harvard college

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Anthos Christofides^1,2, Carol Cao^1,2,3, Rinku Pal^1,2, Halil I. Aksoylar^1,2, Vassiliki A. Boussiotis^1,2

¹Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, ²Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, ³Harvard College

In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.