University of Greifswald

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis

Tobias Traeger^*1, Pia Koerner^*1, Wolfram Kessler¹, Katharina Cziupka¹, Stephan Diedrich¹, Alexandra Busemann¹, Claus-Dieter Heidecke¹, Stefan Maier¹

¹Department of Surgery, University of Greifswald

The Colon Ascendens Stent Peritonitis (CASP) is a highly standardized model for polymicrobial abdominal sepsis in rodents. This article describes the surgical procedure of CASP. The CASP model and its variants allow the systematic investigation of various problems concerning the subject of sepsis.

Immunology and Infection

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Malek Saleh¹, Mohammed R. Abdullah¹, Christian Schulz¹, Thomas Kohler¹, Thomas Pribyl¹, Inga Jensch¹, Sven Hammerschmidt¹

¹Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald

Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.

Medicine

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice

Annabel Kleinwort¹, Paula Döring², Christine Hackbarth¹, Maciej Patrzyk¹, Claus-Dieter Heidecke¹, Tobias Schulze¹

¹Department of General Surgery, Visceral, Thoracic and Vascular Surgery, Universitätsmedizin Greifswald, ²Institute of Pathology, Universitätsmedizin Greifswald

Murine distal colostomy provides a murine model for human diversion colitis, a predominantly lymphocytic colitis in colon segment excluded from the fecal stream.

Medicine

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Ruibing Xia^1,2,3, Simone Loy¹, Stefan Kääb^1,3, Anna Titova¹, Christian Schulz^1,2,3, Steffen Massberg^1,2,3, Sebastian Clauss^1,2,3

¹University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), ²Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), ³German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

Biology

Design and Implementation of a Rat Ex Vivo Lung Perfusion Model

William J. Cleveland^*1, Josephine E. Hees^*1,2, Claudius Balzer^1,2, Hunter F. Douglas¹, Troy Stevens^3,4,5, Matthias L. Riess^1,6,7

¹Anesthesiology, Vanderbilt University Medical Center, ²Anesthesiology, University of Greifswald, ³Department of Physiology and Cell Biology, University of South Alabama, ⁴Department of Medicine, University of South Alabama, ⁵Center for Lung Biology, University of South Alabama, ⁶Anesthesiology, Tennessee Valley Healthcare System Veterans Affairs Medical Center, ⁷Pharmacology, Vanderbilt University

Ex vivo lungs are useful for a variety of experiments to collect physiological data while excluding the confounding variables of in vivo experiments. Commercial setups are often expensive and limited in the types of data they can collect. We describe a method for building a fully modular setup, adaptable for various study designs.