Hunan Provincial Maternal and Child Health Care Hospital

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Xiaoman Wang¹, Qian Xie¹, Linling Ke¹, Yuanhang Gong¹, Min Li^1,2

¹Institute of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, ²National Health Commission Key Laboratory of Birth Defect Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital

We present a rapid and efficient method to detect common fragile site breaks through native γH2A.X chromatin immunoprecipitation (ChIP). This approach significantly reduces both the time and labor associated with traditional γH2A.X ChIP assays while maintaining high reproducibility and reliability of results.

Cancer Research

Proximity Ligation Assay to Study Oncogene-Derived Transcription-Replication Conflicts

Linling Ke¹, Qian Xie¹, Xiaoman Wang¹, Yuanhang Gong¹, Min Li^1,2

¹Institute of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, ²National Health Commission Key Laboratory of Birth Defect Research and Preventio, Hunan Provincial Maternal and Child Health Care Hospital

Here, we present a sensitive and specific method to detect oncogene-induced transcription-replication conflicts (TRCs) using the proximity ligation assay (PLA). This approach leverages specific antibodies targeting PCNA and phospho-CTD RNAPII, enabling the assessment of TRC prevalence between RNA polymerase II transcription and DNA replication machinery.