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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

DOI :

10.3791/60310-v

October 15th, 2019

October 15th, 2019

15,725 Views

1Scripps Research, Department of Molecular Medicine

Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.

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Keywords Primary Myoblasts

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