JoVE Journal

Developmental Biology

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Fibro-Adipogenic Progenitor Isolation, Expansion, and Differentiation from the Spiny Mouse Model

This protocol outlines the isolation of skeletal and cardiac muscle fibro-adipogenic progenitors (FAPs) from spiny mouse (Acomys) via enzymatic dissociation and fluorescence-activated cell sorting. The FAPs obtained from this protocol can be effectively expanded and differentiated to myofibroblasts and adipocytes.

Chapters in this video

0:00

Introduction

1:13

Collection of Skeletal and Cardiac Muscles from Spiny Mouse for Isolating Fibro‐Adipogenic Progenitors (FAPs)

2:46

Digestion of Mouse Skeletal and Cardiac Muscles for Fluorescence‐Activated Cell Sorting and Culture of Fibro‐Adipogenic Progenitors (FAPs)

5:01

Fluorescence‐Activated Cell Sorting and Culture of Fibro‐Adipogenic Progenitors (FAPs)

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