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University of Greifswald

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis
Tobias Traeger *1, Pia Koerner *1, Wolfram Kessler 1, Katharina Cziupka 1, Stephan Diedrich 1, Alexandra Busemann 1, Claus-Dieter Heidecke 1, Stefan Maier 1
1Department of Surgery, University of Greifswald

The Colon Ascendens Stent Peritonitis (CASP) is a highly standardized model for polymicrobial abdominal sepsis in rodents. This article describes the surgical procedure of CASP. The CASP model and its variants allow the systematic investigation of various problems concerning the subject of sepsis.

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Immunology and Infection

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
Malek Saleh 1, Mohammed R. Abdullah 1, Christian Schulz 1, Thomas Kohler 1, Thomas Pribyl 1, Inga Jensch 1, Sven Hammerschmidt 1
1Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald

Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.

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Medicine

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice
Annabel Kleinwort 1, Paula Döring 2, Christine Hackbarth 1, Maciej Patrzyk 1, Claus-Dieter Heidecke 1, Tobias Schulze 1
1Department of General Surgery, Visceral, Thoracic and Vascular Surgery, Universitätsmedizin Greifswald, 2Institute of Pathology, Universitätsmedizin Greifswald

Murine distal colostomy provides a murine model for human diversion colitis, a predominantly lymphocytic colitis in colon segment excluded from the fecal stream.

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Medicine

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
Ruibing Xia 1,2,3, Simone Loy 1, Stefan Kääb 1,3, Anna Titova 1, Christian Schulz 1,2,3, Steffen Massberg 1,2,3, Sebastian Clauss 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

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Biology

Design and Implementation of a Rat Ex Vivo Lung Perfusion Model
William J. Cleveland *1, Josephine E. Hees *1,2, Claudius Balzer 1,2, Hunter F. Douglas 1, Troy Stevens 3,4,5, Matthias L. Riess 1,6,7
1Anesthesiology, Vanderbilt University Medical Center, 2Anesthesiology, University of Greifswald, 3Department of Physiology and Cell Biology, University of South Alabama, 4Department of Medicine, University of South Alabama, 5Center for Lung Biology, University of South Alabama, 6Anesthesiology, Tennessee Valley Healthcare System Veterans Affairs Medical Center, 7Pharmacology, Vanderbilt University

Ex vivo lungs are useful for a variety of experiments to collect physiological data while excluding the confounding variables of in vivo experiments. Commercial setups are often expensive and limited in the types of data they can collect. We describe a method for building a fully modular setup, adaptable for various study designs.

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