosslinking to DNA (PARP-1 DPC).

Structure of a DNA polymerase abortive complex with the 8OG:dA base pair at the primer terminus.

Preferential DNA Polymerase β Reverse Reaction with Imidodiphosphate.

Lysines in the lyase active site of DNA polymerase β destabilize nonspecific DNA binding, facilitating searching and DNA gap recognition.

Watching a double strand break repair polymerase insert a pro-mutagenic oxidized nucleotide.

Perspectives on formaldehyde dysregulation: Mitochondrial DNA damage and repair in mammalian cells.

Structural basis for proficient oxidized ribonucleotide insertion in double strand break repair.

Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints.