Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

13.2K Views

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11:42 min

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August 21st, 2017

DOI :

10.3791/55237-v

August 21st, 2017

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María Jesús Sánchez-Calabuig¹^,², Francisco Alberto García-Vázquez³, Ricardo Laguna-Barraza¹, Carlos Barros-García⁴, Daniel García-Parraga⁵, Dimitrios Rizos¹, Alfonso Gutiérrez Adan¹, José Félix Pérez-Gutíerrez²

¹Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), ²Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, ³Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, ⁴Mundomar, Benidorm, ⁵Veterinary Services, L'Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013

Summary

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Keywords Bottlenose Dolphin

Spermatozoa

Cryopreservation

Heterologous In Vitro Fertilization

Liquid Nitrogen Vapor

Assisted Reproductive Technologies

Male Pronuclear Formation

Hybrid Embryo Cleavage

Chapters in this video

0:05

Title

1:26

Dolphin Sperm Collection

2:44

Dolphin Sperm Evaluation and Cryopreservation

4:20

Heterologous In Vitro Fertilization Using Zona-intact Bovine Oocytes: Set-up

7:23

Heterologous In Vitro Fertilization Using Zona-intact Bovine Oocytes

9:01

Results: Analysis of Frozen Dolphin Spermatozoa and Dolphin-bovine Hybrid Embryos

10:31

Conclusion

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