Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

10.9K Views

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11:52 min

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August 3rd, 2017

DOI :

10.3791/55700-v

August 3rd, 2017

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Bao Li Zhang¹^,²^,³, Da Yuan Gao¹^,²^,³, Xiao Xu Zhang¹^,³, Shuo Shi⁴, Winnie Shum¹

¹School of Life Science and Technology, ShanghaiTech University, ²Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, ³University of Chinese Academy of Sciences, ⁴Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

Summary

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Keywords Whole cell Patch clamp

Primary Epithelial Cells

Electrical Properties

Collagenase

Cell Dissociation

Chapters in this video

0:05

Title

0:59

Preparation of Dissection Instruments and Reagents

2:10

Isolation of Epithelial Cells from Rat Cauda Epididymides

6:09

Patch-clamp Experiment

8:32

Results: Typical Whole-cell Curent in Single Epididymal Principal Cells

10:03

Conclusion

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