In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

8.2K Views

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10:48 min

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December 17th, 2017

DOI :

10.3791/55641-v

December 17th, 2017

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Shen Zhang¹, Yonggen Wu¹, Yimin Weng², Zhihui Xu¹, Wenmin Chen³, Dahan Zheng⁴, Wei Lin⁵, Jun Liu⁶, Ying Zhou¹^,⁷

¹Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, ²Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, ³Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, ⁴School of Laboratory Medicine and Life Science, Wenzhou Medical University, ⁵School of Pharmaceutical Science, Wenzhou Medical University, ⁶Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, ⁷Department of Histology and Embryology, Wenzhou Medical University

Summary

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Keywords Mouse Preantral Follicles

In Vitro Growth

Simulated Microgravity

Follicle Isolation

Follicle Encapsulation

Alginate Beads

Rotating Culture System

In Vitro Culture

Control Group

Microgravity Treatment

Chapters in this video

0:00

Title

1:15

Follicle Isolation and Encapsulation

4:29

In Vitro Culture of Encapsulated Follicles Under Simulated Microgravity Treatment

8:28

Changing Culture Medium

9:17

Follicle Retrieval

9:49

Results

10:24

Conclusion

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