Odense University Hospital

10 ARTICLES PUBLISHED IN JoVE

Medicine

Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model

Travis K. Mann-Gow^*1, Troy R. Larson^*1, Chrissie T. Wøien², Thomas M. Andersen², Karl-Erik Andersson^3,4, Peter Zvara^1,2

¹Department of Surgery, University of Vermont, ²Department of Urology and Biomedical Laboratory, University of Southern Denmark, ³Wake Forest Institute for Regenerative Medicine, Wake Forest University, ⁴Institute for Clinical Medicine, Aarhus University

This study describes the surgical procedures and experimental techniques for performing awake cystometry in a freely moving mouse. In addition, it provides experimental evidence to support its optimization and standardization.

Medicine

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

Morten Hox¹, Travis Mann-Gow², Lars Lund^3,4, Peter Zvara^1,4

¹Biomedical Laboratory and the Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, ²Department of Surgery, University of Vermont, ³Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, ⁴Department of Urology, Odense University Hospital

This study describes a simplified surgical procedure and technique for performing cavernous nerve stimulation with the isolation of the nerve-electrode complex using silicone glue and intracavernous pressure measurement.

JoVE Journal

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

Maria Bloksgaard¹, Bjarne Thorsted², Jonathan R. Brewer², Jo G. R. De Mey^1,3

¹Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, ²Department of Biochemistry and Molecular Biology, University of Southern Denmark, ³Department of Cardiac, Thoracic and Vascular Surgery, Odense University Hospital

We describe simultaneous mechanical testing and 3D-imaging of the arterial wall of isolated, live human resistance arteries, and Fiji and Ilastik image analyses for the quantification of elastin and collagen spatial organization and volume densities. We discuss the use of these data in mathematical models of arterial wall mechanics.

Medicine

Isolation of Adipose Derived Regenerative Cells for the Treatment of Erectile Dysfunction Following Radical Prostatectomy

Sabrina T. Hansen^1,2, Charlotte H. Jensen^2,3,4, Jens A. Sørensen^2,4,5, Søren P. Sheikh^2,3,4, Lars Lund^1,2,4

¹Department of Urology, Odense University Hospital, ²Clinical Institute, University of Southern Denmark, ³Laboratory of Molecular and Cellular Cardiology, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, ⁴Center for Vascular Regeneration, Odense University Hospital, ⁵Research Unit for Plastic Surgery, Odense University Hospital

Precise disclosure of methods and protocols is crucial for large scale uptake of stem cell therapies. Here, we present a protocol to isolate adipose-derived regenerative cells, used for a single intracavernous injection as treatment of erectile dysfunction (ED) following radical prostatectomy (RP).

Immunology and Infection

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Jeff Granhøj¹, Henrik Dimke^1,2, Per Svenningsen¹

¹Department of Molecular Medicine, University of Southern Denmark, ²Department of Nephrology, Odense University Hospital

Here we present a method to select for novel variants of the E. coli biotin-protein ligase BirA that biotinylates a specific target peptide. The protocol describes the construction of a plasmid for the bacterial display of the target peptide, generation of a BirA library, selection and characterization of BirA variants.

Medicine

A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice

Alexander Wiinholt^1,2, Mads G. Jørgensen², Amar Bučan^1,2, Farima Dalaei^1,2, Jens A. Sørensen²

¹University of Southern Denmark, ²Department of Plastic Surgery, Odense University Hospital

This animal model enables researchers to induce statistically significant secondary lymphedema in the hindlimb of mice, lasting at least 8 weeks. The model can be used to study the pathophysiology of lymphedema and to investigate novel treatment options.

Medicine

Radial Endobronchial Ultrasound and Electromagnetic Navigation Bronchoscopy with Fluoroscopy for the Diagnosis of Peripheral Lung Lesions

Amanda Dandanell Juul^1,2, Alexis Pulga³, Arman Arshad¹

¹Department of Respiratory Medicine, Odense University Hospital, ²Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, ³Department of Respiratory Medicine, Bispebjerg Hospital

Diagnosing small lung tumors is quite difficult using a bronchoscope alone. Electromagnetic navigation bronchoscopy is used to locate the lesion, similar to the Global Positioning System. Radial endobronchial ultrasound and fluoroscopy confirm the correct location and monitor the sampling.

Medicine

Using the Endoscope for Endobronchial Ultrasound in the Esophagus

Mohammad A. Issa¹, Paul F. Clementsen², Christian B. Laursen^3,4, Peter Vilmann^5,6, Ida S. Christiansen⁷, Laurence Crombag⁸, Uffe Bodtger^1,9

¹Pulmonary Research Unit (PLUZ), Department of Respiratory Medicine, Zealand University Hospital, ²Copenhagen Academy for Medical Education and Simulation (CAMES), Copenhagen University Hospital, Rigshospitalet, ³Department of Respiratory Medicine, Odense University Hospital, ⁴Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, ⁵Department of Surgery, Copenhagen University Hospital, ⁶Department of Clinical Medicine, University of Copenhagen, ⁷Department of Pathology, Copenhagen University Hospital, Rigshospitalet, ⁸Department of Respiratory Medicine, University Medical Center, University of Amsterdam, ⁹Institute for Regional Health Research, University of Southern Denmark

Transesophageal ultrasound (EUS-B) is a safe and feasible procedure using the echoendobronchoscope (EBUS) in esophagus and stomach. After identifying six anatomical landmarks, additional structures can be identified and biopsied, sparing subsequent diagnostic sessions. Thus, EUS-B is an ideal continuation of bronchoscopy and EBUS in diagnosing lung cancer and other diseases.

Medicine

Transbronchial Lung Cryobiopsy for Diagnosing Interstitial Lung Diseases and Peripheral Pulmonary Lesions - A Stepwise Approach

Jesper R. Davidsen^1,2, Christian B. Laursen^1,2, Søren H. Skaarup³, Sissel B. Kronborg-White³, Amanda D. Juul^1,2

¹South Danish Center for Interstitial Lung Diseases (SCILS), Department of Respiratory Medicine, Odense University Hospital, ²Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, ³Department of Respiratory Diseases and Allergy, Aarhus University Hospital

Transbronchial lung cryobiopsy (TBLC) for diagnosing interstitial lung disease and peripheral pulmonary lesions is a high-yield diagnostic and safe procedure. We describe a stepwise approach to conduct TBLC for the different indications mentioned with a flexible bronchoscope, which might be helpful for novice bronchoscopists performing TBLC.

Medicine

A Stepwise Approach for Performing Ultrasound Guided Transthoracic Lung Biopsy

Christian B. Laursen^1,2, Rahul Bhatnagar^2,3,4, Amanda Dandanell Juul^1,2

¹Department of Respiratory Medicine, Odense University Hospital, ²Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, ³Respiratory Medicine Department, North Bristol NHS Trust, Southmead Hospital, ⁴Academic Respiratory Unit, University of Bristol

Transthoracic ultrasound-guided lung biopsy represents a safe, cost-effective, and efficient approach for patients presenting with subpleural lung lesions suspected of malignancy. Employing a systematic, step-by-step process is crucial to achieve optimal patient selection, minimize complication risks, and maximize diagnostic accuracy.