Proximity Ligation Assay to Study Oncogene-Derived Transcription-Replication ConflictsLinling Ke 1, Qian Xie 1, Xiaoman Wang 1, Yuanhang Gong 1, Min Li 1,2
1Institute of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, 2National Health Commission Key Laboratory of Birth Defect Research and Preventio, Hunan Provincial Maternal and Child Health Care Hospital
Here, we present a sensitive and specific method to detect oncogene-induced transcription-replication conflicts (TRCs) using the proximity ligation assay (PLA). This approach leverages specific antibodies targeting PCNA and phospho-CTD RNAPII, enabling the assessment of TRC prevalence between RNA polymerase II transcription and DNA replication machinery.
