William A. Beard

Loss of DNA polymerase beta stacking interactions with templating purines, but not pyrimidines, alters catalytic efficiency and fidelity.

Polymerase beta simulations suggest that Arg258 rotation is a slow step rather than large subdomain motions per se.

Efficiency of correct nucleotide insertion governs DNA polymerase fidelity.

Rapid segmental and subdomain motions of DNA polymerase beta.

Structure of DNA polymerase beta with the mutagenic DNA lesion 8-oxodeoxyguanine reveals structural insights into its coding potential.

Structural insights into the origins of DNA polymerase fidelity.

Localization of the deoxyribose phosphate lyase active site in human DNA polymerase iota by controlled proteolysis.

High-level expression and purification of untagged and histidine-tagged HIV-1 reverse transcriptase.

Influence of DNA structure on DNA polymerase beta active site function: extension of mutagenic DNA intermediates.

Highly organized but pliant active site of DNA polymerase beta: compensatory mechanisms in mutant enzymes revealed by dynamics simulations and energy analyses.

Critical role of magnesium ions in DNA polymerase beta's closing and active site assembly.

Dynamic characterization of a DNA repair enzyme: NMR studies of [methyl-13C]methionine-labeled DNA polymerase beta.

Structural insights into DNA polymerase beta deterrents for misincorporation support an induced-fit mechanism for fidelity.

DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair.

DNA polymerase lambda mediates a back-up base excision repair activity in extracts of mouse embryonic fibroblasts.

Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate.

Structural insight into the DNA polymerase beta deoxyribose phosphate lyase mechanism.

Mismatch-induced conformational distortions in polymerase beta support an induced-fit mechanism for fidelity.

Syn-full behavior by T7 DNA polymerase.

A thymine isostere in the templating position disrupts assembly of the closed DNA polymerase beta ternary complex.

Structure and mechanism of DNA polymerase Beta.

Magnesium-induced assembly of a complete DNA polymerase catalytic complex.

Determination of lysine pK values using [5-13C]lysine: application to the lyase domain of DNA Pol beta.

Activities and mechanism of DNA polymerase beta.

Energy analysis of chemistry for correct insertion by DNA polymerase beta.

Structure of DNA polymerase beta with a benzo[c]phenanthrene diol epoxide-adducted template exhibits mutagenic features.

Magnesium-cationic dummy atom molecules enhance representation of DNA polymerase beta in molecular dynamics simulations: improved accuracy in studies of structural features and mutational effects.

Regulation of DNA repair fidelity by molecular checkpoints: "gates" in DNA polymerase beta's substrate selection.

Modifying the beta,gamma leaving-group bridging oxygen alters nucleotide incorporation efficiency, fidelity, and the catalytic mechanism of DNA polymerase beta.

Differing conformational pathways before and after chemistry for insertion of dATP versus dCTP opposite 8-oxoG in DNA polymerase beta.

Coordination of steps in single-nucleotide base excision repair mediated by apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta.

The X family portrait: structural insights into biological functions of X family polymerases.

Exploring the role of large conformational changes in the fidelity of DNA polymerase beta.

(R)-beta,gamma-fluoromethylene-dGTP-DNA ternary complex with DNA polymerase beta.

DNA polymerase beta fidelity: halomethylene-modified leaving groups in pre-steady-state kinetic analysis reveal differences at the chemical transition state.

Incorrect nucleotide insertion at the active site of a G:A mismatch catalyzed by DNA polymerase beta.

Negligible impact of pol iota expression on the alkylation sensitivity of pol beta-deficient mouse fibroblast cells.

Structures of DNA polymerase beta with active-site mismatches suggest a transient abasic site intermediate during misincorporation.

Alpha,beta-methylene-2'-deoxynucleoside 5'-triphosphates as noncleavable substrates for DNA polymerases: isolation, characterization, and stability studies of novel 2'-deoxycyclonucleosides, 3,5'-cyclo-dG, and 2,5'-cyclo-dT.

Alpha,beta-difluoromethylene deoxynucleoside 5'-triphosphates: a convenient synthesis of useful probes for DNA polymerase beta structure and function.

Coordination between polymerase beta and FEN1 can modulate CAG repeat expansion.

A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases.

DNA polymerase beta and PARP activities in base excision repair in living cells.

DNA polymerase beta substrate specificity: side chain modulation of the "A-rule".

Halogenated beta,gamma-methylene- and ethylidene-dGTP-DNA ternary complexes with DNA polymerase beta: structural evidence for stereospecific binding of the fluoromethylene analogues.

DNA polymerase beta ribonucleotide discrimination: insertion, misinsertion, extension, and coding.

Mutagenic conformation of 8-oxo-7,8-dihydro-2'-dGTP in the confines of a DNA polymerase active site.

DNA polymerase structure-based insight on the mutagenic properties of 8-oxoguanine.

Synthesis and biological evaluation of fluorinated deoxynucleotide analogs based on bis-(difluoromethylene)triphosphoric acid.

Base excision repair and design of small molecule inhibitors of human DNA polymerase β.

Substrate channeling in mammalian base excision repair pathways: passing the baton.

Molecular insights into DNA polymerase deterrents for ribonucleotide insertion.

Metal-induced DNA translocation leads to DNA polymerase conformational activation.

Binary complex crystal structure of DNA polymerase β reveals multiple conformations of the templating 8-oxoguanine lesion.

Stereospecific formation of a ternary complex of (S)-α,β-fluoromethylene-dATP with DNA pol β.

Structures of dNTP intermediate states during DNA polymerase active site assembly.

DNA sequence context effects on the glycosylase activity of human 8-oxoguanine DNA glycosylase.

Effect of β,γ-CHF- and β,γ-CHCl-dGTP halogen atom stereochemistry on the transition state of DNA polymerase β.

DNA polymerase β gap-filling translesion DNA synthesis.

DNA polymerase minor groove interactions modulate mutagenic bypass of a templating 8-oxoguanine lesion.

Perspective: pre-chemistry conformational changes in DNA polymerase mechanisms.

Amino acid substitution in the active site of DNA polymerase β explains the energy barrier of the nucleotidyl transfer reaction.

Insights into the conformation of aminofluorene-deoxyguanine adduct in a DNA polymerase active site.

Observing a DNA polymerase choose right from wrong.

Understanding the loss-of-function in a triple missense mutant of DNA polymerase β found in prostate cancer.