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Leibniz Institute for Neurobiology

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

Pingan Yuanxiang¹, Sujoy Bera¹, Anna Karpova¹, Michael R. Kreutz¹, Marina Mikhaylova^1,2

¹RG Neuroplasticity, Leibniz Institute for Neurobiology, ²Department of Cell Biology, Utrecht University

We provide a detailed protocol for induction of long-term potentiation in the CA1 region of the hippocampus and the subsequent isolation of nuclear enriched fractions from the tetanized area of the slice. This approach can be used to determine activity dependent nuclear protein import in cellular models of learning and memory.

Medicine

Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease

Martin Wagner¹, Claudia Baer¹, Werner Zuschratter², Monika Riek-Burchardt³, Christian Deffge¹, Soenke Weinert¹, Jerry C Lee⁴, Ruediger C Braun-Dullaeus¹, Joerg Herold¹

¹Department of Cardiology and Angiology, University of Magdeburg, ²Leibniz Institute for Neurobiology, ³Institute of Molecular and Clinical Immunology, University of Magdeburg, ⁴Division of Hematology/Oncology, Boston Children's Hospital, Harvard Medical School

Monocytes are important mediators of arteriogenesis in the context of peripheral arterial disease. Using a basement membrane-like matrix and intravital microscopy, this protocol investigates monocyte homing and tumor-related angiogenesis after monocyte injection in the femoral artery ligation murine model.