Loma Linda University School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Medicine

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Paul R. Krafft¹, William B. Rolland¹, Kamil Duris¹, Tim Lekic¹, Aaron Campbell², Jiping Tang¹, John H. Zhang^1,3,4

¹Department of Physiology and Pharmacology, Loma Linda University School of Medicine, ²College of Natural and Agricultural Sciences, University of California, Riverside , ³Department of Anesthesiology, Loma Linda University School of Medicine, ⁴Department of Neurosurgery, Loma Linda University School of Medicine

Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.

Neuroscience

A Pediatric Concussion Model in Mice: Closed Head Injury with Long-Term Disorders (CHILD)

Christophe J. Dubois^1,2, J. Yan³, A. Obenaus^*3, J. Badaut^*1,2,4

¹Centre d'Études Biologiques de Chizé-Centre National de la Recherche Scientifique (CEBC-CNRS), UMR 7372 CNRS/Université de La Rochelle, ²Univ. Bordeaux, CNRS, CRMSB, UMR 5536, ³Division of Biomedical Sciences, School of Medicine, University of California Riverside, ⁴Department of Basic Sciences, Loma Linda University School of Medicine

Pediatric concussions can result in long-term sequelae with physiological and psychological perturbations. This pediatric concussion mouse model results in long-term neuroinflammation, white matter alterations, and neuronal modifications with behavioral dysfunction. The model can be adapted to differential impact locations or repeated concussions to assess lifetime changes.