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Mouse Heart and Skeletal Muscle Digestion for Fibro-Adipogenic Progenitor Culture

November 15th 2024



Summary

This protocol outlines the isolation of skeletal and cardiac muscle fibro-adipogenic progenitors (FAPs) from spiny mouse (Acomys) via enzymatic dissociation and fluorescence-activated cell sorting. The FAPs obtained from this protocol can be effectively expanded and differentiated to myofibroblasts and adipocytes.

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