Andreas Bechthold

Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A.

Engineered urdamycin glycosyltransferases are broadened and altered in substrate specificity.

Rationally designed glycosylated premithramycins: hybrid aromatic polyketides using genes from three different biosynthetic pathways.

Urdamycin L: a novel metabolic shunt product that provides evidence for the role of the urdM gene in the urdamycin A biosynthetic pathway of Streptomyces fradiae TU 2717.

The C-Glycosyltransferase UrdGT2 is unselective toward d- and l-configured nucleotide-bound rhodinoses.

Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes.

The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose.

Expression of the landomycin biosynthetic gene cluster in a PKS mutant of Streptomyces fradiae is dependent on the coexpression of a putative transcriptional activator gene.

Rational saccharide extension by using the natural product glycosyltransferase LanGT4.

The glycosyltransferase UrdGT2 catalyzes both C- and O-glycosidic sugar transfers.

Generation of Streptomyces globisporus SMY622 strain with increased landomycin E production and it's initial characterization.

Novel avilamycin derivatives with improved polarity generated by targeted gene disruption.

Function of lanGT3, a glycosyltransferase gene involved in landomycin A biosynthesis.

Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes.

Genes involved in formation and attachment of a two-carbon chain as a component of eurekanate, a branched-chain sugar moiety of avilamycin A.

Identification of the function of gene lndM2 encoding a bifunctional oxygenase-reductase involved in the biosynthesis of the antitumor antibiotic landomycin E by Streptomyces globisporus 1912 supports the originally assigned structure for landomycinone.

Novel natural compounds obtained by genome-based screening and genetic engineering.

Exploiting Pseudomonas putida for drug development.

Generation of novel landomycins M and O through targeted gene disruption.

LanGT2 Catalyzes the First Glycosylation Step during landomycin A biosynthesis.

It works: combinatorial biosynthesis for generating novel glycosylated compounds.

Genes encoding enzymes responsible for biosynthesis of L-lyxose and attachment of eurekanate during avilamycin biosynthesis.

LanV, a bifunctional enzyme: aromatase and ketoreductase during landomycin A biosynthesis.

A putative proteinase gene is involved in regulation of landomycin E biosynthesis in Streptomyces globisporus 1912.

The active conformation of avilamycin A is conferred by AviX12, a radical AdoMet enzyme.

Genes and enzymes involved in caffeic acid biosynthesis in the actinomycete Saccharothrix espanaensis.

Biosynthesis of the terpene phenalinolactone in Streptomyces sp. Tü6071: analysis of the gene cluster and generation of derivatives.

Glycosyltransferases involved in the biosynthesis of biologically active natural products that contain oligosaccharides.

On the acceptor substrate of C-glycosyltransferase UrdGT2: three prejadomycin C-Glycosides from an engineered mutant of Streptomyces globisporus 1912 DeltalndE(urdGT2).

Generation of new landomycins with altered saccharide patterns through over-expression of the glycosyltransferase gene lanGT3 in the biosynthetic gene cluster of landomycin A in Streptomyces cyanogenus S-136.

Type II polyketide synthases: gaining a deeper insight into enzymatic teamwork.

Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase.

A genomic screening approach to the structure-guided identification of drug candidates from natural sources.

SorF: a glycosyltransferase with promiscuous donor substrate specificity in vitro.

A strategy for cloning glycosyltransferase genes involved in natural product biosynthesis.

Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin.

The future of natural products as a source of new antibiotics.

Function of lanI in regulation of landomycin A biosynthesis in Streptomyces cyanogenus S136 and cross-complementation studies with Streptomyces antibiotic regulatory proteins encoding genes.

Heterologous expression and genetic engineering of the phenalinolactone biosynthetic gene cluster by using red/ET recombineering.

Functional expression of the Cre recombinase in actinomycetes.

Glycosyltransferases, important tools for drug design.

Deoxysugars in bioactive natural products: development of novel derivatives by altering the sugar pattern.

Coordination of export and glycosylation of landomycins in Streptomyces cyanogenus S136.

Marker removal from actinomycetes genome using Flp recombinase.

Aranciamycin analogs generated by combinatorial biosynthesis show improved antitumor activity.

Features and applications of bacterial glycosyltransferases: current state and prospects.

Engineering a function into a glycosyltransferase.

Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin.

Genes and enzymes involved in bacterial isoprenoid biosynthesis.

Differences in the substrate specificity of glycosyltransferases involved in landomycins A and E biosynthesis.

Chapter 12. The power of glycosyltransferases to generate bioactive natural compounds.

Cloning and sequencing of the biosynthetic gene cluster for saquayamycin Z and galtamycin B and the elucidation of the assembly of their saccharide chains.

A bacterial glycosyltransferase gene toolbox: generation and applications.

Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tü117.

Production of avilamycin A is regulated by AviC1 and AviC2, two transcriptional activators.

Functions of genes and enzymes involved in phenalinolactone biosynthesis.

Combinatorial and synthetic biosynthesis in actinomycetes.

Chemoenzymatic and bioenzymatic synthesis of carbohydrate containing natural products.

Rational design of an aryl-C-glycoside catalyst from a natural product O-glycosyltransferase.

Genome sequence of Streptomyces sp. strain Tü6071.

Cloning and heterologous expression of three type II PKS gene clusters from Streptomyces bottropensis.

SimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors.

Site-specific recombination strategies for engineering actinomycete genomes.

SnoaW/SnoaL2: a different two-component monooxygenase.

Complete genome sequence of Saccharothrix espanaensis DSM 44229(T) and comparison to the other completely sequenced Pseudonocardiaceae.

A transposon-based strategy to identify the regulatory gene network responsible for landomycin E biosynthesis.

Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.

Development of intergeneric conjugal gene transfer system in Streptomyces diastatochromogenes 1628 and its application for improvement of toyocamycin production.

A cryptic polyene biosynthetic gene cluster in Streptomyces calvus is expressed upon complementation with a functional bldA gene.

Pleiotropic regulatory genes bldA, adpA and absB are implicated in production of phosphoglycolipid antibiotic moenomycin.

Overexpression of ribosome recycling factor is responsible for improvement of nucleotide antibiotic-toyocamycin in Streptomyces diastatochromogenes 1628.

Insights into the bioactivity of mensacarcin and epoxide formation by MsnO8.

Complete genome sequence of producer of the glycopeptide antibiotic Aculeximycin Kutzneria albida DSM 43870T, a representative of minor genus of Pseudonocardiaceae.

Structural characterization of O- and C-glycosylating variants of the landomycin glycosyltransferase LanGT2.

Functional characterization of different ORFs including luciferase-like monooxygenase genes from the mensacarcin gene cluster.

The Gene bldA, a regulator of morphological differentiation and antibiotic production in streptomyces.

Changing Biosynthetic Profiles by Expressing bldA in Streptomyces Strains.

Minimum Information about a Biosynthetic Gene cluster.

Biosynthesis of the Fluorinated Natural Product Nucleocidin in Streptomyces calvus Is Dependent on the bldA-Specified Leu-tRNA(UUA) Molecule.

Bacillus cereus efflux protein BC3310 - a multidrug transporter of the unknown major facilitator family, UMF-2.

New Simocyclinones: Surprising Evolutionary and Biosynthetic Insights.

StreptomeDB 2.0--an extended resource of natural products produced by streptomycetes.

Characterization of representative rpoB gene mutations leading to a significant change in toyocamycin production of Streptomyces diastatochromogenes 1628.

An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host.

Thailandins A and B, New Polyene Macrolactone Compounds Isolated from Actinokineospora bangkokensis Strain 44EHW(T), Possessing Antifungal Activity against Anthracnose Fungi and Pathogenic Yeasts.

The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units.

Selection of an efficient promoter and its application in toyocamycin production improvement in Streptomyces diastatochromogenes 1628.