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08:00 min
October 16th, 2008
DOI :
10.3791/936-v
Chapters
0:00
Title
0:49
Introduction
1:35
Removing the Vitelline Membrane
3:33
Gigaseal Formation
6:05
Outside-Out Topology
6:59
Conclusion
Summary
This is intended as an introduction to patch clamp recording from Xenopus laevis oocytes. It covers vitelline membrane removal, formation of a gigaohm seal (gigaseal), and the optional conversion of the patch to the outside-out topology.
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