Leibniz Institute for Neurobiology
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
Pingan Yuanxiang 1, Sujoy Bera 1, Anna Karpova 1, Michael R. Kreutz 1, Marina Mikhaylova 1,2
1RG Neuroplasticity, Leibniz Institute for Neurobiology, 2Department of Cell Biology, Utrecht University
We provide a detailed protocol for induction of long-term potentiation in the CA1 region of the hippocampus and the subsequent isolation of nuclear enriched fractions from the tetanized area of the slice. This approach can be used to determine activity dependent nuclear protein import in cellular models of learning and memory.
Medicine
Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease
Martin Wagner 1, Claudia Baer 1, Werner Zuschratter 2, Monika Riek-Burchardt 3, Christian Deffge 1, Soenke Weinert 1, Jerry C Lee 4, Ruediger C Braun-Dullaeus 1, Joerg Herold 1
1Department of Cardiology and Angiology, University of Magdeburg, 2Leibniz Institute for Neurobiology, 3Institute of Molecular and Clinical Immunology, University of Magdeburg, 4Division of Hematology/Oncology, Boston Children's Hospital, Harvard Medical School
Monocytes are important mediators of arteriogenesis in the context of peripheral arterial disease. Using a basement membrane-like matrix and intravital microscopy, this protocol investigates monocyte homing and tumor-related angiogenesis after monocyte injection in the femoral artery ligation murine model.
